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Fatty acid standard fa 17 1

Manufactured by Nu-Chek Prep

Fatty acid standard FA(17:1) is a reference material used for the identification and quantification of fatty acids in analytical applications. It contains the saturated fatty acid heptadecanoic acid (17:1), which can serve as a calibration standard or internal standard in analytical techniques such as gas chromatography (GC) and liquid chromatography (LC).

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2 protocols using fatty acid standard fa 17 1

1

Standards for Lipid Profiling Analysis

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The following internal standards were obtained from Avanti Polar Lipids (Alabaster, AL): sphingolipid mix (SM) II, phosphatidylethanolamine PE (14:0/14:0), phosphatidylcholine PC (14:0/14:0), phosphatidic acid PA (14:0/14:0), phosphatidylserine PS (14:0/14:0), phosphatidylinositol PI (17:0/20:4), and lysophosphatidylcholine LPC (17:1). Fatty acid standard FA(17:1) from Nu-Chek Prep (Elysian, MN). UPLC-grade solvents acetonitrile, water, and isopropanol were purchased from Fisher Scientific (Hanover Park, IL). Arachidonic acid, azelaic acid and S-(5′-adenosyl)-L-homocysteine, was purchased from Sigma-Aldrich (St Louis, MO). Adrenic acid was purchased from Santa Cruz (Dallas, Texas). The MSMS spectra from METLIN8 (link) and LipidMaps databases were used as reference spectra for comparison purposes.
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2

Comprehensive Lipidomic and Metabolomic Analysis

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The following fatty acids and lipid standards
were purchased from Avanti Polar lipids (Alabaster, AL): sphingolipid
mix (SM) II, phosphatidylethanolamine PE (14:0/14:0), phosphatidylcholine
PC (14:0/14:0), phosphatidic acid PA (14:0/14:0), phosphatidylserine
PS (14:0/14:0), phosphatidylinositol PI (17:0/20:4), and lysophosphatidylcholine
LPC (17:1). Prostaglandin standard and leukotriene were purchased
from Cayman Chemical (Ann Arbor, MI), and fatty acid standard FA(17:1)
was purchased from Nu-Chek Prep (Elysian, MN). Debrisoquine sulfate,
4-nitrobenzoic acid (4-NBA), and UPLC-grade solvents such as acetonitrile,
water, and isopropanol were purchased from Fisher Scientific (Hanover
Park, IL). Glucose, riboflavin, arachidonic acid, linoleic acid, oleic
acid, palmitic acid, hippuric acid, nicotinic acid, lactic acid, uridine,
taurine, α-ketobutyric acid, uric acid, hydroxyphenylpyruvic
acid, acetylcarnitine, and carnitine were purchased from Sigma-Aldrich
(Seelze, Germany). The tandem MS spectrum of dityrosine provided by
Hanft et al. was used as a validation reference spectrum.8 (link) In addition, the MS/MS spectra provided by Scripps
Center for Metabolomics, METLIN, (La Jolla, CA) were used as reference
spectra for hydroquinone, inositol, gentisic acid, and dihydrolipoamide.
METLIN was also used to putatively identify the ion at m/z of 337.1048 as the sodium adduct of 7,8-dihydropteroic
acid.
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