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Anti adam10

Manufactured by Santa Cruz Biotechnology
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Sourced in Japan, United States

Anti-ADAM10 is a laboratory reagent used for the detection and quantification of ADAM10, a disintegrin and metalloproteinase domain-containing protein, in various biological samples. It is commonly used in research applications for studying cellular processes and molecular pathways involving ADAM10.

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Lab products found in correlation

Anti cd44, by Merck Group (1 mentions) Anti β actin, by Santa Cruz Biotechnology (1 mentions) Anti α tubulin, by Cell Signaling Technology (1 mentions) Cy5 conjugated anti mouse igg, by Jackson ImmunoResearch (1 mentions) Anti cd81, by BioLegend (1 mentions) Anti cd63, by BioLegend (1 mentions) Tritc conjugated anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Anti acetylated α tubulin, by Santa Cruz Biotechnology (1 mentions) Ripa buffer, by Beyotime (1 mentions) Anti bcl 2, by Santa Cruz Biotechnology (1 mentions) Anti ki67, by Santa Cruz Biotechnology (1 mentions) Anti aggrecan, by Santa Cruz Biotechnology (1 mentions) Horseradish peroxidase conjugated anti igg, by Santa Cruz Biotechnology (1 mentions) Anti β actin, by Merck Group (1 mentions) Revert 700 total protein stain solution, by LI COR (1 mentions)

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ADAM10 (6 citations)

4 protocols using anti adam10

1

Immunostaining of Extracellular Vesicle Markers

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The following primary Abs were employed throughout this study: anti-CD9 clone P1/33/2 (Santa Cruz, Dallas, TX), anti-CD9 clone MEM-61 (Abcam, San Francisco, CA), anti-CD9 clone M-L13 (BD Biosciences, San Jose, CA), anti-CD9 clone HI9a (BioLegend, San Diego, CA), anti-CD81 (cat. #349502, BioLegend), anti-CD63 (cat. #sc-15363, Santa Cruz Biotechnology, Dallas, TX), anti-ADAM-10 (cat. #sc-25578, Santa Cruz Biotechnology), anti-β-actin (cat. #sc-47778, Santa Cruz Biotechnology), anti-IgSF8 (cat. #103561, Santa Cruz Biotechnology), anti-β1 integrin (cat #4706S, Cell Signaling Technology, Danvers, MA), anti-α-tubulin (cat. #sc-8035, Santa Cruz Biotechnology), anti-acetylated-α-tubulin (cat. #T7451, Sigma), anti-CD44 (cat. #MA5-13890, ThermoScientific, Waltham, MA). The following secondary Abs were employed: TRITC-conjugated anti-rabbit IgG (Jackson ImmunoResearch), TRITC-conjugated anti-goat IgG (Sigma), and Cy5-conjugated anti-mouse IgG (Jackson ImmunoResearch). For inhibition experiments, sodium azide was removed by desalting through Sephadex G-25 spin columns.
Rappa G., Green T.M., Karbanová J., Corbeil D, & Lorico A. (2015). Tetraspanin CD9 determines invasiveness and tumorigenicity of human breast cancer cells. Oncotarget, 6(10), 7970-7991.
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2

Quantifying ADAM10 and sAPPα with Antibodies

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The following antibodies were used: anti-sAPPα 2B3 (IBL, Gunma, Japan; RRID:AB_1630819) and anti-ADAM10 (Santa Cruz Biotechnology, Santa Cruz, CA; RRID:AB_626635). The following recombinant proteins were used: human mature BDNF (Preprotech, Rocky Hill, NJ) and human sAPPα (Sigma Aldrich, St. Louis, MO). Note that we used mature BDNF and not pro-BDNF, and therefore the Val66Met polymorphism has no bearing on our findings.
Nigam S.M., Xu S., Kritikou J.S., Marosi K., Brodin L, & Mattson M.P. (2017). Exercise and BDNF reduce Aβ production by enhancing α-secretase processing of APP. Journal of neurochemistry, 142(2), 286-296.
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3

Western Blot Analysis of Cartilage Markers

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The cartilage samples and C-28/I2 cells were solubilized in RIPA buffer (Beyotime, Shanghai, China) and Western blot analyses were implemented after SDS polyacrylamide gel electrophoresis as previously reported (Xie et al. 2020) (link). The primary antibodies used for immunoblotting were anti-type II collagen (anti-Col2A1, sc-52658; dilution 1:500), anti-Aggrecan (sc-33695; dilution 1:1000), anti-B cell lymphoma 2 (anti-Bcl-2, sc-7382; dilution 1:500), anti-ki-67 (sc-23900; dilution 1:1000), anti-ADAM10 (sc-48400; dilution 1:500) and a loading buffer anti-glyceraldehyde-3-phosphate dehydrogenase (anti-GAPDH, sc-47724; dilution 1:1000); all from Santa Cruz Biotechnology, Santa Cruz, CA, USA. Horseradish peroxidase conjugated anti-IgG (sc-2748; dilution 1:1000; Santa Cruz Biotechnology) was used as secondary antibody. The signals were developed using the Chemiluminescent Kit (Beyotime) as recommended by manufacturers.
Zhao J., Li T, & Luo W. (2021). Silencing of circ-PRKCH protects against lipopolysaccharide (LPS)-evoked chondrocyte damage and extracellular matrix loss by the miR-140-3p/ADAM10 axis. General physiology and biophysics, 40(2).
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4

Western Blot Analysis of sFRP1 and ADAM10

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Western blot analyses were conducted under reducing conditions as described before [23 (link)]. Specially, 16 μg protein of each TBS-T fraction and 1.2 μg protein of each FA fraction were loaded per well. The primary antibodies and their dilutions used in this study are as follows: anti-sFRP1 (Invitrogen, MA5–38193, 1:1000), anti-ADAM10 (Santa Cruz Biotechnology, SC-28358, 1:1000) and anti-β-actin (Sigma Life Sciences, A2228, 1:5000). The membrane for detecting sFRP1 in FA fraction was restained with Revert 700 total protein stain solution (LI-COR Biosciences) for total protein quantification.
Macyczko J.R., Wang N., Lu W., Jeevaratnam S., Shue F., Martens Y., Liu C.C., Kanekiyo T., Bu G, & Li Y. (2023). Upregulation of sFRP1 is more profound in female than male 5xFAD mice and positively associated with amyloid pathology. Journal of Alzheimer's disease : JAD, 95(2), 399-405.
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