Irdye 800cw goat anti mouse 926 32210

Manufactured by LI COR

Sourced in United States

The IRDye 800CW goat anti-mouse (926-32210) is a fluorescent-labeled secondary antibody that can be used for detection of mouse primary antibodies in Western blotting, immunocytochemistry, and other immunoassay applications. The IRDye 800CW fluorescent dye is conjugated to the goat anti-mouse IgG antibody, allowing for sensitive near-infrared fluorescent detection of target proteins.

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Lab products found in correlation

Mouse monoclonal igg2b, by Santa Cruz Biotechnology (1 mentions) Irdye 680rd, by LI COR (1 mentions) Ab41902, by Abcam (1 mentions) Ab28479, by Abcam (1 mentions) Rabbit anti histone h3, by Cell Signaling Technology (1 mentions) Dihydroethidium dhe, by Thermo Fisher Scientific (1 mentions) Rabbit anti akt, by Cell Signaling Technology (1 mentions) Nf κb p65 c22b4, by Cell Signaling Technology (1 mentions) Rabbit anti, by Merck Group (1 mentions) Rabbit anti p38 mitogen activated protein kinase p38 mapk, by Cell Signaling Technology (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Bicinchoninic acid protein assay kit, by Thermo Fisher Scientific (1 mentions) Irdye 800cw goat anti rabbit 926 32 211, by LI COR (1 mentions) Ripa reagent, by Beyotime (1 mentions) Mouse anti β actin ab8226, by Abcam (1 mentions) Phospho mapk family antibody sampler kit, by Cell Signaling Technology (1 mentions) Ab181602, by Abcam (1 mentions) Dpx mounting media, by Merck Group (1 mentions) Mn1020, by Santa Cruz Biotechnology (1 mentions) Irdye 680rd goat anti rabbit, by LI COR (1 mentions)

Close spelling variants of this product

IRDye 800CW Goat-Anti-Mouse IgG (926-32210) IRDye®800CW anti-mouse (926-32210) IRDye 800CW goat anti-mouse Anti-goat IRDye 800CW Anti-mouse IRDye 800CW Goat anti-mouse 800CW IRDye goat anti-mouse IRDye 800CW

5 protocols using irdye 800cw goat anti mouse 926 32210

Time-course of ERK1/2 Activation in β2AR-stimulated Cells

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The experimental details provided below for Western blotting experiments complies with BJP’s Guidelines for immunoblotting and immunohistochemistry (Alexander et al., 2018 (link)). HEK293 cells with endogenous β₂AR were stimulated with 10 μM β-agonist for 0, 2, 5, 10, 15, 20, 25, or 30 minutes. The cells were lysed in Laemmli buffer, and equal amounts of protein were analyzed by SDS PAGE and Western blot. The membranes were incubated overnight at 4 °C with anti-phospho-ERK 1/2 (P-p44/42 MAPK; rabbit mAB; Ref:4370S; Lot 31; 1:5000; Cell Signaling Technologies, Danvers, MA, USA) and anti-total ERK (ERK1/2 (C-9); sc-514302; Lot #I19.3; mouse monoclonal IgG_2B; 1:10000; Santa Cruz Biotechnology, Dallas, TX, USA) followed by IRDye conjugates (IRDye800CW, goat anti-Mouse, 926–32210, Lot C91210–09/ IRDye680RD, goat-anti-rabbit 926–68071, Lot D00115–06; 1:300; Li-Cor Biosciences, Lincoln, NE, USA) as secondary antibodies. Protein bands were detected with a LI-Cor Odyssey CLX far-infrared scanner (Li-Cor Biosciences, Lincoln, NE, USA) and the bands were quantified by densitometry with ImageJ v. 1.53 (RRID:SCR_003070) (Schneider et al., 2012 (link)).

De Pascali F., Ippolito M., Wolfe E., Komolov K.E., Hopfinger N., Lemenze D., Kim N., Armen R.S., An S.S., Scott C.P, & Benovic J.L. (2022). β-agonist profiling reveals biased signalling phenotypes for the β2-adrenergic receptor with possible implications for the treatment of asthma. British journal of pharmacology, 179(19), 4692-4708.

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Synthetic LXR Ligand GW3965 Protocol

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Synthetic LXR ligand 3-[3-[N-(2-Chloro-3-trifluoromethylbenzyl)-(2,2-diphenylethyl) amino] propyloxy] phenylacetic acid hydrochloride (GW3965) was kindly donated by Jon Collins (GlaxoSmithKline, Research Triangle Park, NC). Dihydroethidium (DHE) and TRIzol Reagent were from Life Technologies (Carlsbad, CA). Mouse monoclonal antibody against LXRα (ab41902) and rabbit polyclonal antibody against LXRβ (ab28479) were from Abcam (Cambridge, UK); rabbit anti-mouse nitrotyrosine antibody (06-284) was from Millipore (Billerica, MA); rabbit anti-cleaved caspase-3 (5A1E, #9664), rabbit anti-nuclear factor kappa-light-chain-enhancer of activated B cell p65 (NF-κB p65, C22B4; #4764), rabbit anti-Akt (#9272), rabbit anti-phospho-Akt (D9E, Ser473, #4060), rabbit anti-p38 mitogen-activated protein kinase (p38 MAPK, #9212), rabbit anti-phospho-p38 MAPK (D3F9, Thr180/Tyr182, #4511), rabbit anti-c-Jun N-terminal kinase (JNK, 56G8, #9258), rabbit anti-phospho-JNK (81E11, Thr183/Tyr185, #4668), rabbit anti-Histone H3 (#9715) and rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 14C10, #2118) were from Cell Signaling Technology (Beverly, MA). IRDye 800CW goat anti-mouse (926-32210) and anti-rabbit IgG (926-32211) secondary antibodies were from LI-COR Biosciences (Lincoln, NE).

