Gedatolisib

CNDAC was purchased from biorbyt (via Biozol, Eching, Germany), 5-azacytidine, cytarabine, cladribine, clofarabine, decitabine, and fludarabine from Tocris Biosciences (via Bio-Techne GmbH, Wiesbaden, Germany), 6-thioguanine, ganetespib, molibresib, olaparib, sapacitabine, venetoclax, and vismodegib from MedChemExpress (via Hycultec, Beutelsbach, Germany), daunorubicin, gedatolisib, and volasertib from Selleckchem (Berlin, Germany), gemcitabine from Hexal (Holzkirchen, Germany), GTP and dATP from Thermo Scientific (Dreieich, Germany), and CNDAC-TP from Jena Bioscience GmbH (Jena, Germany).

Gedatolisib (purity: 99.09%) was purchased from Selleck Chemicals (Houston, TX, USA). Paclitaxel (purity: 99.02%), doxorubicin (purity: 99.37%), vincristine (purity: 99.19%), mitoxantrone hydrochloride (purity: 99.9%), topotecan hydrochloride (purity: 100.0%), and cisplatin (purity: 99.62%) were purchased from Energy Chemicals (Shanghai, China). Verapamil hydrochloride (purity: 99.95%) and Ko143 (purity: 99.79%) were purchased from MedChemExpress (NJ, USA). Tritium-labeled paclitaxel and mitoxantrone were obtained from SuYa Medicine (Beijing, China). Fetal bovine serum (FBS), Dulbecco’s modified Eagle’s Medium (DMEM), penicillin/streptomycin, and trypsin were purchased from Corning Incorporated (Corning, NY, USA). G418, DMSO, 3-[4,5-dimethylthiazol-yl]-2,5-diphenyltetrazolium bromide (MTT), propidium iodide (PI), and paraformaldehyde, were purchased from Beyotime Biotechnology (Shanghai, China). Antibody of GAPDH, ABCB1, and ABCG2, Alexa Fluor 488 conjugated goat anti-mouse IgG secondary antibody, HRP-conjugated goat anti-mouse IgG secondary antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). All other chemicals were obtained from Sigma Chemical Co (St. Louis, MO, USA).