Astra v 6

Manufactured by Wyatt Technology

Sourced in United States, France

The ASTRA v. 6 software is a comprehensive data analysis tool for light scattering measurements. It provides advanced algorithms and data processing capabilities to analyze the results of experiments conducted with Wyatt Technology's light scattering instruments.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

ASTRA 6 software (413 citations) Astra software v. 6.1 (5 citations)

23 protocols using astra v 6

SEC-MALS Analysis of DBB and DBB-ANK

Check if the same lab product or an alternative is used in the 5 most similar protocols

For size exclusion chromatography combined with multiangle light scattering (SEC-MALS) analysis of DBB and DBB-ANK domains, 50-μl protein samples at a concentration of 2 mg/ml were injected onto a Superdex 200 Increase 10/300 GL column (GE Healthcare). Differential refractometry and multiangle light scattering data were collected using Optilab T-rEX (Wyatt technology) and DAWN8⁺ (Wyatt technology) instruments, respectively. Data analysis was performed using ASTRA (v6.1) software (Wyatt technology).

Lauenstein J.U., Scherm M.J., Udgata A., Moncrieffe M.C., Fisher D.I, & Gay N.J. (2020). Negative Regulation of TLR Signaling by BCAP Requires Dimerization of Its DBB Domain. The Journal of Immunology Author Choice, 204(8), 2269-2276.

+ Open protocol

+ Expand

Oligomeric State Analysis of TtEst2 by MALS

Check if the same lab product or an alternative is used in the 5 most similar protocols

For determination of the oligomeric state in solution, 50 µL of pure TtEst2 at 2 mg·mL⁻¹ was injected into a Superdex 75 Increase 10/300 GL (GE Healthcare, Velizy-Villacoublay, France) developed in 20 mM HEPES pH 7.5, 150 mM NaCl at a flow rate of 0.5 mL.min⁻¹ on an AKTA purified (GE Healthcare, Velizy-Villacoublay, France) multi-angle laser light scattering was measured in-line using a 8 angles DAWN^® Heleos^® II detector (Wyatt Technologies Corp., Toulouse, France) and a 663 nm laser light, and coupled to a Optilab^® T-rEx differential refractometer (Wyatt Technologies Corp., Toulouse, France) for refractive index measurements. Data were analyzed with Astra^® v6.1 software (Wyatt Technologies Corp., Toulouse, France) using a refractive index increment (

\frac{d n}{d c}

) of 0.190 mL·g⁻¹, a mean value usually accepted for proteins [29 (link)].

Bzdrenga J., Trenet E., Chantegreil F., Bernal K., Nachon F, & Brazzolotto X. (2021). A Thermophilic Bacterial Esterase for Scavenging Nerve Agents: A Kinetic, Biophysical and Structural Study. Molecules, 26(3), 657.

+ Open protocol

+ Expand

Oligomerization State Determination of hBChE-7

Check if the same lab product or an alternative is used in the 5 most similar protocols

For oligomerization state determination, 50 µL of pure hBChE-7 at a concentration of 1 mg mL⁻¹ was injected onto a Superdex-200 Increase 10/300 column (GE Healthcare, Vélizy-Villacoublay, France) developed in 20 mM Tris pH 8.0, 150 mM NaCl at a flow rate of 0.5 mL min⁻¹ on an AKTA purifier (GE Healthcare, Vélizy-Villacoublay, France). Multi-angle laser light scattering was recorded in-line with an 8 angles DAWN^® Heleos^® II detector (Wyatt Technologies Corp., Toulouse, France) using a 663 nm laser light and refractive index measurements were recorded on an Optilab^® T-rEx differential refractometer (Wyatt Technologies Corp., Toulouse, France). Data were treated using the Astra^® v6.1 software (Wyatt Technologies Corp., Toulouse, France), and the average molecular weight was determined using a differential index of refraction (

\frac{d n}{d c}

) value of 0.185.

Brazzolotto X., Igert A., Guillon V., Santoni G, & Nachon F. (2017). Bacterial Expression of Human Butyrylcholinesterase as a Tool for Nerve Agent Bioscavengers Development. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(11), 1828.

