To examine
the surface structure of experimental patches, a S-3400N scanning
electron microscope (Hitachi, Chiyoda, Japan) was used. The surface
of xenopericardial patches was used as a positive control. Samples
were mounted using conductive tape and a gold/palladium (Au/Pd) coating
of 15 nm thickness was then sputtered on using the Leica EM ACE200
Vacuum Coater (Leica Microsystems, Austria). Imaging was performed
at 5 kV under high vacuum conditions.
To evaluate platelet aggregation
upon the contact with PRP, vascular patches (
n =
3 samples per group, 0.5 cm
2) were incubated in 300 μL
PRP at 37 °C for 2 h. Samples were then washed with PBS (pH—7.4)
to remove the unabsorbed plasma. Samples were fixed in a 2% glutaraldehyde
solution, thoroughly washed with PBS, dehydrated in ascending concentrations
of ethanol (30–100%, 15 min each), and finally dried at room
temperature. Samples were mounted, sputtered, and imaged as described
above. Nine representative fields of view were randomly selected and
platelet adhesion was evaluated using the PDI
44 (link) where,
NI is the
number of type I platelets;
NII is the
number of type II platelets;
NIII is the
number of type III platelets;
NIV is the
number of type IV platelets;
NV is the
number of type V platelets; and
Ntotal is the total platelet count (
Figure 9).
Sevostianova V.V., Antonova L.V., Mironov A.V., Yuzhalin A.E., Silnikov V.N., Glushkova T.V., Godovikova T.S., Krivkina E.O., Bolbasov E., Akentyeva T.N., Khanova M.Y., Matveeva V.G., Velikanova E.A., Tarasov R.S, & Barbarash L.S. (2020). Biodegradable Patches for Arterial Reconstruction Modified with RGD Peptides: Results of an Experimental Study. ACS Omega, 5(34), 21700-21711.
