Anti syk

Syk inhibitors SykI and BAY 61-3606, PI3K inhibitor LY294002, ERK inhibitor U0126, JNK inhibitor SP600125, and p38 inhibitor SB203580 were purchased from Calbiochem-Merck (Darmstadt, Germany). Raf-1 inhibitor GW5074, methyl-β-cyclodextrin (MβCD), and water-soluble cholesterol were obtained from Sigma-Aldrich.
Antibodies against phospho (p)-Zap-70 (Tyr319)/Syk (Tyr352), p-Akt (Tyr308), p-c-Raf (Ser338), p-ERK1/2 (Thr202/Tyr204), p-JNK (Thr183/Tyr185), JNK, p-p38 (Thr180/Tyr182), p-IKKα/β (Ser176/180), p-NF-κBp65 (Ser536), p-IκBα (Ser32), IκBα, p-c-Jun (Ser63), c-Jun, p-c-Fos (Ser32), and c-Fos were purchased from Cell Signaling (Beverly, MA, USA). Anti-Syk, anti-β-actin, HRP-conjugated anti-rabbit IgG, and HRP-conjugated anti-mouse IgG antibodies were purchased from GeneTex Inc. (Irvine, CA, USA). Blocking antibodies against CR3 (clone 5C6) and Dectin-1 (clone 2A11) were purchased from Serotec (Oxford, UK). Antibodies for cell surface staining, anti-CD11b (clone M1/70), anti-CD18 (clone GAME-46), and APC-conjugated anti-F4/80 antibody were obtained from eBioscience (San Diego, CA, USA), and anti-Dectin-1 (clone 218820) was purchased from R&D Systems (Minneapolis, MN, USA).

Syk inhibitors SykI and BAY 61-3606, JNK inhibitor SP600125, ERK inhibitor U0126, and p38 inhibitor SB203580 were obtained from Calbiochem-Merck. Laminarin and zymosan were purchased from InvivoGen. Peptide inhibitor for NADPH oxidase assembly, gp91ds-tat, was purchased from AnaSpec. Diphenyleneiodonium chloride (DPI) and Mito-TEMPO were obtained from Sigma-Aldrich. LysoTracker Red was obtained from Life Technologies.
Antibodies against LC3, Rubicon, NLRX1, phospho (p)-p40-phox (Thr154), p-JNK (Thr183/Tyr185), p-ERK1/2 (Thr202/Tyr204), p-p38 (Thr180/Tyr182), p-c-Fos (Ser32), p-IKKα/β (Ser176/180), p-IκBα (Ser32), IκBα, and p-NFκBp65 (Ser536) were purchased from Cell Signaling. Antibodies against p-Syk (Tyr525), p-c-Jun (Ser63), and TUFM were obtained from Abcam. Anti-Syk, anti-ATG5, anti-β-actin, HRP-conjugated anti-rabbit IgG, and Rabbit IgG isotype control were purchased from GeneTex Inc. Receptor blocking antibodies against Dectin-1 (clone 2A11), Dectin-2 (clone D2.11E4), and CR3 (clone 5C6) were purchased from Bio-Rad (formerly AbD Serotec) and TLR2 (clone 6C2) was from eBioscience.