Routine metabolic rate was measured as the rate of CO2 produced by groups of 20 larvae of the same instar and strain using a flow-through respirometry system (Sable Systems International, Henderson, NV) (Hoekstra et al. 2013 (link)). Groups of larvae were collected onto the cap of 1.7 ml tube containing 0.5 ml of fly medium and placed inside one of four respirometry chambers that were housed in a temperature-controlled cabinet (Tritech Research, Los Angeles, CA) maintained at 25°. Between 8 and 13 biological replicates for each strain and instar were randomized across chambers and respirometry runs, during which each group of larvae was sampled for CO2 production for two 10-min periods. CO2 that accumulated in the chambers as a result of larval metabolism was detected using an infrared CO2 analyzer (Li-Cor 7000 CO2/H2O Analyzer; LI-COR, Lincoln, NE). was calculated from the mean fractional increase in CO2 at a constant air-flow rate of 100 ml/min over a 10-min time interval for each replicate after baseline-drift correction. The wet weight of the group of larvae was recorded using a Cubis microbalance (Sartorius AG, Göttingen, Germany) at the beginning of each respirometry run.
Cubis microbalance
Manufactured by Sartorius
Sourced in Germany
The Cubis microbalance is a precision weighing instrument designed for laboratory applications. It offers high-accuracy measurements with a readability of up to 0.1 micrograms. The Cubis microbalance is suitable for a wide range of weighing tasks that require exceptional precision and repeatability.
Automatically generated - may contain errors
4 protocols using cubis microbalance
1
Measuring Larval Metabolic Rates
2
Nonwoven Thermal Diffusivity Characterization
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The samples’ height for cross-plane thermal diffusivity characterization was measured via laser microscopy (LEXT OLS5000, Olympus IMS). Cylinders of 10 mm in diameter were cut from the nonwovens. Individual samples were put on the microscope stage. To ensure a flat contact, a glass slide was put on either side of the sample, leaving an unobstructed strip of about 5 mm in the center of the sample. A height image was acquired, and the average height of the sample was determined.
The mass of the samples was determined with a microscale (Cubis Micro Balance, Sartorius Lab Instruments GmbH). The volume was calculated from the known area (A = πr2 ≈ 78.5 mm2) and the measured height. The measurement was repeated for nine samples for each OSZ content. The given density is the average of all samples with the standard deviation being the error.
The mass of the samples was determined with a microscale (Cubis Micro Balance, Sartorius Lab Instruments GmbH). The volume was calculated from the known area (A = πr2 ≈ 78.5 mm2) and the measured height. The measurement was repeated for nine samples for each OSZ content. The given density is the average of all samples with the standard deviation being the error.
3
Fly Weight Measurement Protocol
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Sets of ~30 five day old flies were kept in a 0.6 μl tube and weighed using Sartorius Cubis® Micro Balance. The average weight of each fly was calculated. Independent biological replicates of this experiment were performed, the number of replicates is available in the figure legends.
4
Resistance of Car Tire Particles to H2O2
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Prior to the purification
of the samples, the resistance of car tire
TP to 30% H2O2 was tested. For this, 80 car
tire TP cut from the scrapped sample were distributed in eight porcelain
cups and dried in an oven at 40 °C for 72h. The mean dry weight
of the particles from each cup (n = 10) was measured
with a Cubis Micro balance (Sartorius, Germany). Pictures of each
particle were taken with a CMEX camera (Euromex, The Netherlands)
under an Olympus SZX10 stereomicroscope and the mean particle area
(n = 10) was measured using ImageJ Software. Four
groups of 10 particles were added to glass beakers containing 30%
H2O2 and the other four were added to glass
beakers containing Milli-Q water. All glass beakers were placed in
a New Brunswick Scientific G25 shaking incubator at 45 °C and
80 rpm for 24 h. After this period, all particles were flushed with
water and dried in an oven at 40 °C for 72h. Finally, the mean
weight of the particles was measured again and new pictures were taken
to calculate the mean particle area.
of the samples, the resistance of car tire
TP to 30% H2O2 was tested. For this, 80 car
tire TP cut from the scrapped sample were distributed in eight porcelain
cups and dried in an oven at 40 °C for 72h. The mean dry weight
of the particles from each cup (n = 10) was measured
with a Cubis Micro balance (Sartorius, Germany). Pictures of each
particle were taken with a CMEX camera (Euromex, The Netherlands)
under an Olympus SZX10 stereomicroscope and the mean particle area
(n = 10) was measured using ImageJ Software. Four
groups of 10 particles were added to glass beakers containing 30%
H2O2 and the other four were added to glass
beakers containing Milli-Q water. All glass beakers were placed in
a New Brunswick Scientific G25 shaking incubator at 45 °C and
80 rpm for 24 h. After this period, all particles were flushed with
water and dried in an oven at 40 °C for 72h. Finally, the mean
weight of the particles was measured again and new pictures were taken
to calculate the mean particle area.
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