The following antibodies were used for immunohistochemical and western blot analyses in the present study: Anti-hypoxia-inducible factor (HIF)-1α antibody (product code ab51608; Abcam), anti-ANX A1 antibody (product code ab88865; Abcam), anti-phosphorylated (p)-44/42 mitogen-activated protein kinase (MAPK) (Thr202/Tyr204) rabbit monoclonal antibody (mAb) (product no. 4370; Cell Signaling Technology, Inc.), anti-p44/42 MAPK rabbit mAb (product no. 4695; Cell Signaling Technology, Inc.), anti-acetyl-α-tubulin rabbit mAb (product no. 5335; Cell Signaling Technology, Inc.), goat anti-rabbit IgG H&L (DyLight® 488; product code ab150077; Abcam), goat anti-mouse (DyLight® 647; product code ab150115; Abcam) and goat anti-rabbit/mouse IRDye-800CW secondary antibodies (product nos. 926-32211 and 926-32210, respectively; LI-COR Biosciences). The immunohistochemical staining kit (cat. no. D01-18) was purchased from OriGene Technologies, Inc.
Goat anti mouse dylight 647
Manufactured by Abcam
Goat anti-mouse (DyLight® 647) is a secondary antibody conjugated with the DyLight® 647 fluorescent dye. It is designed to bind and detect mouse primary antibodies in various immunoassays.
Automatically generated - may contain errors
Lab products found in correlation
Hif 1α antibody, by Abcam (1 mentions)
Goat anti rabbit igg h l dylight 488, by Abcam (1 mentions)
Anti anx a1 antibody, by Abcam (1 mentions)
Ab109367, by Abcam (1 mentions)
Ab19381, by Abcam (1 mentions)
Ab96899, by Abcam (1 mentions)
Goat anti rabbit dylight 488, by Abcam (1 mentions)
Ab150115, by Abcam (1 mentions)
2 protocols using goat anti mouse dylight 647
1
Antibody-based Protein Detection Protocol
2
Immunofluorescence Staining of PRDX2 and β-Catenin
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Coverslips were plated in 6-well plates, and HepG2 cells were added onto them. The following day, cells were fixed with 4% paraformaldehyde, then permeabilized by treating them with PBS containing 0.1% Triton X-100 for 15 min. The slides were washed three times with PBS, then blocked with 2% BSA for 30–45 min at room temperature, The slides were incubated overnight with the following primary antibodies: anti-PRDX2 rabbit monoclonal antibody (1:250, ab109367, Abcam) and anti-β-catenin mouse monoclonal antibody (1:200, ab19381, Abcam) at 4 degrees. The next day, the slides were washed three times with PBST for 5 min each time. Then, the slides were incubated with fluorescent secondary antibody: Goat anti-rabbit-DyLight® 488 (1:1000, ab96899, Abcam) and Goat anti-mouse-DyLight® 647 (1:1000, ab150115, Abcam) for one hour at room temperature in the dark. Afterwards, the slides were washed three times with PBST and observed using a fluorescent microscope.
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