Blood samples were drawn after an overnight fast (12 hours). Blood pressure was measured by an automatic upper arm monitor (Omron M6; OMRON Healthcare Europe, Hoofddorp, the Netherlands). Blood lipids, glucose, insulin, and C‐reactive protein were measured by standard laboratory methods at Uppsala University Hospital. Homeostasis model assessment of insulin resistance was calculated as glucose×insulin/22.5. ELISA was used to determine proinsulin (Mercodia, Uppsala, Sweden), PCSK9 (CycLex, Nagano, Japan), FGF21, IL‐6, IL‐8, IL‐15, IL‐1 receptor antagonist, TNFα, vascular cell adhesion molecule‐1, intercellular adhesion molecule‐1, E‐selectin, endostatin, and TNF receptor 2 (R&D Systems, Minneapolis, MN). Serum unesterified lathosterol was determined by isotope dilution mass spectrometry and corrected for total cholesterol.7 (link) Total NEFA were analyzed using the NEFA FS response kit (Cat. No. 157819910921; DiaSys Diagnostic Systems, Holzheim, Germany), an enzymatic end point method automatically measured on Response 910 (DiaSys Diagnostic Systems) according to the manufacturer's instructions. Von Willebrand factor was measured by ELISA with antisera from Dako (Glostrup, Denmark). The assay was calibrated against Liatest (DiagnosticaStago, Asnieres, France).
Liatest
Manufactured by Diagnostica Stago
Sourced in France
Liatest is a laboratory coagulation testing instrument manufactured by Diagnostica Stago. It is designed to perform a variety of coagulation tests, including prothrombin time (PT), activated partial thromboplastin time (aPTT), and other specialized coagulation assays. The Liatest provides automated, reliable, and efficient coagulation testing to support clinical decision-making.
Automatically generated - may contain errors
Lab products found in correlation
E selectin, by R&D Systems (1 mentions)
Il 1 receptor antagonist, by R&D Systems (1 mentions)
Vascular cell adhesion molecule 1, by R&D Systems (1 mentions)
Interleukin 6 (il 6), by R&D Systems (1 mentions)
Proinsulin, by Mercodia (1 mentions)
Tnf α, by R&D Systems (1 mentions)
Endostatin, by R&D Systems (1 mentions)
Il 15, by R&D Systems (1 mentions)
Sta fibrinogen, by Diagnostica Stago (1 mentions)
Sta ptt a, by Diagnostica Stago (1 mentions)
Sta staclot, by Diagnostica Stago (1 mentions)
Asserachrom protein c, by Diagnostica Stago (1 mentions)
3 protocols using liatest
1
Metabolic and Inflammatory Biomarkers Assessment
2
Coagulation Factors and Inhibitor Assays
3
Quantitative Determination of Hemostatic Factors
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Quantitative determination of protein C antigen (%) was performed using a commercially available enzyme immunoassay Asserachrom Protein C (Diagnostica Stago, Asnieres, France). Protein C activity (%) was measured with a Clotting Assay STA-Staclot (Diagnostica Stago). Total protein S (%) was measured with a microlatex particle-mediated immunoassay Liatest (Diagnostica Stago). An enzyme-linked immunosorbent assay Imulise Monoclonal (Diagnostica Stago) was used to determine free protein S (%). A clotting assay (Bioclot; Diagnostica Stago) was used to study the activity of protein S (%). Quantitative determination of sEPCR (ng/mL) was performed by an enzyme immunoassay (Asserachrom; Diagnostica Stago, Asnieres, France). Quantitation of sTM (ng/mL) was determined by an enzymelinked immunosorbent assay (Imubind; American Diagnostica Inc, Stamford, Connecticut). The coefficients of variation (both interassay and intra-assay reproducibility) were within 6.1% except for protein S activity where they were less than 11.8%.
All laboratory procedures were carried out in full compliance with the tests used. The indicators were determined twice in each sample, taking the average value. Refreezing of samples was not allowed.
All laboratory procedures were carried out in full compliance with the tests used. The indicators were determined twice in each sample, taking the average value. Refreezing of samples was not allowed.
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