MC3T3-E1 cells were seeded at a density of 2×104 cells/cm2 in prepared 6-well plates covered with gelatin. The standard medium was replaced with osteogenic differentiation medium (Cyagen, USA) when the cells reached 60% confluence and was refreshed every 3 days. After osteogenic induction for 14 and 21 days, samples were fixed with 4% PFA for 30 minutes. Alizarin Red dye solution was added to each well for 5 minutes. After washing with PBS, images were captured using a camera and microscope. The mineral deposits were dissolved in 10 mM cetylpyridinium chloride quantified (Macklin, China), and the optical density (OD) was measured with a Tecan Sunrise instrument at 570 nm.
Sunrise instrument
Manufactured by Tecan
Sourced in China
The Sunrise instrument is a versatile microplate reader designed for a wide range of applications in life science research and clinical diagnostics. It offers high-performance absorbance measurements across a broad wavelength range, enabling reliable and reproducible results for various assays.
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Lab products found in correlation
2 protocols using sunrise instrument
1
Alizarin Red Staining of Osteogenic Differentiation
2
Biofilm Formation Assay for C. difficile
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The biofilm formation assay was performed for the C. difficile R20291 wild-type, lexA mutant, plasmid complemented lexA mutant and strain 630 as described by Dapa and co-workers [28 (link)]. The biofilm was generated in a tissue culture treated 24-well polystyrene plate (CLS3527, Sigma-Aldrich). The optical density OD570 of crystal violet retained by the biofilm was measured on a Sunrise™ instrument (Tecan). Non-inoculated broth medium was used as a negative control. The average OD570 of biofilm cell mass and standard deviation was calculated based on three independent experiments performed in duplicate.
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