Ifn γ

Manufactured by Cusabio

Sourced in China, United States

IFN-γ is a protein that plays a crucial role in the immune system. It is produced by various cells, including T cells and natural killer cells, in response to antigenic stimulation. IFN-γ is involved in the regulation of immune and inflammatory responses, and it has been widely used in research and diagnostic applications.

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Lab products found in correlation

Tnf α, by Cusabio (6 mentions) Il 1β, by Cusabio (4 mentions) Interleukin 6 (il 6), by Cusabio (3 mentions) Il 10, by Cusabio (2 mentions) Multifilter f5 plate reader, by Molecular Devices (1 mentions) Interleukin 2 (il 2), by Cusabio (1 mentions) Yohimbine, by Merck Group (1 mentions) Ficoll pague plus, by GE Healthcare (1 mentions) Sb203580, by Merck Group (1 mentions) Rhil 2, by Thermo Fisher Scientific (1 mentions) Pd98059, by Merck Group (1 mentions) Rhil 4, by Thermo Fisher Scientific (1 mentions) Rhtnf α, by Thermo Fisher Scientific (1 mentions) Fitc conjugated dextran, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Ly294002, by Merck Group (1 mentions) I fabp, by Cusabio (1 mentions) Csb e04640r, by Cusabio (1 mentions) Csb e04727r, by Cusabio (1 mentions) Csb e08055r, by Cusabio (1 mentions)

12 protocols using ifn γ

Cytokine and Metabolite Quantification

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Concentrations of IFN-γ, IL-2, and KYN (all from CUSABIO) were analyzed by ELISA, according to the manufacturers’ instructions. Absorbance at 450 nm was measured on a Molecular Devices Multifilter F5 plate reader.

Shao J., Hou L., Liu J., Liu Y., Ning J., Zhao Q, & Zhang Y. (2021). Indoleamine 2,3-Dioxygenase 1 Inhibitor-Loaded Nanosheets Enhance CAR-T Cell Function in Esophageal Squamous Cell Carcinoma. Frontiers in Immunology, 12, 661357.

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Dendritic Cell Differentiation and Activation

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X-VIVO™ 15 serum-free specialty cell culture medium (catalog number: 04-418Q) was purchased from Lonza (Switzerland). DMSO (catalog number: d5879), yohimbine (catalog number: 731242), PD98059 (catalog number: P215), LY294002 (catalog number: L9908), SB203580 (catalog number: S8307) and FITC conjugated dextran (catalog number: FD40S) were purchased from Sigma (USA). Ficoll Pague PLUS (catalog number: 17-1440-03) was obtained from GE (USA), rh GM-CSF (recombinant human GM-CSF, catalog number: AF-300-03), rh IL-4 (catalog number: AF-200-04), rh TNF-α (catalog number: AF-300-01A) and rh IL-2 (catalog number: 200–02) were provided by Peprotech (Britain). PE or APC conjugated mouse-anti-human monoclonal antibody used for the detection of CD 11c, HLA-DR, CD86, CD80 were purchased by Biolegend (USA). Human IL-12 (catalog number: CSB-E04599h), IL-23 (catalog number: CSB-E08461h) and IFN-γ (catalog number: CSB-E04577h) Elisa kit were obtained by cusabio (China). The α2A rabbit-anti-human antibody (catalog number: ab65833) was purchased by Abcam (Britain). Reagent of dexmedetomidine was provided by Hengrui Medicine Company (China).

Chen G., Le Y., Zhou L., Gong L., Li X., Li Y., Liao Q., Duan K., Tong J, & Ouyang W. (2016). Dexmedetomidine Inhibits Maturation and Function of Human Cord Blood-Derived Dendritic Cells by Interfering with Synthesis and Secretion of IL-12 and IL-23. PLoS ONE, 11(4), e0153288.

