Ck mb

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CK-MB is a laboratory test used to measure the levels of creatine kinase-MB, an enzyme found primarily in the heart muscle. It is commonly used as a biomarker to help diagnose and monitor certain heart conditions, such as myocardial infarction (heart attack).

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Lab products found in correlation

P gsk 3β, by MyBioSource (2 mentions) Pentobarbital sodium, by Merck Group (1 mentions) Tnf α, by Santa Cruz Biotechnology (1 mentions) Alanine transaminase activity assay kit, by Merck Group (1 mentions) Mda assay kit, by Merck Group (1 mentions) Kits for the quantification of, by MyBioSource (1 mentions) Caspase 3, by MyBioSource (1 mentions) Interleukin 6 (il 6), by MyBioSource (1 mentions) Il 10, by MyBioSource (1 mentions) Ldh cytotoxicity assay kit, by Cayman Chemical (1 mentions) P akt, by MyBioSource (1 mentions)

7 protocols using ck mb

Cardioprotective Effects of Antioxidant Pathways

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The experimental protocol was approved by Institutional Ethical Committee of
Jiangxi Provincial People’s Hospital, with ethic number: 202005088779C09. Wistar
albino rats (200–250g) were used for this study and the animals were kept in the
standardized laboratory facilities. The kits for estimating the levels of
creatine kinase (CK-MB), cardiac troponin T (cTnT), HIF-1α and Nrf-2 were
procured from MyBioSource, Inc., San Diego, CA, USA.

Wang L., Tang Y., He H, & Wei W. (2021). Liraglutide restores late cardioprotective effects of remote preconditioning in diabetic rats via activation of hydrogen sulfide and nuclear factor erythroid 2-related factor 2 signaling pathway. Acta Cirúrgica Brasileira, 36(2), e360207.

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Cardiac Biomarker Analysis in Mice

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Following the measurement of echocardiographic parameters, all mice were anesthetized via intraperitoneal injection of pentobarbital sodium (35 mg/kg body weight; Sigma-Aldrich; Merck KGaA). Blood samples (1 ml) were collected from the abdominal vena cava, transferred to 10-ml centrifuge tubes and maintained at 4°C overnight. Subsequently, the mice were sacrificed via exsanguination under anesthesia. The serum samples were prepared by 30-min centrifugation of blood samples at 2,000 × g at 4°C. The serum levels of cardiac injury biomarkers were measured using commercial ELISA kits, including lactate dehydrogenase (LDH; cat. no. MBS2018912; MyBioSource, Inc.), α-hydroxybutyrate dehydrogenase (α-HBDH; cat. no. MBS9303787; MyBioSource, Inc.), creatine kinase isoenzyme MB (CK-MB; cat. no. A77886; Antibodies.com), cardiac troponin I (cTnI; cat. no. B52699; Beckman Coulter, Inc.) and cardiac troponin T (cTnT; cat. no. MBS 726068; MyBioSource, Inc.).

Li L., Fang H., Yu Y.H., Liu S.X, & Yang Z.Q. (2021). Liquiritigenin attenuates isoprenaline-induced myocardial fibrosis in mice through the TGF-β1/Smad2 and AKT/ERK signaling pathways. Molecular Medicine Reports, 24(4), 686.

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Curcumin-based Enzyme Activity Assays

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DOX (Sigma-Aldrich in St. Louis, MO (USA).
Powdered Curcuma longa (turmeric) was used to make CMX (CAS Number: 458-37-7; Sigma). Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) (ab105134).
Aspartate Aminotransferase Activity Assay Kit (ab105135).
MDA (Assay Kit) (Sigma-Aldrich in St. Louis, MO (USA)).
Abcam provided the CAT (ab83464 Assay Kit) and SOD (ab65354 Assay Kit). TNF-α: derived from Santa Cruz Biotechnology Inc., Dallas, Texas (sc-28318).
Serum CK-MB (Catalogue No.: MBS2515061) and troponin (Catalogue No.: MBS038739) were determined using specific ELISA kits supplied by MyBioSource (San Diego, USA).

