Hcc827

Manufactured by BNCC

Sourced in China

The HCC827 is a high-performance laboratory equipment designed for a variety of scientific applications. It features precise temperature control, advanced data monitoring, and reliable performance. The core function of the HCC827 is to provide a controlled environment for various experiments and research activities.

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Lab products found in correlation

7 protocols using hcc827

Culturing NSCLC and Bronchial Cell Lines

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The HCC827, H522, and H23 NSCLC cell lines, and the 16HBE bronchial epithelial cell were purchased from BeNa Culture Collection. HCC827, H522 and H23 were cultured in RPMI-1640 supplemented with 10% FBS (both from Gibco; Thermo Fisher Scientific, Inc.) at 37°C containing 5% CO₂. 16HBE cells were cultured in DMEM (Gibco; Thermo Fisher Scientific, Inc.) and 10% FBS at 37°C containing 5% CO₂.

Luo S., Shen M., Chen H., Li W, & Chen C. (2020). Long non-coding RNA TP73-AS1 accelerates the progression and cisplatin resistance of non-small cell lung cancer by upregulating the expression of TRIM29 via competitively targeting microRNA-34a-5p. Molecular Medicine Reports, 22(5), 3822-3832.

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Cell Culture of Human Cell Lines

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Human embryo kidney cell line HEK-293T, human NSCLC cell line SK-MES-1 (with TP53 and EGFR mutation), A549, and HCC827, human normal lung epithelial cell line BEAS-2B, and human umbilical vein endothelial cells (HUVECs) were purchased from BeNa Culture Collection (Beijing, China). HEK-293T and SK-MES-1 cells were cultured with Dulbecco modified Eagle medium (DMEM; Gibco, Grand Island, New York) containing 10% fetal bovine serum (FBS; Gibco), BEAS-2B and HCC827 cells were cultured with RPMI-1640 medium (Gibco) containing 10% FBS, A549 cells and HUVEC were cultured with Ham F12K medium (Gibco) containing 10% FBS. All the cells were incubated at 37°C in a humidified chamber containing 5% CO₂.

Jiang S., Liu X., Li D., Yan M., Ju C., Sun J, & Jiang F. (2018). Study on Attenuating Angiogenesis and Epithelial–Mesenchymal Transition (EMT) of Non-Small Cell Lung Carcinoma (NSCLC) by Regulating MAGEC2. Technology in Cancer Research & Treatment, 17, 1533033818797587.

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Cell Culture Conditions for Lung Cells

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The normal human lung epithelial cells, BEAS-2B, and cancer cell lines, H125, HCC827 and A549, were obtained from the BeNa Culture Collection (Beijing, China). BEAS-2B and A549 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM)/F12 containing 10% heat-inactivated fetal bovine serum, 100 IU/ml penicillin, and 10 g/ml streptomycin. The H125 and HCC827 cell lines were both incubated in RPMI1640 medium containing 10% heat-inactivated fetal bovine serum, 100 IU/ml penicillin, and 10 g/ml streptomycin. All cell lines were incubated in a 95% air and 5% carbon dioxide (CO₂) atmosphere at 37 °C.

Qian Z., Zhang Q., Hu Y., Zhang T., Li J., Liu Z., Zheng H., Gao Y., Jia W., Hu A., Li B, & Hao J. (2018). Investigating the mechanism by which SMAD3 induces PAX6 transcription to promote the development of non-small cell lung cancer. Respiratory Research, 19, 262.

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Culturing Lung Epithelial and NSCLC Cells

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Normal lung epithelial cells (BEAS-2B) and NSCLC cells (A549, H1975, H522, and HCC827) were obtained from BeNa Culture Collection (Beijing, China). All cells were maintained in a humidified chamber at 37°C with 5% CO₂. These NSCLC cell lines were cultured in RPMI (Roswell Park Memorial Institute)-1640 medium (Thermo Fisher, Waltham, MA, USA) supplemented with 10% FBS (fetal bovine serum) (Thermo Fisher) and 1% P/S (penicillin/streptomycin) (Sigma, St. Louis, MO, USA), but BEAS-2B cells were cultured in basal BEGM (bronchial epithelial cell growth medium) (Lonza, Walkersville, MD, USA) supplemented with standardized growth factors (BEGM BulletKit, Lonza).

Dou Y., Tian W., Wang H, & Lv S. (2021). Circ_0001944 Contributes to Glycolysis and Tumor Growth by Upregulating NFAT5 Through Acting as a Decoy for miR-142-5p in Non-Small Cell Lung Cancer. Cancer Management and Research, 13, 3775-3787.

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Lung Adenocarcinoma Cell Culture Protocol

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Lung adenocarcinoma cell line PC9 and HCC827 were from BeNa Culture Collection (BNCC, China). Cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin at 37°C in a humidified atmosphere of 5% CO₂ [17 (link)]. For the main tests, cells were treated with erlotinib (final concentration 2 μM) for 24 h or pretreated with lactate (10 mM) for 12 h before erlotinib.

Ma R., Li X., Gong S., Ge X., Zhu T., Ge X., Weng L., Tao Q, & Guo J. (2022). Dual Roles of Lactate in EGFR-TKI-Resistant Lung Cancer by Targeting GPR81 and MCT1. Journal of Oncology, 2022, 3425841.

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Culturing Human Bronchial and Lung Cancer Cells

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The normal human bronchial epithelium cell line BEAS-2B and NSCLC cell lines A549 and HCC827 were purchased from BeNa Culture Collection (BNCC; Suzhou, P.R. China). All cell lines were cultured in high-glucose Dulbecco’s modified Eagle’s medium (H-DMEM) with 10% fetal bovine serum (FBS; Sigma-Aldrich, Cambridge, MA, USA).

Yang H., Yan L., Sun K., Sun X., Zhang X., Cai K, & Song T. (2019). lncRNA BCAR4 Increases Viability, Invasion, and Migration of Non-Small Cell Lung Cancer Cells by Targeting Glioma-Associated Oncogene 2 (GLI2). Oncology Research, 27(3), 359-369.

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Cell Line Maintenance and Culture

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Human bronchial epithelial cell line (HBE1) and a panel of four NSCLC cell lines (A549, 95-D, HCC827, and H1299) were obtained from BeNa Culture Collection (Beijing, China). All cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Carlsbad, CA, USA) added with 10% fetal bovine serum (FBS; Gibco) and penicillin/streptomycin (100 units/mL) at 37℃ in a 5% CO₂ environment.

Ma X., Wang C., Chen J., Wei D., Yu F, & Sun J. (2021). circAGFG1 sponges miR-28-5p to promote non-small-cell lung cancer progression through modulating HIF-1α level. Open Medicine, 16(1), 703-717.

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