+ Open protocol

+ Expand

Characterization of BRSK Oligomeric State

Check if the same lab product or an alternative is used in the 5 most similar protocols

The oligomeric state of recombinant BRSKs was characterized by in-line Size Exclusion Chromatography-Multi-Angle Laser Light Scattering (SEC-MALS). Purified BRSK proteins (1 mg mL⁻¹) were applied directly to a HiLoad 16/60 Superdex 200 attached to an ÄKTA pure fast protein liquid chromatography (FPLC) system equilibrated in 10 mM Tris-HCl pH 7.4, 150 mM NaCl at a flow rate of 0.7 mL min⁻¹. Eluted protein was detected by a MALLS detector and a differential refractive index (DRI) detector (DAWN HELEOS-II and Optilab TrEX; Wyatt Technology, Santa Barbara, CA, USA). Data was analyzed using ASTRA v6.1 software (WYATT). The system was calibrated using BSA prior to data collection with BRSK1/2 proteins.

Bendzunas G.N., Byrne D.P., Shrestha S., Daly L.A., Oswald S.O., Katiyar S., Venkat A., Yeung W., Eyers C.E., Eyers P.A, & Kannan N. (2024). Redox Regulation of Brain Selective Kinases BRSK1/2: Implications for Dynamic Control of the Eukaryotic AMPK family through Cys-based mechanisms. bioRxiv.

+ Open protocol

+ Expand

SEC-MALS Analysis of Protein Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

Samples analyzed by SEC-MALS were buffer exchanged by dialysis into SEC-MALS running buffer containing 20 mM HEPES (pH 7.6), 200 mM NaCl, 5% glycerol, 0.5mM TCEP, and 0.05% azide. Samples were applied to a Superose6 10/300 GL (Sigma-Aldrich) column and in-line measurements were recorded for ultraviolet absorbance at 280 nm (using Agilent 1100 series UV detector, Agilent Technologies), static light scattering (DAWN HELEOS 8+light scattering detector, Wyatt Technology), and differential refractive index (Agilent 1200 series refractive index detector, Agilent Technologies) were recorded. System calibration was performed on a BSA standard (Sigma-Aldrich), first resuspended, then buffer exchanged into the SEC-MALS running buffer. SEC-MALS data was analyzed with ASTRA v6.1 software (Wyatt Technology).

Mason A.C., & Wente S.R. (2020). Functions of Gle1 are governed by two distinct modes of self-association.

+ Open protocol

+ Expand

Size Exclusion Chromatography and MALS Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Size exclusion chromatography and multiangle light scattering (SEC‐MALS) experiments were performed on a Dionex Ultimate 3000 HPLC system with an inline Wyatt miniDAWN TREOS MALS detector and Optilab T‐rEX refractive index detector. In addition, the elution profile of the protein was monitored with UV 280 attached to the Dionex system. For size exclusion chromatography, Superdex™ 200 Increase 10/300 GL column (GE Healthcare LifeSciences) was used. Fifty microliter of the purified UFL1/UFBP1 stored in buffer containing 25 mM Tris–pH 8.0, 300 mM NaCl and 2 mM DTT was loaded into the SEC column with Dionex autoloader at a concentration of 4 mg/ml and a flow rate of 0.3 ml/min was maintained throughout the experiment. Molar masses spanning elution peaks were calculated using ASTRA v6.0.0.108 (Wyatt).

Peter J.J., Magnussen H.M., DaRosa P.A., Millrine D., Matthews S.P., Lamoliatte F., Sundaramoorthy R., Kopito R.R, & Kulathu Y. (2022). A non‐canonical scaffold‐type E3 ligase complex mediates protein UFMylation. The EMBO Journal, 41(21), e111015.

