Sophorose

Manufactured by Merck Group

Sourced in United States

Sophorose is a disaccharide composed of two glucose units linked by a β-1,2 glycosidic bond. It is a naturally occurring compound found in various organisms, including fungi and bacteria. Sophorose functions as an activator of cellulase enzymes, which play a role in the degradation of cellulose.

Automatically generated - may contain errors

Lab products found in correlation

8 protocols using sophorose

R. stolonifer TP-02 Isolation and Materials

Check if the same lab product or an alternative is used in the 5 most similar protocols

R. stolonifer TP-02 was isolated in our laboratory and stored in China General Microbiological Culture Collection Center (CGMCC No. 11119). Sophorose, ATP, and UDPG were purchased from Sigma-Aldrich.

Zhang Y., Tang B, & Du G. (2017). Self-induction system for cellulase production by cellobiose produced from glucose in Rhizopus stolonifer. Scientific Reports, 7, 10161.

+ Open protocol

+ Expand

Optimizing β-Glucosidase Production in Bacillus subtilis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Strain Escherichia coli JM109, T4DNA ligase, DNA polymerase PrimeSTAR ^®HS, In-Fusion HD Cloning Plus kit and restriction enzymes were purchased from Dalian BaoBio Co., Ltd. (Dalian, China). The expression host Bacillus subtilis WS11 and the expression vector pBSMμL3 of B. subtilis were constructed in the early works of our laboratory. Agarose gel DNA recovery kit, PCR purification kit and plasmid extraction kit were purchased from Beijing Tiangen Biochemical Technology Co., Ltd. (Beijing, China). The coding gene of β-glucosidase TsBgl1 and PCR primers were synthesized by Wuxi Tianlin Biotechnology Co., Ltd. (Wuxi, China). Ampicillin (Amp) and kanamycin (Kan) were purchased from Shanghai Jerry Co., Ltd. (Shanghai, China). The substrates 4-nitrophenyl-β-D-glucopyranoside (pNP-β-Glc), cellobiose, sophorose, gentiobiose and laminaribiose were purchased from Sigma-Aldrich (St. Louis, MO, USA). GOD glucose detection kit was purchased from Shanghai Rongsheng Biotechnology Co., Ltd. (Shanghai, China). Other conventional reagents are purchased from Sinopharmaceutical Group Chemical Reagent Co., Ltd. (Shanghai, China).

Xia W., Sheng L., Mu W., Shi Y, & Wu J. (2022). Enzymatic Preparation of Gentiooligosaccharides by a Thermophilic and Thermostable β-Glucosidase at a High Substrate Concentration. Foods, 11(3), 357.

+ Open protocol

+ Expand

Heterologous expression of a cellulase in Pichia pastoris

Check if the same lab product or an alternative is used in the 5 most similar protocols

H. insolens Y1 GCMCC 4573 was routinely cultured in the wheat bran medium45 (link). Escherichia coli Trans1-T1 and vector pEASY-T3 (TransGen, Beijing, China) were used for gene cloning. The gene expression vector and heterologous expression host were pPIC9 and P. pastoris GS115 (Invitrogen, Carlsbad, CA), respectively. The DNA purification kit, restriction endonucleases and LA Taq DNA polymerase were purchased from TaKaRa (Otsu, Japan). T4 DNA ligase and the total RNA isolation system kit were purchased from Promega (Madison, WI). The cDNA synthesis kit was purchased from TransGen. Barley β-glucan, Avicel, 4-nitrophenyl β-d-glucopyranoside (pNPG), 4-nitrophenyl β-d-xylopyranoside (pNPX), 4-nitrophenyl α-l-arabinofuranoside (pNPAf), 4-nitrophenyl α-d-galactopyranoside (pNPGal), 4-nitrophenyl α-l-arabinopyranoside (pNPAb), 4-nitrophenyl β-d-cellobioside (pNPC), disaccharides cellobiose, sophorose and gentibiose and soybean flavones daidzin, genistin and glycitin were all purchased from Sigma-Aldrich. Sodium carboxymethylcellulose (CMC-Na), laminarin and lichenin were obtained from Megazyme (Wicklow, Ireland). All other chemicals used were of analytical grade and commercially available.

