Mascot software version

Manufactured by Matrix Science

Sourced in United States

MASCOT software is a bioinformatics tool for the identification and characterization of proteins from mass spectrometry data. It provides a platform for the analysis and interpretation of mass spectrometry-based proteomic experiments.

Automatically generated - may contain errors

Lab products found in correlation

Ltq orbitrap xl, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Mascot (941 citations) Mascot software (278 citations) Mascot software version 2.3.02 (30 citations) Mascot version (7 citations) MASCOT software package version 2.2.06 (3 citations) Mascot database search software version 2.2 (2 citations)

2 protocols using mascot software version

Mascot Database Search Protocol for Proteomics

Check if the same lab product or an alternative is used in the 5 most similar protocols

MS/MS Ion Search: raw MS/MS spectra were submitted in Mascot Generic Format and searched against SwissProt and NCBInr databases using a licensed MASCOT software version 2.2.04 (matrix science, Boston, MA, USA). Taxonomy was limited to Homo sapiens and a maximum of one missed cleavage was permitted for tryptic digestion. Carbamidomethylation of cysteine residues and oxidation of methionine residues were set as fixed and variable modifications, respectively. MALDI-TOF-TOF was chosen as instrument; consequently, the peptide charge was set to “1+”. The peptide tolerance ranged from 25 ppm to 15 ppm/0.2 Da and fragment tolerance ranged from 0.3 Da to 0.1 Da.

Wex B., Safi R.M., Antonios G., Zgheib P.Z., Awad D.B., Kobeissy F.H., Mahfouz R.A., El-Sabban M.M, & Yazbek S.N. (2018). SLC35B4, an Inhibitor of Gluconeogenesis, Responds to Glucose Stimulation and Downregulates Hsp60 among Other Proteins in HepG2 Liver Cell Lines. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(6), 1350.

+ Open protocol

+ Expand

Brain Tissue Proteome Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Three SE-HPLC fractions from brain tissue extract were analyzed in an LTQ Orbitrap XL (Thermo Scientifi c). Samples volume was reduced from 400 to 50 μ L using a SpeedVac concentrator (Savant SC250EXP, Thermo Scientifi c) and then processed as described previously [7] . Briefl y, proteins were denatured, reduced, alkylated and trypsin digested. Peptides were analyzed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), and the resulting spectra were searched separately against the Human UniprotKB-SwissProt Database 2013_10 (containing 88266 forward protein sequences) by Mascot soft ware (version 2.2, Matrix Science).

Martínez-Morillo E., Childs C., García B.P., Álvarez Menéndez F.V., Romaschin A.D., Cervellin G., Lippi G, & Diamandis E.P. (2015). Neurofilament medium polypeptide (NFM) protein concentration is increased in CSF and serum samples from patients with brain injury. Clinical chemistry and laboratory medicine, 53(10).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!