Inpro 6800

Manufactured by Mettler Toledo

Sourced in Switzerland

The InPro 6800 is a high-performance dissolved oxygen and temperature sensor for industrial and environmental applications. It is designed to provide accurate and reliable measurements of dissolved oxygen levels in various liquids. The sensor utilizes a galvanic cell technology to measure the dissolved oxygen concentration.

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Lab products found in correlation

Biostat a, by Sartorius (2 mentions) Bioflo 110, by Eppendorf (1 mentions) Sevencompact ph ionmeters s220, by Mettler Toledo (1 mentions) Fmt150, by Photon Systems Instruments (1 mentions) Antifoam, by Merck Group (1 mentions)

8 protocols using inpro 6800

Continuous monitoring of E. coli culture

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Dissolved oxygen and pH in E. coli cultures were continuously measured directly in the flasks using a Clarke oxygen electrode InPro 6800 (Mettler Toledo, Greifensee, Switzerland) and a pH electrode ESC-10601/7 (“IT” Company, Moscow, Russia), respectively. The dO₂/pH controller of a BioFlo 110 fermentor (New Brunswick Scientific Co., Edison, NJ, USA) was used for data recording.
Extracellular sulfide was continuously recorded directly in the flasks using a system of sulfide-specific ion-selective chalcogenide XC-S²-001 (operating pH range 6–12) (Sensor Systems Company, St. Petersburg, Russia) and reference electrodes and a computer pH/ion meter cpX-2 (IBP, Pushchino, Russia).
Changes in levels of extracellular K⁺ were registered using a system of K⁺-selective (ELIS-121K) and reference electrodes. For a sensitive determination of K⁺ during E. coli growth in M9 and M9 + CA + cystine media, potassium concentration was reduced to 0.2 mM. Synchronous processing of all primary data from the sensor system was carried out using the RS-232 and Modbus protocols and the Advantech OPC Server v3.0 software package.

Smirnova G., Tyulenev A., Muzyka N., Ushakov V., Samoilova Z, & Oktyabrsky O. (2023). Influence of Growth Medium Composition on Physiological Responses of Escherichia coli to the Action of Chloramphenicol and Ciprofloxacin. BioTech, 12(2), 43.

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Nitrogen-limited Cultivation of P. putida

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Pseudomonas putida KT2440 (ATCC^® 47054™) was grown in a nitrogen-limited mineral medium consisting of (per liter): 2 g Na₂HPO₄·12H₂O, 2 g KCl, 0.3 g Na₂SO₄, 1 g (NH₄)SO₄, 1 g MgSO₄·7H₂O, and 2.5 mL of trace element solution. Each liter of trace element solution contained: 20 g FeCl₃·6H₂O, 10 g CaCl₂·H₂O, 0.03 g CuSO₄·5H₂O, 0.05 g MnCl₂·4H₂O, and 0.1 g ZnSO₄·7H₂O dissolved in 0.5 N HCl. The cultures were supplemented with sodium gluconate or oleic acid as the only substrate in the same carbon concentration (3.8 g/L) in the production media. The cultivation was carried out in a 5 L working volume in a bioreactor (BioFlo 110, New Brunswick Scientific) at 30 °C with an aeration rate of 4 L/min. pH value was maintained at 7 through the modulated addition of concentrated 1 N NaOH and 1 N HCl. The dissolved oxygen was monitored during the whole cycle with O₂ electrode (InPro 6800, Mettler Toledo GmbH, Switzerland) and maintained 50% air saturation by adjusting the agitation rate from 300 to 1000 rpm automatically. A concentrate solution (Sigma-Aldrich, USA) was used as antifoam in response to the antifoam controller. Total fermentation time was 48 h. In addition, parameters such as biomass, mcl-PHA, nitrogen, and phosphorus concentrations were controlled during the experiments.

Mozejko-Ciesielska J., Pokoj T, & Ciesielski S. (2018). Transcriptome remodeling of Pseudomonas putida KT2440 during mcl-PHAs synthesis: effect of different carbon sources and response to nitrogen stress. Journal of Industrial Microbiology & Biotechnology, 45(6), 433-446.

