Rl 95

Human EC cell lines Ishikawa, HEC-1A, RL-95, and AN3CA were obtained from Zhong Qiao Xin Zhou Biotechnology Co., Ltd (Shanghai, China), and KLE cells were purchased from Procell Life Science & Technology Co., Ltd (Wuhan, China). All the above cell lines were recently authenticated by using short tandem repeat (STR) analysis. Ishikawa cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; BI, Beit-Hamek, Israel). HEC-1A cells were cultured in McCoy’s 5A medium (Gibco, Carlsbad, USA) with 1% sodium pyruvate solution. RL-95 cells were cultured in DMEM-F12 (Gibco) with 1% sodium pyruvate solution and 5 μg/mL insulin. AN3CA cells were cultured in Minimal Essential Medium (MEM; Gibco) with 1% sodium pyruvate solution and 1% non-essential amino acid solution. KLE cells were cultured in DMEM-F12. All the media contained 10% fetal bovine serum (v/v) and 1% penicillin-streptomycin (v/v), and all the cells were cultured in an incubator containing 5% CO
₂ at 37°C. The compound TSI-01 was purchased from APExBio Company (Houston, USA).

Endometrial carcinoma cell lines HEC-1-B, RL-95 and KLE were purchased from ATCC and Ishikawa was purchased from Sigma-Aldrich. Cells were obtained directly from the cell banks, which perform cell line characterizations and passaged in the authors' laboratory for fewer than 6 months after resuscitation. HEC-1-B cell line was maintained in MEM (Gibco) supplemented with 10% fetal bovine serum (FBS), Ishikawa cell line was cultured in MEM (Gibco) supplemented with 5% FBS and KLE and RL-95 were maintained in DMEM (Gibco) supplemented with 10% FBS. All cell lines were maintained with supplementation of 2% penicillin/streptomycin and incubated in humidified chamber at 37°C in 5% CO₂ atmosphere.

Human endometrial cancer cell lines (Ishikawa, RL95 and HEC-1) and a human normal endometrial fibroblast cell line HESC were purchased from the American Type Culture Collection (ATCC, Manassas, VA). Dulbecco’s modified Eagle’s medium/nutrient mixture F-12 (DMEM/F12, Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific, Waltham, MA, USA) was used to culture Ishikawa, RL95, HEC-1, and HESC cells. All cell lines were maintained in a humidified 5% CO₂ incubator at 37°C.