The iHPCs were dissociated into single cells with TrypLE™ Express and counted, and 5 × 104 cells were suspended in 100 μL DPBS with 5% BSA. Then the cells were incubated with BV421-conjugated anti-human CD34, FITC-conjugated CD38, BV510-conjugated CD43, and APC-conjugated CD41a (BD Biosciences, Franklin Lakes, NJ, USA) at 4 °C for 30 min in the dark. The stained cells were washed twice using DPBS and characterized by flow cytometry. The iMKs were incubated with BV421-conjugated anti-human CD34, BV510-conjugated CD43, and APC-conjugated CD41a (BD Biosciences, Franklin Lakes, NJ, USA) at 4 °C for 30 min in the dark and analyzed by flow cytometry.
Zhao J., Zhou M., Wang Z., Wu L., Hu Z, & Liang D. (2022). Ectopic Expression of FVIII in HPCs and MSCs Derived from hiPSCs with Site-Specific Integration of ITGA2B Promoter-Driven BDDF8 Gene in Hemophilia A. International Journal of Molecular Sciences, 23(2), 623.
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