Mouse monoclonal anti nucleolin c23

Manufactured by Santa Cruz Biotechnology

The Mouse monoclonal anti-nucleolin(C23) is a laboratory reagent that can be used to detect and study the nucleolin protein in mouse samples. Nucleolin is a multifunctional protein involved in various cellular processes. This antibody can be used in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to identify and analyze the nucleolin protein.

Automatically generated - may contain errors

Lab products found in correlation

Ab15289, by Abcam (1 mentions) Anti cyclin d1, by Cell Signaling Technology (1 mentions) Human fgf19 quantikine elisa kit, by R&D Systems (1 mentions) Rabbit polyclonal anti parp, by Cell Signaling Technology (1 mentions) Mouse monoclonal anti brdu, by BD (1 mentions) Mouse monoclonal anti ki 67, by BD (1 mentions)

2 protocols using mouse monoclonal anti nucleolin c23

Detailed Immunoblotting Procedure

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunoblotting was performed as described.^{12 (link),13 (link)} The following antibodies were used: rabbit polyclonal anti-CIMPR
(ab32815, Abcam); mouse monoclonal anti-cyclin D1 (2926, Cell Signaling
Technologies); mouse monoclonal anti-FLAG (M2, Sigma-Aldrich); rabbit
polyclonal anti-FLAG (Immunology Consultants Laboratory, Inc.); rabbit
polyclonal anti-Fli-1 (ab15289, Abcam); mouse monoclonal anti-HA (16B12,
Covance); mouse monoclonal anti-LAMP2 (55803, BD Biosciences); rabbit
polyclonal anti-mSin3A (K-20, Santa Cruz Biotechnology); rabbit polyclonal
anti-Myc (N262, Santa Cruz Biotechnology); mouse monoclonal anti-nucleolin
(C23, Santa Cruz Biotechnology); mouse monoclonal anti-p62/SQSTM1
(610832, BD Biosciences); and mouse monoclonal anti-tubulin (DM1A,
Lab Vision).

Elzi D.J., Song M., Hakala K., Weintraub S.T, & Shiio Y. (2014). Proteomic Analysis of the EWS-Fli-1 Interactome Reveals the Role of the Lysosome in EWS-Fli-1 Turnover. Journal of Proteome Research, 13(8), 3783-3791.

+ Open protocol

+ Expand

Immunoblotting and Immunofluorescence Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunoblotting and immunofluorescence were performed as described [22 (link), 23 (link)]. FGF19 protein levels in the medium were determined using human FGF-19 Quantikine ELISA Kit (R&D Systems). The following antibodies were used: mouse monoclonal anti-BrdU (BD Pharmingen); mouse monoclonal anti-Ki-67 (BD Pharmingen); mouse monoclonal anti-nucleolin (C23, Santa Cruz Biotechnology); rabbit polyclonal anti-PARP (#9542, Cell Signaling); mouse monoclonal anti-phospho-ERK (E4, Santa Cruz Biotechnology); rabbit polyclonal anti-phospho-FRS2 (Tyr196) (#3864, Cell Signaling); mouse monoclonal anti-FRS2 (MAB4069, R&D Systems); and mouse monoclonal anti-RB (G3-245, BD Pharmingen).

Elzi D.J., Song M., Blackman B., Weintraub S.T., López-Terrada D., Chen Y., Tomlinson G.E, & Shiio Y. (2016). FGF19 functions as autocrine growth factor for hepatoblastoma. Genes & Cancer, 7(3-4), 125-135.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!