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2 protocols using methyl palmitoleate

1

Comprehensive Virgin Olive Oil Analysis

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The chemical reagents used for VOO characterization were of GC or HPLC grade. Methanol, acetonitrile, o-phosphoric acid and the Folin-Ciocateu (F-C) reagent were provided from Sigma-Aldrich (Steinheim, Germany), n-hexane from Hipersolv Chromanorm (Pennsylvania, USA), Na2CO3 anhydrous from Acros organics (New Jersey, USA) and KOH from Fisher Scientific (Loughborough, UK). Regarding the commercial standards, methyl palmitate, methyl palmitoleate, methyl stearate, methyl oleate, methyl linoleate, methyl linolenate, hydroxytyrosol, tyrosol, p-coumaric acid, luteolin, and apigenin were purchased from Sigma-Aldrich (Steinheim, Germany) and Fluka (Steinheim, Germany), vanillic and syringic acid standards were obtained from Acros organics (New Jersey, USA), gallic acid from Fisher Scientific (Loughborough, UK), and oleacein and oleocanthal were supplied by Prof. Prokopios Magiatis (Athens, Greece). Finally, deionized water (18 MΩ cm) from a Milli-Q water purification system (Millipore, Bedford, MA, USA) was used to prepare the mobile phases for HPLC analysis.
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2

Comprehensive Collection of Lipid Standards

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Balsam of Peru (W211613), balsam of Peru oil (W211710), benzyl cinnamate (234214), benzyl benzoate (B9550), geranylgeraniol (G3278), farnesol (277541), geranylgeranyl acetone (G5048), geraniol (163333), squalene (S3626), geranyl acetone (250716), vitamin K1 (V3501), vitamin K2 (V9378), vitamin A (R7632), vitamin E (T3251), vitamin D3 (C9756), coenzyme Q2 (C8081), coenzyme Q0 (D9150), coenzyme Q4 (C2470), coenzyme Q6 (C9504), coenzyme Q10 (C9538), palmitoleic acid (P9417), methyl palmitoleate (P9667), cis-11-hexadecenal (249084), palmityl acetate (P0260), palmitoleyl alcohol (P1547), lauryl palmitoleate (P1642), oleamide (O2136), palmitoyl ethanolamide (P0359), tetradecanoic acid ethylamide (R425567), N-oleoyl glycin (O9762), N,N-dimethyl tetradecanamide (S347388), and 1-dodecyl-2-pyrrolidinone (335673) were obtained from Sigma-Aldrich (St. Louis, MO). Coenzyme Q1 (270–294-M002) was obtained from Alexis Biochemicals.
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