Shati A.A., Eid R.A., El-kott A.F., Alqahtani Y.A., Shatoor A.S, & Ahmed Zaki M.S. (2024). Curcumin attenuates doxorubicin-induced cardiotoxicity via suppressing oxidative Stress, preventing inflammation and apoptosis: Ultrastructural and computational approaches. Heliyon, 10(5), e27164.

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Cardioprotective Effects of CCK-8 in Rats

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The experiments were approved by the Central Hospital Affiliated to Shandong First Medical University, under the number: 2020-0618-01. All experiments were performed as per institutional ethical guidelines in the Research Center of the Central Hospital Affiliated to Shandong First Medical University, Jinan, China.
In this investigation, Wistar albino rats of weight 210-240g were used. The doses of CCK-8¹⁴, Proglumide¹⁵, heaxmethonium¹⁶ were selected as per literature reports. The kits for the quantification of lactate dehydrogenase 1 (LDH-1), MB isoform of creatine kinase (CK-MB), cardiac troponins (cTnT), Bcl-2, caspase 3 and CCK were procured from MyBioSource, Inc. San Diego, CA USA.

Li H, & An C. (2020). Exploring the role of neurogenic pathway-linked cholecystokinin release in remote preconditioning-induced cardioprotection. Acta Cirúrgica Brasileira, 35(9), e202000906.

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Cardio-protective Mechanisms in Rats

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Male Wistar albino rats (200–250 g) were used for this investigation. The
experiments were approved by the Ethic Committee of Qingdao Municipal Hospital
with ethical approval number 2020042. The experiments were conducted as per
ethical guidelines in Department of Cardiology, Qingdao Municipal Hospital,
Qingdao, China. The kits for the estimation of cTnT, CK-MB, IL-6, IL-10,
p-GSK-3β and p-NF-kB were purchased from MyBioSource (San Diego, CA, USA).

Xing C., Xu L, & Yao Y. (2021). Beneficial role of oleuropein in sepsis-induced myocardial injury. Possible Involvement of GSK-3β/NF-kB pathway. Acta Cirúrgica Brasileira, 36(1), e360107.

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Cardiac Biomarker Quantification in Serum

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An enzyme-linked immunosorbent assay (ELISA) was performed to measure the levels of cardiac troponin I (cTnI) (Abcam, Cambridge, UK) and creatine kinase isoenzyme MB (CK-MB) (MyBioSource, Inc., San Diego, CA, USA) in serum. The lactate dehydrogenase (LDH) activity in serum was determined by chemical colorimetry using an LDH Cytotoxicity Assay Kit (Cayman Chemical Company, Ann Arbor, Michigan, USA).

Han X., Chen D., Liufu N., Ji F., Zeng Q., Yao W, & Cao M. (2020). MG53 Protects against Sepsis-Induced Myocardial Dysfunction by Upregulating Peroxisome Proliferator-Activated Receptor-α. Oxidative Medicine and Cellular Longevity, 2020, 7413693.

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Cardioprotective Effects of Pharmacological Modulators

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Three-month-old male Wistar albino rats weighing 210-240 g were used in this study. The animals were fed a normal pellet diet containing fiber (5%), protein (20%) and fat (5-10%). Ultraviolet radiation-treated water was provided in bottles for drinking purposes. The animals were exposed to 12 h of light and 12 h of dark at 25 ±2°C and 55-60% relative humidity. The doses of CCK-8, 17 PI3K inhibitor LY294002 22, (link)23 (link) and HIF-1 inhibitor YC-1 24 (link) were determined based on the previous literature. Kits for the quantification of lactate dehydrogenase 1 (LDH-1), the MB isoform of creatine kinase (CK-MB), cardiac troponins (cTnT), HIF-1α, p-Akt, and p-GSK-3β were procured from MyBioSource, Inc. (San Diego, USA).

Cheng Y., Wu M., Liu M., Zhou B., Lin X, & Wang B. (2021). Cholecystokinin-mediated pharmacological preconditioning effects on ischemic rat hearts: Possible signaling pathways. Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 30(11).

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