Pbs tablets

Manufactured by MP Biomedicals

Sourced in United States

PBS (Phosphate-Buffered Saline) tablets are a ready-to-use solution that, when dissolved in water, creates a buffer with a physiological pH and ionic composition. The tablets are designed to maintain a consistent pH and ionic balance, making them suitable for various laboratory applications.

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Lab products found in correlation

12 protocols using pbs tablets

Comparison of Nicotine Transdermal Delivery Systems

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Phosphate-buffered saline (PBS) of pH 7.4 (consisting of 0.01 M phosphate buffer, 0.0027 M potassium chloride, and 0.137 M sodium chloride) was prepared by dissolving PBS tablets (MP Biomedicals, Solon, OH) in deionized water. Sodium azide (Acros Organics, Morris Plains, NJ) was added to PBS at 0.02% as a bacteriostat.
Two TDS products were investigated in the study. The first TDS was Equate Clear Transdermal System Nicotine Patch, 21 mg/24 h (Nicoderm CQ®) manufactured by Alza Corp (Mountain View, CA), National Drug Code (NDC) of 49035-194-02, lot number 1405762. The second TDS was Aveva Transdermal System Nicotine Patch, 21 mg/24 h manufactured by Aveva Drug Delivery Systems (Miramar, FL), NDC of 0536-5896-88, lot number 42488. Although both systems have the same nominal delivery rate, the two products are of different size and the formulations are also different with respect to drug loading, adhesives, and inactive excipients. The Alza and Aveva nicotine TDS are hereafter referred to as TDS-N1 and TDS-N2, respectively. These two pharmaceutically equivalent nicotine TDS products have different formulations, are not approved as therapeutic equivalents, and are not expected to be bioequivalent to each other.

La Count T.D., Zhang Q., Murawsky M., Hao J., Ghosh P., Dave K., Raney S.G., Talattof A., Kasting G.B, & Li S.K. (2020). Evaluation of Heat Effects on Transdermal Nicotine Delivery In vitro and In Silico Using Heat-Enhanced Transport Model Analysis. The AAPS journal, 22(4), 82.

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Oxcarbazepine Transdermal Delivery Optimization

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OXC was purchased from Euticals Spa, Italy and used as the drug molecule. Cineole (cin), N-methyl-Pyrrolidone (NMP), oleic acid (OA) and Transcutol^® P (TRC) were investigated as penetration enhancers. OA was a gift from Croda Inc., Edison, NJ, USA. TRC and Labrasol (LS) were gifts from Gattefosse Corporation, Paramus, NJ, USA. Cin, NMP, HPLC grade mEthanol and water, Propylene Glycol (PG), Polysorbate 80 (Tween 80), Isopropyl Myristate (IPM), Medium Chain Triglyceride (MCT), Polyethylene Glycol 400 (PEG-400), Sodium Lauryl Sulfate (SLS), Tri Ethanolamine (TEA) and formic acid were obtained from Sigma-Aldrich, Saint Louis, MO, USA. Carbopol 980 was a gift from Lubrizol, Wickliffe, OH, USA. Ethanol was procured from Decon Labs, Inc., King of Prussia, PA, USA. Phosphate-Buffered Saline (PBS) was prepared by dissolving one PBS tablet in 100 mL of water. Add SLS to PBS at 5% and pH was adjusted to 5.8. PBS tablets were purchased from MP Biomedicals, Solon, OH, USA. Dermatomed human cadaver skin from the posterior torso region of 59-year-old male was used for the ex-vivo permeation study and was supplied by New York Firefighter Skin Bank, New York, NY, USA.

Virani A., Puri V., Mohd H, & Michniak-Kohn B. (2023). Effect of Penetration Enhancers on Transdermal Delivery of Oxcarbazepine, an Antiepileptic Drug Using Microemulsions. Pharmaceutics, 15(1), 183.

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Radiolabeled Compound Characterization Protocol

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¹⁴C-Tetraethylammonium bromide, ¹⁴C-salicylic acid,
³H-mannitol, and Ultima Gold scintillation cocktail were
purchased from PerkinElmer Life and Analytical Sciences (Boston, MA).
³H-Estradiol, ¹⁴C-urea, and ³H-Fluconazole
were purchased from Moravek Biomaterials and Radiochemicals (Brea, CA).
³H-Corticosterone was from American Radiolabeled Chemicals (St.
Louis, MO).¹⁴C-indomethacin was from Amersham Life Science (Amersham,
United Kingdom). Chlorhexidine diglucanote, sucrose, and sodium salicylate were
purchased from Sigma–Aldrich (St. Louis, MO). Tetraethylammonium bromide,
mannitol, urea, indomethacin, corticosterone, and sodium azide (NaN₃)
were purchased from Acros Organics (Morris Plains, NJ). Ketorolac tromethamine
and estradiol were purchased from Letco Medical (Decatur, AL). Fluconazole was
from TCI America (Portland, OR). Lidocaine HCl was from Professional Compounding
Center of America (PCCA, Houston, TX). Phosphate buffered saline (PBS), pH 7.4,
consisting of 0.01 M phosphate buffer, 0.0027 M potassium chloride, and 0.137 M
sodium chloride was prepared using PBS tablets (MP Biomedicals, LLC, Solon, OH)
and deionized water (DI water). Triethylamine (HPLC grade), monobasic sodium
phosphate, and glacial acetic acid were purchased from Fisher Scientific (Fair
Lawn, NJ). Acetonitrile (HPLC grade) was from Pharmaco-AAPER (Shelbyville, KY).
All materials were used as received.