Xia W., Bai Y., Cui Y., Xu X., Qian L., Shi P., Zhang W., Luo H., Zhan X, & Yao B. (2016). Functional diversity of family 3 β-glucosidases from thermophilic cellulolytic fungus Humicola insolens Y1. Scientific Reports, 6, 27062.

+ Open protocol

+ Expand

HPAEC-PAD for Enzymatic Reaction Products

Check if the same lab product or an alternative is used in the 5 most similar protocols

For detection of enzymatic reaction products, high-performance anion exchange column chromatography (HPAEC) with a pulsed amperometoric detector (PAD) equipped with a CarboPac PA10 guard column (4 × 50 mm) and a CarboPac PA10 analytical column (4 × 250 mm; Dionex Co.) was used. Enzymatic reaction was performed by incubation with equivalent volume of rAaBGL1 (20.0 nM) and each substrate in 20 mM sodium acetate buffer (pH 5.0) at 37°C. Reaction mixture was sampled at appropriate time, and added into equal volume of 0.2 M NaOH. Resultant mixtures were subjected to HPAEC-PAD using mobile phase of 100 mM NaOH with 10 mM sodium acetate. Glucose, cellobiose (Wako Pure Chemical Industries, Ltd.), cellotriose, cellotetraose, cellopentaose, laminaribiose, laminaritriose, laminaritetraose, laminaripentase (Megazyme), gentiobiose, sophorose (SIGMA-ALDRICH, Co.) were used as standards.

Baba Y., Sumitani J.I., Tani S, & Kawaguchi T. (2015). Characterization of Aspergillus aculeatus β-glucosidase 1 accelerating cellulose hydrolysis with Trichoderma cellulase system. AMB Express, 5, 3.

+ Open protocol

+ Expand

Enzymatic Substrate Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Restriction endonucleases, DNA ligase, and DNA polymerase were purchased from Takara Bio (Shiga, Japan). The QIAquick Kit was obtained from Qiagen (Hilden, Germany). 5-Bromo-4-chloro-3-indolyl-β-D-glucoside (X-glc) was purchased from Rose Scientific (Edmonton, AB, Canada). p-Nitrophenyl (pNP) α-D-galactopyranoside, pNP α-D-glucopyranoside, and pNP β-D-xylopyranoside were purchased from Nacalai (Kyoto, Japan). pNP α-D-mannopyranoside was purchased from Senn Chemicals (Zürich, Switzerland). The following chemicals were purchased from Sigma (St. Louis, MO, USA): avicel, pNP α-L-arabinofuranoside, pNP α-L-arabinopyranoside, pNP β-L-arabinopyranoside, pNP α-L-fucopyranoside, pNP β-D-fucopyranoside (pNPFuc), pNP β-D-galactopyranoside, pNP β-D-glucopyranoside (pNPGlc), pNP β-D-mannopyranoside, pNP N-acetyl-β-D-glucosaminide, pNP α-L-rhamnopyranoside, pNP α-D-xylopyranoside, and pNP β-D-cellobioside, sophorose, nigerose, maltose, isomaltose, lactose, and salicin. Cello-origosaccharides and laminaribiose were purchased from Seikagaku Kogyo (Tokyo, Japan). Gentiobiose was purchased from Tokyo Chemical Industry (Tokyo, Japan).

Uchiyama T., Yaoi K, & Miyazaki K. (2015). Glucose-tolerant β-glucosidase retrieved from a Kusaya gravy metagenome. Frontiers in Microbiology, 6, 548.