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Continuous Monitoring of Dissolved Oxygen, pH, and Sulfide in E. coli Cultures

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Dissolved oxygen and pH in E. coli cultures were continuously measured directly in the flasks using a Clarke oxygen electrode InPro 6800 (Mettler Toledo, Greifensee, Switzerland) and a pH electrode ESC-10601/7 (“IT”Company, Moscow, Russia), respectively. The dO₂/pH controller of a BioFlo 110 fermentor (New Brunswick Scientific Co., Edison, NJ, USA) was used for data recording.
Extracellular sulfide was continuously recorded directly in the flasks using the system of sulfide-specific ion-selective chalcogenide XC-S2-001 (operating pH range 6–12) (Sensor Systems Company, St. Petersburg, Russia) and reference electrodes and a computer pH/ion meter cpX-2 (IBI, Pushchino, Russia). The sulfide concentration in the medium was calculated using a standard curve prepared with known amounts of Na₂S. However, it should be taken into account that during the experiments part of the hydrogen sulfide is lost to the gas phase. The synchronous processing of all primary data from the sensor system was carried out using the RS-232 and Modbus protocols and the Advantech OPC Server v3.0 software package.

Smirnova G., Tyulenev A., Sutormina L., Kalashnikova T., Muzyka N., Ushakov V., Samoilova Z, & Oktyabrsky O. (2024). Regulation of Cysteine Homeostasis and Its Effect on Escherichia coli Sensitivity to Ciprofloxacin in LB Medium. International Journal of Molecular Sciences, 25(8), 4424.

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Continuous In-Situ Monitoring of E. coli

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Dissolved oxygen (dO₂) in E. coli BW25113 cultures were continuously measured directly in the flasks using a Clarke oxygen electrode InPro 6800 (Mettler Toledo). The dO₂/pH controller of a BioFlo 110 fermentor (New Brunswick Scientific Co., USA) was used for data recording.
Redox potential (Eh) in the cell-free medium and E. coli cultures was continuously measured directly in the flasks using platinum and reference electrodes and Mettler Toledo SevenCompact™ pH/Ionmeters S220.
Changes in the levels of extracellular K⁺ were continuously registered directly in the flasks using the system of K⁺-selective (ELIS-121K) and reference electrodes and a computer pH/ion meter cpX-2 (IBI Pushchino, Russia). For K⁺ measurements, E. coli cells were grown as described above, except that the medium contained a low K⁺ concentration (0.1 mM).
Extracellular sulfide levels were detected directly in the flasks using the system of sulfide-specific ion-selective XC-S2-001 (Sensor Systems Company, Russia) and reference electrodes and a computer pH/ion meter cpX-2 (IBI Pushchino, Russia).

Samoilova Z., Tyulenev A., Muzyka N., Smirnova G, & Oktyabrsky O. (2019). Tannic and gallic acids alter redox-parameters of the medium and modulate biofilm formation. AIMS Microbiology, 5(4), 379-392.

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Bioreactor Production of Polyhydroxyalkanoates

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Pseudomonas putida KT2440 (ATCC^® 47054™) from long-term storage were firstly grown overnight in lysogeny broth (1% w/v tryptone, 0.5% w/v yeast extract, 1% NaCl) at 30 °C at 220 rpm in a rotary shaker. Then, the bacterial cells were transferred to a mineral medium for PHAs synthesis. The PHAs production medium consisted of (per liter): 3.5 g Na₂HPO₄·12H₂O, 7.0 g KH₂PO₄, 1 g (NH₄)SO₄, 1 g MgSO₄·7H₂O, 10 g sodium gluconate and 2.5 mL of trace element solution. Each liter of trace element solution contained the following components: 20 g FeCl₃·6H₂O, 10 g CaCl₂·H₂O, 0.03 g CuSO₄·5H₂O, 0.05 g MnCl₂·4H₂O, 0.1 g ZnSO₄·7H₂O dissolved in 0.5 N HCl. The cultivation was carried out in a 7 L bioreactor (Biostat A, Sartorius, Germany) at 30 °C with an aeration rate of 4 L/min. pH-value was maintained at 7 through the modulated addition of concentrated 1 N NaOH and 1 N HCl. The dissolved oxygen was monitored during the whole cycle with O₂ electrode (InPro 6800, Mettler Toledo GmbH, Switzerland) and maintained 50% air saturation by adjusting the agitation rate from 300 rpm to 1000 rpm automatically. Total fermentation time was 48 h. Three replicate cultures were conducted.

Możejko-Ciesielska J, & Mostek A. (2019). A 2D-DIGE-based proteomic analysis brings new insights into cellular responses of Pseudomonas putida KT2440 during polyhydroxyalkanoates synthesis. Microbial Cell Factories, 18, 93.