, & Leinwand L.A. (2001). Calcineurin inhibition and cardiac hypertrophy: A matter of balance. Proceedings of the National Academy of Sciences of the United States of America, 98(6), 2947-2949.

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Biofunctional Hydrogel for Stem Cell Culture

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Gelatin from porcine skin, Eosin Y, 1-vinyl-2-pyrrolidinone (VP), triethanolamine (TEA), thiazolyl blue tetrazolium bromide (MTT), methacrylic anhydride, trypsin-EDTA, penicillin-streptomycin, 10% sodium dodecyl sulphate (SDS), 1-ethyl-3-(3-dimethylamino) carbodiimide hydrochloride salt (EDC), human mesenchymal stem cells (HMSC), dimethyl sulfoxide (DMSO), DCl and D 2 O were purchased from Sigma-Aldrich. Sodium alginate (G/M:0.6, 190-240 kDa), and PBS tablets were acquired from MP Biomedicals. β-cyclodextrin (β-CD) was purchased from AK scientific. Fetal bovine serum (FBS) was supplied by Hyclone™. Cell culture media (minimum essential media, MEM, without phenol red) was acquired from Gibco™. Ethylenediamine (EDA), N-hydroxysuccinimide (NHS), L-tyrosine and p-toluene sulfonyl chloride were acquired from ACROS Organics.

Jalalvandi E, & Shavandi A. (2019). Shear thinning/self-healing hydrogel based on natural polymers with secondary photocrosslinking for biomedical applications. Journal of the mechanical behavior of biomedical materials, 90.

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Anti-inflammatory Mechanisms of Pharmacological Agents

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PBS tablets and proteinase K were obtained from (MP Bio USA). Formaldehyde, hydrogen peroxide (H2O2), reduced glutathione (GSH), trichloroacetic acid (TCA), 1-chlor-2,4-dinitrobenzene (CDNP), N-(1-Naphthyl) ethylenediamine dihydrochloride, 5,5′-dithio-bis-(2- nitro benzoic acid; DTNB), and caffeic acid phenethyl ester (CAPE) were purchased from (Sigma-Aldrich, USA). Atorvastatin, cephalexin, and mycophenolate were obtained from the local pharmaceutical industry with the highest analytical grade (99% HPLC grade). Mouse monoclonal anti-TNF-α (SC-52B83), mouse monoclonal anti-p-NF-κB (SC-271908), mouse monoclonal anti-COX-2 (SC-514489), mouse monoclonal anti-GFAP (SC-33673), ABC Elite kit (SC-2018), and 3,3′-diaminobenzidine peroxidase (DAB; SC-216567) were purchased from (Santa Cruz Biotechnology, USA). The horseradish peroxidase-conjugated secondary antibody (ab-6789) and mounting media (ab-10431) were obtained from Abcam UK. The p-NF-κB Elisa kit (Cat # SU-B28069) and p-JNK (Cat # SU-B30586) Elisa kit were purchased from (Shanghai Yuchun Biotechnology, China).

Ali A., Shah F.A., Zeb A., Malik I., Alvi A.M., Alkury L.T., Rashid S., Hussain I., Ullah N., Khan A., Koh P.O, & Li S. (2020). NF-κB Inhibitors Attenuate MCAO Induced Neurodegeneration and Oxidative Stress—A Reprofiling Approach. Frontiers in Molecular Neuroscience, 13, 33.

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Isolation and Modification of Erythrocytes

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Experiments in vitro were carried out according to the scheme shown in Figure 1. In the first stage of the experiment, a suspension of erythrocytes was prepared. For this, 150 μl of fresh human blood was centrifuged at 3000 rpm during 5 minutes to separate blood cells from plasma. Plasma was removed, and the volume was brought to that of the original blood sample with PBS, pH 7.4 (PBS Tablets, MP Biomedicals, USA). Hence, in suspension, RBC concentration was the same as in initial blood. The RBCs were washed three times in PBS. In the second stage of the experiment, various modifiers were added to RBCs.

Kozlova E., Chernysh A., Manchenko E., Sergunova V, & Moroz V. (2018). Nonlinear Biomechanical Characteristics of Deep Deformation of Native RBC Membranes in Normal State and under Modifier Action. Scanning, 2018, 1810585.