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Quantifying Intestinal Biomarkers via ELISA

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ELISA kits were used to measure the concentrations of D-LA, ET (also known as lipopolysaccharide, LPS), L-FABP, i-FABP, DAO, TNF-α, IFN-γ, and IL-10 (CUSABIO, Wuhan, China) in intestinal lavage fluid, according to the manufacturer's instructions. The absorbance of each solution was determined at a wavelength of 450 nm. Assay sensitivities were determined as follows: 7.8 ng/ml for D-LA, 0.78 pg/ml for ET, 1.56 pg/ml for L-FABP, 0.312 ng/ml for i-FABP, 0.195 mIU/ml for DAO, 1.56 pg/ml for TNF-α, 0.156 pg/ml for IFN-γ, and 0.78 pg/ml for IL-10. Intra-assay and interassay coefficients were 8% and 10%, respectively, for D-LA, ET, L-FABP, i-FABP, DAO, TNF-α, IFN-γ, and IL-10.

Liu D.Y., Lou W.J., Zhang D.Y, & Sun S.Y. (2020). ROS Plays a Role in the Neonatal Rat Intestinal Barrier Damages Induced by Hyperoxia. BioMed Research International, 2020, 8819195.

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Cytokine and ONOO- Quantification

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After the specimens were placed at room temperature for 2 hours, they were centrifuged at 2–8°C at 1000 g for 15 minutes and the supernatant was taken for testing. The kit was as follows: IL-1β (China, CSB-E04640R), IL-6 (China, CSB-E08055R), IFN-γ (China, CSB-E04579R), and TGF-β1 (China, CSB-E04727R) were purchased from Cusabio Biotech Co. Ltd. ONOO⁻ (China, JL21035) was purchased from Jianglaibio Co. Ltd. Horseradish peroxidase-labeled avidin solution 100 μL was added to each well and incubated at 37°C for 1 hour. Substrate solution (90 μL) was added to each well in order, and color was developed at 37°C for 15–30 minutes. 50 μL of termination solution was added to each well in sequence to terminate the reaction. Within 5 minutes after the end of the reaction, the optical density (OD value) of each well was measured sequentially under an enzyme standard instrument (China, MB-530) at the wavelength of 450 nm.

Wan Y., Song M., Xie X., Chen Z., Gao Z., Wu X., Huang R, & Chen M. (2021). BMSCs Regulate Astrocytes through TSG-6 to Protect the Blood-Brain Barrier after Subarachnoid Hemorrhage. Mediators of Inflammation, 2021, 5522291.

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Serum Biomarkers for Immune Response

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Serum diamine oxidase (DAO) activity was measured using an assay kit (Jiancheng Institute of Bioengineering, Nanjing, China). Porcine enzyme-linked immunosorbent assay (ELISA) kits were used to measure IL-10, TNF-α, IFN-γ (CUSABIO Biotechnology Co., Ltd., Wuhan, China), and immunoglobulin (Ig) A (Solarbio Biotechnology Co., Ltd., Beijing, China). IgG, IgM, and superoxide dismutase (SOD) were determined by an autoanalyzer of Hitachi-7100 (Hitachi, Tokyo, Japan) using the corresponding kits (MedicalSystem Biotechnology Co., Ltd., Ningbo, China).

Li C., Wang Y., Zhao X., Li J., Wang H., Ren Y., Sun H., Zhu X., Song Q, & Wang J. (2024). Comparative Analysis of Intestinal Inflammation and Microbiota Dysbiosis of LPS-Challenged Piglets between Different Breeds. Animals : an Open Access Journal from MDPI, 14(5), 665.

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Serum Levels of Immune Biomarkers

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ELISA-based serum levels of interferon-γ (IFN-γ) (Cusabio, China), interleukin-17 (IL-17) (Mybiosource, USA), interleukin-10 (IL-10) (Cusabio, China), beta-defensin, and lipocalin-2 (LCN-2) (Mybiosource, USA) were determined.

Gad El-Karim D.R., El-Amrawi G, & Salama A.R. (2024). Clinicopathological studies on ulcerative lymphangitis in cattle: Alterations in serum inflammatory cytokines, anti-microbial, organs functions, and oxidative stress-related biomarkers. Open Veterinary Journal, 14(1), 25-31.

