For separation of proteins in native agarose gels, recombinant VEGF (Life Technologies, Carlsbad, California), histones from calf thymus (Sigma-Aldrich, Steinheim, Germany) or catalase from bovine liver (Serva, Heidelberg, Germany) were each incubated with polySia (colominic acid from E. coli equal to polySia in mammals; GERBU, Heidelberg, Germany) in 50 mM Tris buffer for 1 h at 30 °C with agitation. The samples were loaded onto a 2% agarose gel (peqGOLD Universal Agarose, peqLab, Erlangen, Germany) and separated using 19.2 mM glycine in 25 mM Tris/HCl (pH 8.5) buffer. The electrophoresis was run at 80 V (constant voltage) for 3.5 h [32 (link)]. Thereafter, the gel was fixed in 45% methanol containing 7.5% acetic acid (v/v) overnight. Roti-blue (Roth, Karlsruhe, Germany) was used as Coomassie blue staining-dye according to manufacturer’s instructions.
Catalase from bovine liver
Manufactured by Serva Electrophoresis
Sourced in Germany
Catalase from bovine liver is an enzyme that catalyzes the decomposition of hydrogen peroxide to water and oxygen. It is a common laboratory reagent used in various applications, such as in the study of oxidative stress and antioxidant defense mechanisms.
Automatically generated - may contain errors
Lab products found in correlation
Vascular endothelial growth factor (vegf), by Thermo Fisher Scientific (1 mentions)
Histones from calf thymus, by Merck Group (1 mentions)
Peqgold universal agarose, by Avantor (1 mentions)
Roti blue, by Carl Roth (1 mentions)
Anhydrous sodium carbonate, by Merck Group (1 mentions)
Manganese 2 chloride tetrahydrate, by Merck Group (1 mentions)
Ethylenediaminetetraacetic acid disodium salt dihydrate na2edta, by Merck Group (1 mentions)
Gibco minimum essential medium, by Thermo Fisher Scientific (1 mentions)
Gibco fetal bovine serum, by Thermo Fisher Scientific (1 mentions)
Gibco 0.05 trypsin edta, by Thermo Fisher Scientific (1 mentions)
Gibco pen strep, by Thermo Fisher Scientific (1 mentions)
Ringer s solution, by Fresenius (1 mentions)
Bovine serum albumin fraction 5, by Merck Group (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
2 protocols using catalase from bovine liver
1
Protein Separation in Native Agarose Gels
2
Cell Culture Reagents and Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Bovine serum albumin fraction V (BSA), Manganese(II) chloride tetrahydrate, Ethylenediaminetetraacetic acid disodium salt dihydrate (Na2EDTA) and Triton X-100 were purchased from Sigma-Aldrich (Darmstadt, Germany); Sodium carbonate anhydrous and Sodium hydroxide were purchased from Merck (Darmstadt, Germany); Disodium phosphate dehydrate was purchased from Carl Roth (Karlsruhe, Germany); Ringer's solution was purchased from Fresenius Kabi (Bad Homburg, Germany); Gibco Minimum Essential Medium (MEM), Gibco Fetal bovine serum (FBS), Gibco Pen Strep and Gibco 0.05% Trypsin-EDTA were purchased from Thermo Fisher Scientific (Schwerte, Germany); Catalase from bovine liver was purchased from SERVA (Heidelberg, Germany); Genipin was purchased from TCI Chemicals (Eschborn, Germany). All materials were of highest quality available.
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