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Formulation and Characterization of CHX-Loaded Biodegradable Chips

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Phosphate buffered saline (PBS), pH 7.4, consisting of 0.01 M phosphate buffer, 0.0027 M potassium chloride, and 0.137 M sodium chloride, was prepared using PBS tablets (MP Biomedicals, LLC, Solon, OH) and deionized water (DI water). Simulated saliva, pH 8.0, consisting of 0.137 M sodium chloride, 0.0014 M potassium phosphate monobasic, 0.017 M sodium phosphate dibasic (Fisher Scientific, Fair Lawn, NJ) was prepared in DI water. PerioChip^® (CHX gluconate, 2.5 mg) was manufactured by Drexel Pharma Technologies Ltd (Yokneam, Israel) and three different PerioChip^® lots were used. CHX digluconate (20% solution in water), CHX powder, Type A porcine skin gelatin (300 Bloom), Type B bovine skin gelatin (225 Bloom), and glutaraldehyde (GTA) were purchased from Sigma-Aldrich (St. Louis, MO). Pepsin A (powder) was purchased from Acros Organics (Janssen Pharmaceuticalaan, Geel, Belgium). Trimethylamine (HPLC grade), sodium phosphate monobasic, and o-phosphoric acid were purchased from Fisher Scientific (Fair Lawn, NJ). Acetonitrile and methanol (HPLC grade) were from Pharmaco-AAPER (Shelbyville, KY). Release liner (Scotchpak 1022, 3.0 mil) was obtained from 3 M (St. Paul, MN). All materials were used as received.

Murawsky M., Kelm G.R., Kozak D., Qin B., Zou Y, & Li S.K. (2018). Influencing factors on gelatin matrix for chlorhexidine delivery. Drug development and industrial pharmacy, 45(2), 314-322.

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Digitoxigenin Formulation for Bioavailability

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Digitoxigenin and olive oil (highly refined, low acidity) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Alginic acid sodium salt (low viscosity) was from MP Biomedicals (Solon, OH, USA). Tween 85 (Acros Organics, Morris, NJ, USA) and Span 85 (Sigma-Aldrich, St. Louis, MO, USA) were used in the preparation of Digitoxigenin formulations. Other reagents used were isooctane (Acros Organics, Morris, NJ, USA) and calcium chloride dehydrate (Fisher Scientific, Springfield, NJ, USA). Phosphate buffered saline (PBS) of pH 7.4 was prepared by dissolving PBS tablets (MP Biomedicals, Solon, OH, USA) in deionized water, and sodium azide (Acros Organics, Morris, NJ, USA) was added at a concentration of 0.02% (w/v) as a bacteriostat. High performance liquid chromatography (HPLC) grade methanol, water, and formic acid were purchased from Fisher Scientific (Pittsburgh, PA, USA).

Feng X., Wang C., Xu Y., Turley J., Xie Z., Pierre S.V, & Hao J. (2018). Topical Digitoxigenin for Wound Healing: A Feasibility Study. Bioengineering, 5(1), 21.

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Fabrication of PCL-Gelatin-ZnO Scaffolds

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Poly(ε-caprolactone) pellets (PCL, M_w = 80,000), gelatine type B from bovine skin (~225 bloom, M_w = 50,000), 1,1,1,3,3,3-hexafluoro-2-propanol (HFP), and zinc oxide nanoparticles (ZnO, < 100 nm particle size) were all purchased from Sigma-Aldrich (St. Louis, MO, USA) and used as-received. Phosphate-buffered saline solution (PBS, 1%) was prepared by mixing PBS tablets (MP Biomedicals, Santa Ana, CA, USA) and distilled water. The plastic syringes (5 mL) and 27G dispensing needles were obtained from Becton, Dickson and Company (Franklin Lakes, NJ, USA) and CML Supply (Lexington, KY, USA), respectively.

Münchow E.A., Albuquerque M.T., Zero B., Kamocki K., Piva E., Gregory R.L, & Bottino M.C. (2015). Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regeneration. Dental materials : official publication of the Academy of Dental Materials, 31(9), 1038-1051.

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Synthesis and Characterization of Biodegradable Polymers

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l-Lactide 99.5% (Green Square Materials Inc), pentaerythritol tri and tetraacrylate (Photomer 4335), tin(ii) 2-ethylhexanoate 96% (Sn(Oct)₂,Alfa Aesar®), 4-methoxyphenol (SHORA), 2-hydroxy-2-methyl-1-phenylpropanone (I.G.M Omnirad 1173), PBS Tablets (MP Biomedicals), tetrahydrofuran (TEDIA), ethyl acetate (TEDIA), acetone (TEDIA), high-purity grade silica gel, pore size 60 Å, 70–230 mesh (AACASH Exports).

Yu C.F., Rwei S.P, & Shu Y.C. (2023). One-pot synthesis of liquid photocrosslinkable poly(l-lactide) with terminal triacrylate. RSC Advances, 13(4), 2331-2338.

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