He Q., Pu J., Yuan A., Yao T., Ying X., Zhao Y., Xu L., Tong H, & He B. (2014). Liver X receptor agonist treatment attenuates cardiac dysfunction in type 2 diabetic db/db mice. Cardiovascular Diabetology, 13, 149.

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Western Blot Analysis of Signaling Pathways

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Whole cell lysates were extracted using RIPA reagent (Beyotime, China) supplemented with protease inhibitors (Roche cOmplete ULTRA tablets), and protein concentrations determined using a Bicinchoninic Acid Protein assay Kit (Thermo Fisher, USA). Total protein aliquots (20 μg) were separated by SDS-PAGE, as described previously. Primary antibodies against p-EGFR (#3777S, CST), EGFR (#2085, CST), p-AKT (#4060, CST), AKT (#4821, CST), IFIT-1 (23247-1-AP, Proteintech), IFIT-3 (15201-1-AP, Proteintech), and β-actin (#4970, CST) were applied in this study. Secondary antibodies used were IRDye 800CW Goat anti-rabbit 926-32211 (Lot No. C90723-19, LI-COR) and IRDye 800CW Goat anti-mouse 926-32210 (Lot No. C90408-08, LI-COR).

Zan X., Li S., Wei S., Gao L., Zhao L., Yan X., Zhao Y., Shi J., Wang Y., Liu R., Zhang Y., Wan Y, & Zhou Y. (2022). COL8A1 Promotes NSCLC Progression Through IFIT1/IFIT3-Mediated EGFR Activation. Frontiers in Oncology, 12, 707525.

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Lipid Extraction and LC-MS Analysis of Bioactive Lipids

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Ammonium formate (516961), ammonium acetate (372331), ammonium hydroxide (338818) and lipopolysaccharides from E. coli 0111:B4 (L4391) were from Sigma-Aldrich. Formic acid (06440) was from Fluka. Water (365-4), methanol (230-4), isopropanol (323-4), acetonitrile (017-4) and chloroform (049-1L) for lipid extraction and LC–MS were from Honeywell Burdick & Jackson. C4 silica packing material for precolumn was from Western Analytical Products (214TPB1520) and C18 silica packing material for precolumn was from VWR (53501-270). 6-keto-PGF_1α (10007219), 6, 15-diketo-13, 14-dihydro-PGF_1α (15270), 20-hydroxy-PGF_2α (16950), thromboxane B₂ (19030), 19(R)-hydroxy-PGF_2α (16910) and 19(R)-hydroxy-PGE₁ (13910) were from Cayman Chemical. Mouse anti-COX-1 (ab695), rabbit anti-COX-2 (ab15191) and mouse anti-β-actin (ab8226) antibodies were from Abcam. NF-κB Pathway Sampler Kit (9936), MAPK Family Antibody Sampler Kit (9926) and phospho-MAPK Family Antibody Sampler Kit (9910) were from Cell Signaling Technology. Rabbit anti-BAX (sc-493) and rabbit anti-JNK1/3 (sc-474) antibodies were from Santa Cruz Biotechnology. IRDye 680LT donkey anti-rabbit (926-68023), IRDye 800CW goat anti-rabbit (926-32211) and IRDye 800CW goat anti-mouse (926-32210) antibodies were from LI-COR Biosciences.

Zhang T., Walensky L.D, & Saghatelian A. (2015). A non-apoptotic role for BAX and BAK in eicosanoid metabolism. ACS chemical biology, 10(6), 1398-1403.

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Immunohistochemical Analysis of Phosphorylated Tau

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Phosphatase inhibitor cocktail IV (ab201115) and antibody against GAPDH (ab181602) were purchased from Abcam. Primary antibodies against phosphorylated tau at Ser396 (PHF13; sc-32275), Ser202/Thr205 (AT8; MN1020) and Thr212/Ser214 (AT100; MN1060) were procured from Santa Cruz Biotechnology and Thermo Scientific, respectively. Secondary antibodies IRDye^® 800 CW goat anti-mouse (926-32210) and IRDye^® 680 RD goat anti-rabbit (926-68071) were sourced from LI-COR Biosciences. Immunohistochemical biotinylated link and streptavidin/HRP complex solutions (LSAB2 System-HRP Kit, K0675) were from Dako. Protease inhibitors pepstatin A (P5318), leupeptin hydrochloride (L9783) and phenylmethanesulfonylfluoride (PMSF; P7626), 3,3’-diaminobenzidine and H₂O₂/urea tablets (D4293-50SET) and DPX mounting media (44581) were from Sigma–Aldrich. Other reagents used in this study were of analytical grade and procured from either Thermo Fisher Scientific or Sigma–Aldrich.

Ahmad F., Mein H., Jing Y., Zhang H, & Liu P. (2021). Behavioural Functions and Cerebral Blood Flow in a P301S Tauopathy Mouse Model: A Time-Course Study. International Journal of Molecular Sciences, 22(18), 9727.

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