QNM peak force AFM experiments were carried out on a multimode 8-nanoscope V instrument (Bruker, CA) under pH 6.0 buffer using an MSCT cantilever with its calibrated spring constant designed between 0.01 to 0.03 N/m and peak force setpoints of 100–200 pN; 5 μl drops of fresh cages +/− auxilin, ATP and Hsc70 or Hsc70ΔC were deposited on freshly peeled mica and imaged under the same buffer following routine optimization for biological AFM. Data were analyzed with instrument software (Nanoscope ver8.15, Bruker, CA) and exported as ascii files for further analysis with Excel (Microsoft, Richmond, WA), and displayed with ImageJ (ver 1.4x, NIH, Bethesda, MD). Samples as described for AFM were absorbed for 20 seconds to a Formvar-carbon grid, followed by a rinse and 20 second incubation with 1% uranyl acetate. Grids were wicked, air dried, and examined at 25,000× magnification in a JEOL JEM 1200EX-II.
Nanoscope ver8
Manufactured by Bruker
The Nanoscope ver8.15 is a high-performance atomic force microscope (AFM) system designed for advanced surface characterization. The instrument employs a scanning probe to measure surface topography and other properties at the nanoscale. The core function of the Nanoscope ver8.15 is to provide precise, high-resolution imaging and analysis of a wide range of materials and samples.
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Lab products found in correlation
2 protocols using nanoscope ver8
1
Cages Dynamics via AFM and Cryo-EM
2
Cages Dynamics via AFM and Cryo-EM
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QNM peak force AFM experiments were carried out on a multimode 8-nanoscope V instrument (Bruker, CA) under pH 6.0 buffer using an MSCT cantilever with its calibrated spring constant designed between 0.01 to 0.03 N/m and peak force setpoints of 100–200 pN; 5 μl drops of fresh cages +/− auxilin, ATP and Hsc70 or Hsc70ΔC were deposited on freshly peeled mica and imaged under the same buffer following routine optimization for biological AFM. Data were analyzed with instrument software (Nanoscope ver8.15, Bruker, CA) and exported as ascii files for further analysis with Excel (Microsoft, Richmond, WA), and displayed with ImageJ (ver 1.4x, NIH, Bethesda, MD). Samples as described for AFM were absorbed for 20 seconds to a Formvar-carbon grid, followed by a rinse and 20 second incubation with 1% uranyl acetate. Grids were wicked, air dried, and examined at 25,000× magnification in a JEOL JEM 1200EX-II.
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