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Photobioreactor Cultivation of N. oculata

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During experiments, N. oculata was cultured in a 400 mL flat panel photobioreactor (FMT-150, Photon Systems Instruments, Brno, Czech Republic). The photobioreactor illumination consisted of red and blue LEDs ((red: λmax ≈ 633 nm, λ1/2 ≈ 20 nm, Luxeon LXHLPD09; white: Luxeon LXHL-PW09; blue: λmax ≈ 445 nm, λ1/2 ≈ 20 nm, Luxeon LXHL-PR09; all manufactured by Future Lighting Solutions, Montreal, QC, Canada). The photobioreactor continuously measured optical density at 680 nm and 720 nm by an inbuilt densitometer and steady-state pigment fluorescence emission yield by an inbuilt fluorometer, both described in detail in [27] (link). The culture was mixed by aeration (200 mL min -1 , instrument grade air, BOC Australia, North Ryde, NSW, AU) complemented by stirring with a magnetic bar. Dissolved O2 was monitored by a microelectrode (InPro6800), pH and temperature were monitored by a combined electrode (InPro3253, both manufactured by MettlerToledo, Inc.).

Zavřel T., Szabó M., Tamburic B., Evenhuis C., Kuzhiumparambil U., Literáková P., Larkum A.W.D., Raven J.A., Červený J, & Ralph P.J. (2018). Effect of carbon limitation on photosynthetic electron transport in Nannochloropsis oculata. Journal of photochemistry and photobiology. B, Biology, 181.

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Optimized Cultivation of Pseudomonas putida KT2440

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The precultures of Pseudomonas putida KT2440 were used to inoculate a 7.0-L bioreactor (Biostat A, Sartorius, Germany) so that the initial OD₆₀₀ was 0.1. The inoculation size was 10%. All cultivations were carried out at 30 °C. The temperature was maintained by a thermostatic jacket. The pH of each culture was maintained at 7.0 through the modulated addition of 1 N NaOH and 1 N HCl. The dissolved oxygen was monitored during the entire cycle with an O₂ electrode (InPro 6800, Mettler Toledo GmbH, Greifensee, Switzerland). The agitation rate from 300 rpm to 1000 rpm was adjusted automatically to maintain 50% air saturation. The total fermentation time was 48 h. An antifoam solution (Sigma Aldrich, St. Louis, MO, USA) was used in response to the antifoam controller.

Możejko-Ciesielska J, & Mostek A. (2019). Time-Course Proteomic Analysis of Pseudomonas putida KT2440 during Mcl-Polyhydroxyalkanoate Synthesis under Nitrogen Deficiency. Polymers, 11(5), 748.

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Acidic-Shock Batch Fermentation of S. albulus

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A 5-L fermenter (BIOTECH-5BG, BaoXing Bio-Engineering Equipment, China) with a 3.5-L working volume and two Rushton turbines (Φ = 6 cm) was employed for batch fermentation in this study. Before the inoculation, temperature, aeration rate and agitation speed were maintained at 30°C, 0.5 vvm and 200 rpm, respectively, and initial pH was controlled at 6.8 via manual addition of ammonia water (12.5%, w/v). Approximately 300 mL of seed culture was used as the inoculum. Dissolved oxygen (DO) was set above 30% of air saturation, which was controlled by manually adjusting agitation speed from 200 to 800 rpm and aeration rate with a range of 0.5-2.5 vvm. During the fermentation process, pH and DO were respectively monitored online by pH and DO electrodes (K8S-225 and InPro6800, Mettler Toledo, Switzerland). To investigate the ATR of S. albulus M-Z18, pH was respectively maintained at 5.0, 4.0 and 3.0 by ammonia water (12.5%, w/v) when it spontaneously dropped from initial 6.8 to the set values, and then the cells were harvested at 27 h (Figure 1A). At this time, it was about 12 hours since pH spontaneously dropped to 4.0, which was in accordance with the acidic-shock time in our previous study (Ren et al., 2015 (link)).

Wang C., Ren X., Yu C., Wang J., Wang L., Zhuge X, & Liu X. (2020). Physiological and Transcriptional Responses of Streptomyces albulus to Acid Stress in the Biosynthesis of ε-Poly-L-lysine. Frontiers in Microbiology, 11, 1379.

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