+ Open protocol

+ Expand

Analyzing Phosphoprotein Interactions via SEC-MALS

Check if the same lab product or an alternative is used in the 5 most similar protocols

Size-exclusion chromatography and multiangle light scattering (SEC–MALS) experiments were performed on a Dionex Ultimate 3000 HPLC system with an inline Wyatt miniDAWN TREOS MALS detector and Optilab T-rEX refractive index detector. SEC–MALS experiments were performed on a Superdex S75 CL 10/300 (GE Healthcare) column in buffer containing 50 mM HEPES with 100 mM NaCl at pH 7.5. Phosphoubiquitin (140 μM) and phosphoparkin proteins (20 μM) were incubated for 30 min before injections. Molar masses spanning elution peaks were calculated using ASTRA v6.0.0.108 (Wyatt). For the size-exclusion analysis with UbcH7 or UbcH7∼Ub, phosphoparkin proteins (20 μM) were incubated with phosphoubiquitin (40 μM) and either UbcH7 (40 μM) or UbcH7∼Ub (40 μM) for 30 min prior to injections.

Kumar A., Aguirre J.D., Condos T.E., Martinez-Torres R.J., Chaugule V.K., Toth R., Sundaramoorthy R., Mercier P., Knebel A., Spratt D.E., Barber K.R., Shaw G.S, & Walden H. (2015). Disruption of the autoinhibited state primes the E3 ligase parkin for activation and catalysis. The EMBO Journal, 34(20), 2506-2521.

+ Open protocol

+ Expand

SEC-MALS Analysis of Protein Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

Size exclusion chromatography and multi angle light scattering (SEC–MALS) experiments were performed on a Dionex Ultimate 3000 HPLC system with an inline Wyatt miniDAWN TREOS MALS detector and Optilab T-rEX refractive index detector. Initially SEC–MALS experiments were performed on a MAbPac SEC–1 (Dionex) column (Figure 1) and due to column degradation subsequent experiments were performed on a Superdex S200 CL 10/300 (GE Healthcare) column (Figures 5 and 7). Buffer conditions were 20 mM HEPES-KOH pH 7.5 with sodium chloride concentrations as stated in the text. Molar masses spanning elution peaks were calculated ASTRA v6.0.0.108 (Wyatt).

Bowman A., Hammond C.M., Stirling A., Ward R., Shang W., El-Mkami H., Robinson D.A., Svergun D.I., Norman D.G, & Owen-Hughes T. (2014). The histone chaperones Vps75 and Nap1 form ring-like, tetrameric structures in solution. Nucleic Acids Research, 42(9), 6038-6051.

+ Open protocol

+ Expand

SEC-MALS Analysis of UFL1/UFBP1 Protein

Check if the same lab product or an alternative is used in the 5 most similar protocols

Size exclusion chromatography and multi angle light scattering (SEC-MALS) experiments were performed on a Dionex Ultimate 3000 HPLC system with an inline Wyatt miniDAWN TREOS MALS detector and Optilab T-rEX refractive index detector.
In addition, the elution profile of the protein was monitored with UV 280 attached to the Dionex system. For size exclusion chromatography, Superdex 200 10/300 gl column (GE Healthcare LifeSciences) was used. 50 µl of the purified UFL1/UFBP1 stored in buffer containing 25 mM Tris pH 8.0, 300 mM NaCl, 2 mM DTT was loaded into the SEC column with Dionex auto loader at a concentration of 4 mg/ml and a flow rate of 0.3 ml/min was maintained throughout the experiment. Molar masses spanning elution peaks were calculated using ASTRA v6.0.0.108 (Wyatt).

Peter J., Magnussen H., DaRosa P.A., Millrine D., Matthews S.P., Lamoliatte F., Sundaramoorthy R., Kopito R.R., & Kulathu Y. (2022). Non canonical scaffold-type ligase complex mediates protein UFMylation.

+ Open protocol

+ Expand

SEC-MALS Analysis of Purified Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

For size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), 100 μL purified proteins at 2–5 mg/mL were injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in gel filtration buffer. Light scattering and refractive index profiles were collected by miniDAWN TREOS and Optilab T-rEX detectors (Wyatt Technology), respectively, and molecular weight was calculated using ASTRA v. 6 software (Wyatt Technology).

Ye Q., West A.M., Silletti S, & Corbett K.D. (2020). Architecture and self-assembly of the SARS-CoV-2 nucleocapsid protein. bioRxiv.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!