+ Open protocol

+ Expand

Glycan Derivatization with Me-FRAGS Reagent

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Maltose, cellobiose, lactose, melibiose, isoMaltose, nigerose, kojibiose, sophorose, gentobiose, maltotetraose, and glucose tetrasaccharide were purchased from Sigma-Aldrich (St. Louis, MO, USA). All solvents were of HPLC grade and were purchased from EMD Merck (Gibbstown, NJ, USA). All other chemicals for the synthesis of Me-FRAGS reagent were purchased from Sigma-Aldrich (St. Louis, MO, USA). The synthesis of the Me-FRAGS reagent and the glycan derivatization were achieved according to previously reported procedures.^{58 (link)} Me-FRAGS selectively couples with glycan epimers via derivatization at the unique reducing terminus in water in the presence of 10% acetic acid, which takes several hours at 50–70 °C. The introduction of the methyl group on the pyridine moiety was achieved by allowing the FRAGS-derivatized glycans to react with iodomethane in acetonitrile at room temperature for 6 h. The samples were dried via a vacuum concentrator and redissolved in 50/50 methanol/water (v/v).

Murtada R., Fabijanczuk K., Gaspar K., Dong X., Alzarieni K.Z., Calix K., Manriquez E., Bakestani R.M., Kenttämaa H.I, & Gao J. (2020). Free-Radical-Mediated Glycan Isomer Differentiation. Analytical chemistry, 92(20), 13794-13802.

+ Open protocol

+ Expand

Cloning and Expression of Glycoside Hydrolases

Check if the same lab product or an alternative is used in the 5 most similar protocols

Chemicals, plasmids, and culture media Laminaribiose, sophorose, p-nitrophenol (pNP), and p-nitrophenol-β-D -glucoside(pNPG) were purchased from Sigma-Aldrich Corporation (St. Louis, MO, USA). Kanamycin and isopropyl-1-thio-β-Dgalactopyranoside (IPTG) were purchased from Gen-view Scienti c Inc. (El Monte, CA, USA). The KOD-Plus-Mutagenesis Kit was purchased from Toyobo Co., Ltd. (Osaka, Japan). All other chemicals were from Sangon Biotech Co., Ltd. (Shanghai, China). Plasmid pET-32a was purchased from Invitrogen (Carlsbad, CA, USA). Restriction enzymes and T4 DNA ligase were purchased from Thermo Fisher Scienti c (Shanghai, China). Primers were synthesized by Sangon Biotech Co., Ltd. Escherichia coli DH5α and E. coli BL21 (DE3) was purchased from TransGen Biotech (Beijing, China).

Niu K., Liu Z., Feng Y., Gao T., Wang Z., Zhang P., Du Z., Gao D., & Fang X. (2020). A novel strategy for efficient disaccharides synthesis from glucose by β-glucosidase.

+ Open protocol

+ Expand

Recombinant Protein Expression Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Laminaribiose, sophorose, p-nitrophenol (pNP), and p-nitrophenol-β-D -glucoside(pNPG) were purchased from Sigma-Aldrich Corporation (St. Louis, MO, USA). Kanamycin and isopropyl-1-thio-β-D -galactopyranoside (IPTG) were purchased from Gen-view Scientific Inc. (El Monte, CA, USA). The KOD-Plus-Mutagenesis Kit was purchased from Toyobo Co., Ltd. (Osaka, Japan). All other chemicals were from Sangon Biotech Co., Ltd.
(Shanghai, China). Plasmid pET-32a was purchased from Invitrogen (Carlsbad, CA, USA). Restriction enzymes and T4 DNA ligase were purchased from Thermo Fisher Scientific (Shanghai, China). Primers were synthesized by Sangon Biotech Co., Ltd. Escherichia coli DH5α and E. coli BL21 (DE3) was purchased from TransGen Biotech (Beijing, China).

Niu K., Liu Z., Feng Y., Gao T., Wang Z., Zhang P., Du Z., Gao D., & Fang X. (2020). A novel strategy for efficient disaccharides synthesis from glucose by β-glucosidase.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!