Log In Sign up
The largest database of trusted experimental protocols

Uas ubx

Manufactured by Bloomington Drosophila Stock Center
Visit Supplier
Sourced in United States

The UAS-Ubx is a genetic tool used in Drosophila research. It contains the Ultrabithorax (Ubx) gene under the control of the Upstream Activating Sequence (UAS) promoter. This allows for targeted expression of the Ubx gene in specific tissues or cell types of the Drosophila model organism.

Automatically generated - may contain errors

Lab products found in correlation

Uas mcherry, by Bloomington Drosophila Stock Center (2 mentions) Labialrnai, by Bloomington Drosophila Stock Center (2 mentions) Uas tkvqd, by Bloomington Drosophila Stock Center (2 mentions) Uas dpp, by Bloomington Drosophila Stock Center (2 mentions) Ubxrnai line 1, by Bloomington Drosophila Stock Center (2 mentions) Ubxrnai line 2, by Bloomington Drosophila Stock Center (2 mentions) Dadrnai, by Bloomington Drosophila Stock Center (2 mentions) Uas rpr, by Bloomington Drosophila Stock Center (1 mentions) Uas lifeact gfp, by Bloomington Drosophila Stock Center (1 mentions) Uas mcd8 gfp, by Bloomington Drosophila Stock Center (1 mentions)

3 protocols using uas ubx

1

Genetic Toolkit for Drosophila Research

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fly lines w1118, FRT82, LabialRNAi (BL26753), UAS-mcherry (BL38245), UAS-TkvQD (BL36536), UAS-Dpp (BL1486), UAS-Ubx (BL911), Ubx1 (BL529), UbxRNAi line 1 (BL31913), UbxRNAi line 2 (BL34993), DadRNAi (BL33759) were obtained from Bloomington Drosophila Stock Center. esg-Gal4, UAS-GFP was a gift from Shigeo Hayashi; FRT82B, Dad212 from Hannele Ruohola-Baker; Btl-Gal4ts, UAS-GFP from Dirk Bohmann; Dad::nlsGFP from Georgios Pyrowolakis; Su(H)-GBE-lacZ from Sarah Bray; esgtsF/O (esgGal4, tubG80ts, UAS-GFP; UAS-flp, act > STOP > Gal4) from Huaqi Jiang; UAS::Dad from Thomas Kornberg; NP1::Gal4 from Dominique Ferrandon; MARCM82 (hsFlp; tub-Gal4, UAS-GFP; FRT82, tubGal80) from Norbert Perrimon.
Flies were cultured on yeast/molasses-based standard fly food (Recipe: 10 L H2O, 138 g agar, 220 g molasses, 750 g malt extract, 180 dry yeast, 800 g corn flour, 100 g soy flour, 62.5 ml propionic acid, 20 g Methyl 4-Hydroxybenzoate, and 72 ml ethanol) at 25 °C with a 12 h light/dark cycle. For TARGET (tubGal80ts) experiments, flies were raised at 18 °C to allow Gal80 to inhibit Gal4, and 3–4 days after eclosion shifted to 29 °C to inhibit Gal80 and to allow Gal4 to drive UAS-linked transgene expression.
Li H., Qi Y, & Jasper H. (2016). Ubx dynamically regulates Dpp signaling by repressing Dad expression during copper cell regeneration in the adult Drosophila midgut. Developmental biology, 419(2), 373-381.
+ Open protocol
+ Expand
2

Targeted Gene Expression in Drosophila

Check if the same lab product or an alternative is used in the 5 most similar protocols
Drosophila stocks were maintained at 25°C. The w1118 strain was used as wild type. The targeted expression experiments were performed using the UAS-GAL4 system (Brand and Perrimon, 1993 (link)) on the following GAL4 and UAS lines: eme-GAL4 (kindly provided by R. Bodmer, Burnham Institute, San Diego, CA, USA); UAS-Rpr (BL5824), UAS-Antp (BL7301), UAS-Ubx (BL911), UAS-AbdA (BL912), UAS-AbdB (BL913), UAS-mcd8-GFP (BL32184) and UAS-LifeAct-GFP (BL58718) from Bloomington Stock Center; UAS-Tup RNAi (45859) and UAS-Eve RNAi (9284) from VDRC; UAS-Tin (kindly provided by M. Frasch, Erlangen-Nürnberg University, Germany); UAS-Lbe (Jagla et al., 1997 (link)); and UAS-Kr (a gift from G. Vorbrüggen, Max Planck Institute, Goettingen, Germany). The following additional lines were used: UAS-Antp RNAi (101774) and UAS-AbdA RNAi (106155) from VDRC; Antp25 (3020) from Bloomington Stock Center; and AbdAM1 (kindly provided by L. Perrin; Aix-Marseille University, France). The Tup-GFP line was kindly provided by Stephan Thor (Linköping University, Sweden), the Hand-GFP line was a gift from E. Olson (Utah University, Salt Lake City, USA) and the UAS-H2B::YFP line was kindly provided by Michel Gho (IBPS, Paris, France). UAS-Ubx was re-balanced with TM3, twi-lacZ, and homozygous mutant embryos were selected by absence of β-galactosidase staining.
Zmojdzian M., de Joussineau S., Da Ponte J.P, & Jagla K. (2018). Distinct subsets of Eve-positive pericardial cells stabilise cardiac outflow and contribute to Hox gene-triggered heart morphogenesis in Drosophila. Development (Cambridge, England), 145(2), dev158717.
+ Open protocol
+ Expand
3

Genetic Toolkit for Drosophila Research

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fly lines w1118, FRT82, LabialRNAi (BL26753), UAS-mcherry (BL38245), UAS-TkvQD (BL36536), UAS-Dpp (BL1486), UAS-Ubx (BL911), Ubx1 (BL529), UbxRNAi line 1 (BL31913), UbxRNAi line 2 (BL34993), DadRNAi (BL33759) were obtained from Bloomington Drosophila Stock Center. esg-Gal4, UAS-GFP was a gift from Shigeo Hayashi; FRT82B, Dad212 from Hannele Ruohola-Baker; Btl-Gal4ts, UAS-GFP from Dirk Bohmann; Dad::nlsGFP from Georgios Pyrowolakis; Su(H)-GBE-lacZ from Sarah Bray; esgtsF/O (esgGal4, tubG80ts, UAS-GFP; UAS-flp, act > STOP > Gal4) from Huaqi Jiang; UAS::Dad from Thomas Kornberg; NP1::Gal4 from Dominique Ferrandon; MARCM82 (hsFlp; tub-Gal4, UAS-GFP; FRT82, tubGal80) from Norbert Perrimon.
Flies were cultured on yeast/molasses-based standard fly food (Recipe: 10 L H2O, 138 g agar, 220 g molasses, 750 g malt extract, 180 dry yeast, 800 g corn flour, 100 g soy flour, 62.5 ml propionic acid, 20 g Methyl 4-Hydroxybenzoate, and 72 ml ethanol) at 25 °C with a 12 h light/dark cycle. For TARGET (tubGal80ts) experiments, flies were raised at 18 °C to allow Gal80 to inhibit Gal4, and 3–4 days after eclosion shifted to 29 °C to inhibit Gal80 and to allow Gal4 to drive UAS-linked transgene expression.
Li H., Qi Y, & Jasper H. (2016). Ubx dynamically regulates Dpp signaling by repressing Dad expression during copper cell regeneration in the adult Drosophila midgut. Developmental biology, 419(2), 373-381.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!