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Gm09503

Manufactured by Coriell Institute for Medical Research
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GM09503 is a cell line derived from peripheral blood lymphocytes of a healthy male individual. It is a commonly used reference cell line for research purposes.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Gm25313, by Coriell Institute for Medical Research (2 mentions) Hesc qualified matrigel, by Corning (2 mentions) Human pluripotent stem cell functional identification kit, by R&D Systems (2 mentions) Stemdiff cardiomyocyte differentiation kit, by STEMCELL (2 mentions) Gm04569, by Coriell Institute for Medical Research (2 mentions) Gm05089, by Coriell Institute for Medical Research (2 mentions) Gm02298, by Coriell Institute for Medical Research (2 mentions) Gm04981, by Coriell Institute for Medical Research (2 mentions) Mtesr1 medium, by STEMCELL (2 mentions) L glutamine, by Corning (1 mentions) Sw1353, by American Type Culture Collection (1 mentions) Penicillin streptomycin, by Corning (1 mentions) Gm00038, by Coriell Institute for Medical Research (1 mentions) Gm01651, by Coriell Institute for Medical Research (1 mentions) Gm01652, by Coriell Institute for Medical Research (1 mentions) Pen strep, by Thermo Fisher Scientific (1 mentions) Mem non essential amino acid, by Thermo Fisher Scientific (1 mentions) Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions) Gm05565, by Coriell Institute for Medical Research (1 mentions)

5 protocols using gm09503

1

Fibroblast Culturing Protocol for Research

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Primary fibroblasts derived from healthy controls (GM00038 and GM09503) were obtained from Coriell Institute for Medical Research (Camden, NJ). Each SWS-derived fibroblast line was obtained by the referring clinician and grown via a clinical lab service and then sent to us with consent through an approved IRB (Ferreira et al., 2018 (link)). SW1353 was obtained from ATCC.
Fibroblasts and SW1353 were cultured in Dulbecco’s Modified Eagle’s medium (DMEM) containing 1 g/L glucose supplemented with 10% heat-inactivated fetal bovine serum (FBS), 1X Penicillin-Streptomycin (Corning) and 200 mm L-glutamine (Corning). HEK293T cells were cultured in 4.5 g/L glucose DMEM supplemented with the same components as mentioned above.
Xia Z.J., Mahajan S., Paul Daniel E.J., Ng B.G., Saraswat M., Campos A.R., Murad R., He M, & Freeze H.H. (2022). COG4 mutation in Saul-Wilson syndrome selectively affects secretion of proteins involved in chondrogenesis in chondrocyte-like cells. Frontiers in Cell and Developmental Biology, 10, 979096.
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2

Comparison of Fibroblast Cell Lines

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Seven primary human skin fibroblast lines—GM09503 (passage frozen (P), P3), GM01652 (P11), GM01651 (P14), GM00288 (P9), GM01681 (P12), GM01680 (P12), and GM03525 (P7) —were purchased from the Coriell Institute (Camden, NJ, USA). The fibroblasts were cultured in MEM (Gibco) supplemented with 15% (v/v) heat inactivated FBS (Gibco), 1% (v/v) MEM non-essential amino acids (Gibco), and 1% (v/v) Pen-Strep (Gibco) in the incubator (37 °C, 5% CO2). The cells within 7 passages from the initial P were used for RT-qPCR assays.
Lee Y, & Shivashankar G.V. (2020). Analysis of transcriptional modules during human fibroblast ageing. Scientific Reports, 10, 19086.
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3

