Log In Sign up
The largest database of trusted experimental protocols

Cell viability kit

Manufactured by Promega
Visit Supplier

The Cell Viability Kit is a laboratory instrument designed to measure the viability and proliferation of cells. It uses a colorimetric or fluorometric assay to quantify the number of living cells in a sample.

Automatically generated - may contain errors

Lab products found in correlation

P24 elisa kit, by PerkinElmer (2 mentions) Calcusun, by Biosoft (2 mentions) Methyl thiazolyl tetrazolium (mtt), by Promega (1 mentions) Microplate reader, by Promega (1 mentions) Luciferase assay kit, by Biotium (1 mentions) Prism 7, by GraphPad (1 mentions) Facsdiva software, by BD (1 mentions) Facscanto, by BD (1 mentions)

Close spelling variants of this product

MTS cell viability kit CellTiter-Glo 2.0 Cell Viability kit Cell Titer-Glo Luminescent Viability Assay kit ATP content cell viability assay kit CellTiter-Glo Luminescent Cell Viability Assay kit Cell Titer-Blue H Cell Viability assay kit CellTiter-Fluor™ Cell Viability Assay kit CellTiter-Blue Cell Viability Assay kit CellTitre-Glo® Luminescent Cell Viability Assay kit CellTiter-Glo Luminescent Cell Viability kit

5 protocols using cell viability kit

1

Antiviral Screening of Test Compounds Against HIV-1

Check if the same lab product or an alternative is used in the 5 most similar protocols
HIV-1 NL4–3 (multiplicity of infection = 0.001) was used to infect MT4 cells in the presence of various concentrations of the test compounds, and the CellTiter-Glo reagent was added in parallel for cytotoxicity studies.5 Virus replication was analyzed on day-4 postinfection using a p24 ELISA kit from PerkinElmer, and cytotoxicity was determined using a cell viability kit provided by Promega. The compound concentration that inhibited HIV-1 replication by 50% (EC50) and decreased the cell viability by 50% (IC50) was calculated by using the biostatistic software Calcusun (Biosoft). Gnidimacrin was used as a positive control.
Otsuki K., Li W., Miura K., Asada Y., Huang L., Chen C.H., Lee K.H, & Koike K. (2020). Isolation, Structural Elucidation, and Anti-HIV Activity of Daphnane Diterpenoids from Daphne odora. Journal of natural products, 83(11), 3270-3277.
+ Open protocol
+ Expand
2

Antiviral Potency and Cytotoxicity of Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
HIV-1 NL4–3 (multiplicity of infection = 0.001) was used to infect MT4 cells in the presence of different concentrations of the test compounds, and cytotoxicity of these compounds to MT4 cells was evaluated by using a cell viability kit provided by Promega.8 (link) Fresh medium containing appropriate concentrations of the compounds was added to the culture 48 h after infection to maintain normal cell growth. Virus replication was analyzed on day-4 postinfection using a p24 ELISA kit from PerkinElmer. The CellTiter-Glo Luminescent Cell Viability Assay is a simple method of determining the viability of the cells in culture based on quantitation of ATP in metabolically active cells. The CellTiter-Glo reagent was added to the MT4 cells that were cultured parallel to the antiviral assays. The biostatistic software Calcusun (Biosoft) was used to calculate the compound concentration that inhibited HIV-1 replication by 50% (EC50) and decreased the cell viability by 50% (IC50). Gnidimacrin was used as a positive control.
Otsuki K., Zhang M., Yamamoto A., Tsuji M., Tejima M., Bai Z.S., Zhou D., Huang L., Chen C.H., Lee K.H., Li N., Li W, & Koike K. (2020). Anti-HIV Tigliane Diterpenoids from Wikstroemia scytophylla. Journal of natural products, 83(12), 3584-3590.
+ Open protocol
+ Expand
3

Cytotoxicity Assay for MT4 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cytotoxicity of the purified compounds toward MT4 cells was determined by using a cell viability kit provided by Promega. The CellTiter-Glo luminescent cell viability assay is a simple method of determining the viability of the cells in culture based on quantitation of ATP in metabolically active cells. The CellTiter-Glo reagent was added to MT4 cells that were cultured parallel to the antiviral assays. The cytotoxic concentration that caused the reduction of viable cells by 50% (CC50) was calculated from the dose–response curve using CalcuSyn.
Yan M., Lu Y., Chen C.H., Zhao Y., Lee K.H, & Chen D.F. (2015). Stelleralides D–J and Anti-HIV Daphnane Diterpenes from Stellera chamaejasme. Journal of natural products, 78(11), 2712-2718.
+ Open protocol
+ Expand
4

Vero Cell Cytotoxicity Assay for TB Compounds

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Vero cells (ATCC CCL-81) were used to test cytotoxicity as described previously (18 (link)). Briefly, Vero cells were plated at a concentration of 105 cells/well in 96-well plates and incubated for 2 to 3 h to allow the cells to settle. Test compounds were serially diluted separately in Eagle’s minimal essential medium to generate test concentrations ranging from 200 to 0.1× M. tuberculosis MIC. The serial dilutions were then added to the plated cells and incubated for 48 h at 37°C. The viability of Vero cells exposed to each compound was determined using an MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide] cell viability kit (Promega).
Sukheja P., Kumar P., Mittal N., Li S.G., Singleton E., Russo R., Perryman A.L., Shrestha R., Awasthi D., Husain S., Soteropoulos P., Brukh R., Connell N., Freundlich J.S, & Alland D. (2017). A Novel Small-Molecule Inhibitor of the Mycobacterium tuberculosis Demethylmenaquinone Methyltransferase MenG Is Bactericidal to Both Growing and Nutritionally Deprived Persister Cells. mBio, 8(1), e02022-16.
+ Open protocol
+ Expand
5

Luciferase-Based Cell Proliferation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells expressing luciferase were seeded in a 96-well plate at 100 cells per well in triplicate. Cell proliferation was determined through luciferase activity every 24 hours for 6 consecutive days with a luciferase assay kit (Biotium) or cell viability kit (Promega) and a microplate reader (Turner BioSystems). Relative luciferase units were normalized with background subtraction. Nonlinear regression curve fit was performed using exponential growth equation, and two-way ANOVA was used for the analysis of statistical significance (GraphPad Prism 7.0). Cell-cycle profiling was performed in quadruplicate by labeling the cells with 4′,6-diamidino-2-phenylindole (DAPI) to a final concentration of 10 μg/mL. Cells were then analyzed by flow cytometry (BD FACSCanto, BD Biosciences) with BD FACSDiva Software (BD Biosciences).
Tiburcio P.D., Xiao B., Chai Y., Asper S., Tripp S.R., Gillespie D.L., Jensen R.L, & Huang L.E. (2018). IDH1R132H is intrinsically tumor-suppressive but functionally attenuated by the glutamate-rich cerebral environment. Oncotarget, 9(80), 35100-35113.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!