The PBM and PBM-R tumor cells were transplanted orthotopically into 6–8 weeks old female syngeneic FVB/NJ mice to generated tumors for drug evaluation. Tumor-bearing mice were randomized into control and treatment groups according to the luminescent intensity as previously described.12 (link) Olaparib (AZD2281) was administered daily by i.p. injection at a dose of 50 mg/kg body weight. MSA-2 was prepared by diluting 50 mg/mL stock in DMSO (Dimethyl sulfoxide) with phosphate-buffered saline (pH 8.0) and administered every other day (three times a week, 2 weeks on followed by 1 week off) by i.p. injection at a dose of 25 mg/kg body weight. The endpoints were determined by tumor burden and ascites. For the PDX in vivo experiments, about 3×106 PDX tumor cells were mixed with 3×106 human bone marrow mononuclear cells in serum-free DMEM/F12 medium containing 50% Matrigel (CAT# 70001, STEMCELL Technology) and i.p. transplanted into 4-week-old female NOD/SCID IL2Rgnull mice (NSG, The Jackson Laboratory). About 3 weeks after injection, PDX-bearing mice were randomized into four groups according to the luminescent intensity and treated with vehicle control, olaparib, MSA-2 and olaparib in combination with MSA-2 using the same dosing and schedule that have been described above. Ascites was harvested for analysis after 3 weeks’ treatment.
Il2rgnull mice
Manufactured by Jackson ImmunoResearch
IL2Rgnull mice are a genetically engineered mouse model that lacks the interleukin-2 receptor gamma (IL2Rg) gene. This gene is essential for the development and function of natural killer cells, T cells, and B cells. The IL2Rgnull mice are a valuable tool for studying the role of the IL2Rg gene in immune system development and function.
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Lab products found in correlation
3 protocols using il2rgnull mice
1
Preclinical Evaluation of Olaparib and MSA-2 in Tumor Xenografts
2
Xenograft Generation from Pediatric Tumor PDXs
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All experiments were conducted per protocols and conditions approved by the University of Texas MD Anderson Cancer Center (MDACC; Houston, TX) Institutional Animal Care and Use Committee (eACUF Protocols #00000712-RN02). Male NOD (SCID)-IL-2Rgnull mice (The Jackson Laboratory; Farmington, CT) were subcutaneously injected with PDX explants (2 mm) to generate xenografts. All mice were maintained under barrier conditions and treated using protocols approved by The University of Texas MD Anderson Cancer Center’s Institutional Animal Care and Use Committee. SA98 (full id: MDA-SA98-TIS02), OS1, and OS31, are PDX lines maintained by the Pediatric Solid Tumors Comprehensive Data Resource Core [13 (link)]. DSRCT and ES PDX lines were generated from the Sarcoma Tissue Bank at MD Anderson Cancer Center and maintained by the Ludwig lab. Once their tumors reached a volume of 150 mm [3 (link)], tumors were explanted and a portion was flash-frozen for snRNA-seq, while the remainder underwent dissociation.
3
Muscle Defect Repair in Mice
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All animal studies were approved by the Institutional Animal Care and Use Committee at the Veterans Affairs Palo Alto Health Care System. VML was induced in immunocompromised NOD-scid IL2Rgnull mice (male, 8 weeks old, Jackson Laboratory) by surgical excision of 20% of the tibialis anterior (TA) muscle bilaterally, excising a segment that was 7 mm × 2 mm × 3 mm.14 (link),39 Immediately afterwards, animal were randomized to receive one of the following treatment groups per legat the site of the muscle defect: (1) no treatment (n = 4); (2) randomly oriented scaffold aggregate (n = 6); (3) aligned scaffold aggregate (n = 6); or (4) commercial decellularized scaffolds (Gentrix, Acell Corporation, n = 4). Each scaffold aggregate was composed of eight scaffold strips organized as a parallel bundle with dimensions of 9 mm × 2 mm × 3 mm. The decellularized scaffold served as a basis for comparison to nanofibrillar scaffolds. Following scaffold transplantation, animals were allowed to recover in traditional housing cages for 21 days before being euthanized for histological analysis.
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