Generation of hiPSC-Derived Muscle Cells

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De-identified fresh frozen human skeletal muscle tissues were purchased from ProteoGenex Inc. The study protocol was approved by the Institutional Review Board of MD Anderson Cancer Center, University of Texas. Clinical information is summarized in Supplementary Table 1.
All hiPSC studies were approved by HEIP Stem Cell committee of the University of Texas, MD Anderson Cancer Center. De-identified human BMD patient’s donor fibroblasts cells (GM04569, GM05089, GM02298 and GM04981) and healthy donor fibroblast cells (GM09503) were obtained from Coriell Institute and reprogramed to iPS cells at Human Stem Cell Core (Baylor College of Medicine). The human DMD patients donor iPS cells (GM25313) were obtained from Coriell Institute. Clinical information is summarized in Supplementary Table 1. IPS cells were cultured on hESC-Qualified Matrigel (Corning) coated plates and maintained in feeder-free mTeSR™1 medium (Stemcell Technologies). The pluripotency of these iPSCs were verified using Human Pluripotent Stem Cell Functional Identification Kit (R&D Systems, Catalog # SC027). Antibodies with dilution information are listed in Supplementary Table 10. hiPS cells were differentiated to cardiomyocytes and skeletal muscle cells using the STEMdiff™ Cardiomyocyte Differentiation Kit (Stemcell Technologies) and skeletal muscle differentiation protocol previously described54 (link).
Zhang Y., Li Y., Hu Q., Xi Y., Xing Z., Zhang Z., Huang L., Wu J., Liang K., Nguyen T.K., Egranov S.D., Sun C., Zhao Z., Hawke D.H., Li J., Sun D., Kim J.J., Zhang P., Cheng J., Farida A., Hung M.C., Han L., Darabi R., Lin C, & Yang L. (2020). LncRNA H19 Alleviates Muscular Dystrophy Through Stabilizing Dystrophin. Nature cell biology, 22(11), 1332-1345.
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4

Generation of hiPSC-Derived Muscle Cells

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De-identified fresh frozen human skeletal muscle tissues were purchased from ProteoGenex Inc. The study protocol was approved by the Institutional Review Board of MD Anderson Cancer Center, University of Texas. Clinical information is summarized in Supplementary Table 1.
All hiPSC studies were approved by HEIP Stem Cell committee of the University of Texas, MD Anderson Cancer Center. De-identified human BMD patient’s donor fibroblasts cells (GM04569, GM05089, GM02298 and GM04981) and healthy donor fibroblast cells (GM09503) were obtained from Coriell Institute and reprogramed to iPS cells at Human Stem Cell Core (Baylor College of Medicine). The human DMD patients donor iPS cells (GM25313) were obtained from Coriell Institute. Clinical information is summarized in Supplementary Table 1. IPS cells were cultured on hESC-Qualified Matrigel (Corning) coated plates and maintained in feeder-free mTeSR™1 medium (Stemcell Technologies). The pluripotency of these iPSCs were verified using Human Pluripotent Stem Cell Functional Identification Kit (R&D Systems, Catalog # SC027). Antibodies with dilution information are listed in Supplementary Table 10. hiPS cells were differentiated to cardiomyocytes and skeletal muscle cells using the STEMdiff™ Cardiomyocyte Differentiation Kit (Stemcell Technologies) and skeletal muscle differentiation protocol previously described54 (link).
Zhang Y., Li Y., Hu Q., Xi Y., Xing Z., Zhang Z., Huang L., Wu J., Liang K., Nguyen T.K., Egranov S.D., Sun C., Zhao Z., Hawke D.H., Li J., Sun D., Kim J.J., Zhang P., Cheng J., Farida A., Hung M.C., Han L., Darabi R., Lin C, & Yang L. (2020). LncRNA H19 Alleviates Muscular Dystrophy Through Stabilizing Dystrophin. Nature cell biology, 22(11), 1332-1345.
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5

Dermal Fibroblast Culture from Healthy Controls

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Dermal primary fibroblasts derived from healthy controls (GM08429, GM08680, GM03349, GM05565 and GM09503) were obtained from Coriell Institute for Medical Research (Camden, NJ). Each SWS-derived fibroblast line was obtained by the referring clinician and grown via a clinical lab service and then sent to us with consent through an approved IRB.
Fibroblasts were cultured in Dulbecco's Modified Eagle's medium (DMEM) containing 1 g/L glucose supplemented with 10% heat-inactivated fetal bovine serum (FBS) and 1% antibiotic-antimycotic (Life Technologies, Carlsbad, CA, USA).
Xia Z., Zeng X.I., Tambe M.A., Ng B.G., Dong P.D.S., & Freeze H.H. (2021). A single heterozygous mutation in COG4 disrupts zebrafish early development via Wnt signaling.
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