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Serum Cytokine Profiling in Rats

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After 14 days, serum was isolated from the blood of all rats. According to the manufacturer’s protocols, the concentrations of IL-17, IFN-γ, IL-β, IL-6, and TNF-α were measured using commercial enzyme-linked immunosorbent assay (ELISA) kits as per the instructions of manufacturers. The ELISA kits included IL-1β (Cusabio, Houston, TX, USA, CSB-E08055r), IL-6 (Cusabio, CSB-E04640r), IL-17 (Cusabio, CSB-E07451r), IFN-γ (Cusabio, CSB-E04579r), and TNF-α (Cusabio, CSB-E11987r).

Li J., Zhong J., Huang C., Guo J, & Wang B. (2021). Integration of traditional Chinese medicine and nibble debridement and dressing method reduces thrombosis and inflammatory response in the treatment of thromboangiitis obliterans. Annals of Translational Medicine, 9(18), 1413.

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Quantifying Inflammatory Factors in Lnc-DC Silencing

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The inflammatory factors in the cell culture supernatant after Lnc-DC silencing were detected by an ELISA kit with quantitative measurements, according to the manufacturer’s recommendations. The inflammatory factors included TNF-α, IL-1β, IL-6, IL-12, and IFN-γ (CUSABIO).

Zhuang L., Tian J., Zhang X., Wang H, & Huang C. (2018). Lnc-DC regulates cellular turnover and the HBV-induced immune response by TLR9/STAT3 signaling in dendritic cells. Cellular & Molecular Biology Letters, 23, 43.

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Microbial Transformation of Ginsenosides

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CK (No: S141001) was transformed from ginsenosides by microbial fermentation technology in Zhejiang Haizheng Pharmaceutical Co., Ltd (Taizhou, China). MTX (No.036140202) was purchased from Shanghai Xinyi Pharmaceutical Factory Co., Ltd (Shanghai, China). Rats were given CK or MTX with 0.5% sodium carboxymethylcellulose suspension, intragastric administration. BCG vaccine (20140804) was purchased in Chengdu Biological Products Co., Ltd (China). Lipopolysaccharides (LPS) and ConA were products of Sigma (USA). ELISA kits were purchased from ebioscience (USA), Raybiotech (USA) and CUSABIO (China), including TNF-α, IFN-γ, IL-1β, IL-2, IL-6, IL-17, PGE2, osteoprotegerin (OPG) and receptor activator of nuclear factor-κB ligand (RANKL). The Cell Counting Kit-8 was a product of Dojindo Molecular Technologies, Inc. (Tokyo, Japan).

Chen J., Wang W., Jiang M., Yang M, & Wei W. (2020). Combination therapy of ginsenoside compound K and methotrexate was efficient in elimination of anaemia and reduction of disease activity in adjuvant-induced arthritis rats. Pharmaceutical Biology, 58(1), 1140-1148.

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Goat Serum and Antibody Labeling Protocol

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Concentrated goat serum and Cy3-labeled Goat Anti-Rabbit IgG (cat. no. BA1034) were purchased from Wuhan Boster Biological Technology Co., Ltd.; Entranster^™-in vivo (cat. no. 18668-11-1) was purchased from Engreen Biosystem, Ltd.; Lipofectamine^® 2000 was purchased from Invitrogen (Thermo Fisher Scientific, Inc.); DNA marker was purchased from TransGen Biotech Co., Ltd.; RNAiso Plus, PrimeScript RT Reagent kit and SYBR Premix Ex Taq^™ II (Tli RNaseH Plus) were purchased from Takara Bio, Inc.; IL-5 (cat. no. E-EL-R0558c; Elabscience Biotechnology, Inc.), IL-33 (cat. no. CSB-E14077r; Cusabio Technology LLC) and IFN-γ (cat. no. CSB-E04579r; Cusabio Technology LLC) ELISA kits, and CD90 (cat. no. E-AB-70323) and CD45 (cat. no. E-AB-16319) antibodies were purchased from Elabscience Biotechnology, Inc.

Wu M., Yang J., Liu T., Xuan P., Bu B., Xu X, & Wu R. (2021). Effect of Src tyrosine kinase on a rat model of asthma. Experimental and Therapeutic Medicine, 23(2), 172.

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