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Ast and alt activity assay kit

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The AST and ALT Activity Assay Kit is a laboratory tool used to measure the levels of the enzymes aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in biological samples. The kit provides a quantitative and colorimetric method for detecting these enzymes, which are commonly used as biomarkers for various health conditions.

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Lab products found in correlation

Au2700 analyzer, by Diamond Diagnostics (1 mentions)

Close spelling variants of this product

ALT and AST kits AST activity assay ALT Activity Assay ALT activity kit AST activity Activity Assay Kits AST kit ALT kit

7 protocols using ast and alt activity assay kit

1

Liver and Kidney Function Assays

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Blood samples were collected when animals were killed. After centrifugation, asparate aminotransferase (AST) and alanine aminotransferase (ALT) concentrations were measured using AST and ALT Activity Assay Kit (Sigma Aldrich). Serum urea and creatinine concentrations were measured by using an Olympus AU2700 analyzer (Diamond Diagnostics, Watford, UK).
Zhao H., Huang H., Alam A., Chen Q., Suen K.C., Cui J., Sun Q., Ologunde R., Zhang W., Lian Q, & Ma D. (2018). VEGF mitigates histone‐induced pyroptosis in the remote liver injury associated with renal allograft ischemia–reperfusion injury in rats. American Journal of Transplantation, 18(8), 1890-1903.
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2

Serum ALT and AST Measurement

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Blood obtained by cardiac puncture was collected for measurements of serum alanine aminotransferase (ALT), and aspartate aminotransferase (AST) concentrations using an AST and ALT Activity Assay Kit (Sigma-Aldrich, St. Louis, MO).
Rahman K., Liu Y., Kumar P., Smith T., Thorn N.E., Farris A.B, & Anania F.A. (2016). C/EBP homologous protein modulates liraglutide-mediated attenuation of non-alcoholic steatohepatitis. Laboratory investigation; a journal of technical methods and pathology, 96(8), 895-908.
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3

Serum ALT and AST Quantification

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Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) concentrations were measured using an AST and ALT Activity Assay Kit (Sigma-Aldrich, St. Louis, MO, USA).
Gupta B., Rai R.P., Pal P.B., Rossmiller D., Chaudhary S., Chiaro A., Seaman S., Singhi A.D., Liu S., Monga S.P., Iyer S.S, & Raeman R. (2024). Selective Targeting of α4β7/MAdCAM-1 Axis Suppresses Fibrosis Progression by Reducing Proinflammatory T Cell Recruitment to the Liver. Cells, 13(9), 756.
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4

Serum ALT and AST Measurement

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Blood obtained by cardiac puncture was collected for measurements of serum alanine aminotransferase (ALT), and aspartate aminotransferase (AST) concentrations using an AST and ALT Activity Assay Kit (Sigma-Aldrich, St. Louis, MO).
Rahman K., Liu Y., Kumar P., Smith T., Thorn N.E., Farris A.B, & Anania F.A. (2016). C/EBP homologous protein modulates liraglutide-mediated attenuation of non-alcoholic steatohepatitis. Laboratory investigation; a journal of technical methods and pathology, 96(8), 895-908.
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5

Serum Enzyme Activity Assay

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Serum AST and ALT activity were determined by commercial AST and ALT activity assay kits (Sigma), respectively.
Yang W., Kim D.M., Jiang W., Ai W., Pan Q., Rahman S., Cai J.J., Brashear W.A., Sun Y, & Guo S. (2023). Suppression of FOXO1 attenuates inflamm‐aging and improves liver function during aging. Aging Cell, 22(10), e13968.
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6

Serum Enzyme Assay in Mice

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Prior to sacrificing of mice, 0.5 mL of blood was collected and serum was separated by centrifugation at 3000 rpm for 20 min. Serum AST and ALT levels were measured using AST and ALT Activity Assay Kits (Sigma-Aldrich).
Amini-Nik S., Sadri A.R., Diao L., Belo C, & Jeschke M.G. (2018). Accumulation of myeloid lineage cells is mapping out liver fibrosis post injury: a targetable lesion using Ketanserin. Experimental & Molecular Medicine, 50(7), 1-13.
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7

Serum ALT and AST Quantification

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AST and ALT Activity Assay Kits (Sigma-Aldrich, St. Louis, MO) were used to estimate serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) concentrations.
Gupta B., Liu Y., Chopyk D.M., Rai R.P., Desai C., Kumar P., Farris A.B., Nusrat A., Parkos C.A., Anania F.A, & Raeman R. (2020). Western diet-induced increase in colonic bile acids compromises epithelial barrier in nonalcoholic steatohepatitis. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(5), 7089-7102.
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