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Dynabeads silane viral na

Manufactured by Thermo Fisher Scientific
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Sourced in Norway

Dynabeads SILANE viral NA are magnetic beads coated with silica-based material. They are designed for the isolation and purification of nucleic acids, such as DNA and RNA, from various sample types, including viral samples.

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Lab products found in correlation

Proteinase k, by Merck Group (2 mentions)

Close spelling variants of this product

Dynabeads SILANE Viral NA Kit Dynabeads

2 protocols using dynabeads silane viral na

1

Viral RNA Isolation using Dynabeads

Check if the same lab product or an alternative is used in the 5 most similar protocols
Viral RNA was isolated from samples using the Dynabeads SILANE viral NA kit (Invitrogen/Life Technologies AS, Oslo, Norway) employing the manufacturer’s recommended procedure. Briefly, 50 µl of Proteinase K (Sigma, P4850, 14 mg/ml) was first mixed with 200 µl of sample followed by mixing and incubation with 300 µl of lysis/binding buffer for 5 min at room temperature. 150 µl isopropyl alcohol (IPA) and 50 µl of Dynabeads were added to the mixture and incubated for 5 min on a roller. After capture of beads on a magnetic rack, 850 µl of Wash Buffer 1 were used to wash the beads two times. Then, these washes were repeated with Wash Buffer 2. After aspirating the wash buffer, the tube was left to air dry for 10 minutes to remove any residual alcohol and then RNA eluted with 50 µL of Elution Buffer.
Chen Z., Zhu H., Malamud D., Barber C., Ongagna Y.Y., Yasmin R., Modak S., Janal M.N., Abrams W.R, & Montagna R.A. (2016). A Rapid, Self-confirming Assay for HIV: Simultaneous Detection of Anti-HIV Antibodies and Viral RNA. Journal of AIDS & clinical research, 7(1), 540.
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2

Viral RNA Isolation using Dynabeads

Check if the same lab product or an alternative is used in the 5 most similar protocols
Viral RNA was isolated from samples using the Dynabeads SILANE viral NA kit (Invitrogen/Life Technologies AS, Oslo, Norway) employing the manufacturer’s recommended procedure. Briefly, 50 µl of Proteinase K (Sigma, P4850, 14 mg/ml) was first mixed with 200 µl of sample followed by mixing and incubation with 300 µl of lysis/binding buffer for 5 min at room temperature. 150 µl isopropyl alcohol (IPA) and 50 µl of Dynabeads were added to the mixture and incubated for 5 min on a roller. After capture of beads on a magnetic rack, 850 µl of Wash Buffer 1 were used to wash the beads two times. Then, these washes were repeated with Wash Buffer 2. After aspirating the wash buffer, the tube was left to air dry for 10 minutes to remove any residual alcohol and then RNA eluted with 50 µL of Elution Buffer.
Chen Z., Zhu H., Malamud D., Barber C., Ongagna Y.Y., Yasmin R., Modak S., Janal M.N., Abrams W.R, & Montagna R.A. (2016). A Rapid, Self-confirming Assay for HIV: Simultaneous Detection of Anti-HIV Antibodies and Viral RNA. Journal of AIDS & clinical research, 7(1), 540.
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+ Expand

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