The use and care of the athymic nude mice were approved by the Research Ethics and Regulations Committee of Chongqing Medical University, Chongqing, China. The animals were obtained from and housed in the Experimental Animal Research Center of Chongqing Medical University. The iKera-FLuc cells were stably transduced with the firefly luciferase-expressing retroviral vector pSEB-FLuc as described [31 (link),52 (link),53 (link)]. Exponentially growing iKera-FLuc cells were resuspended and injected subcutaneously into the flanks of athymic nude mice (6-week old, male, 2 × 106 cells per injection, and 4 sites per mouse). Potential subcutaneous mass growth was assessed at 3, 7 and 14 days after implantation by whole body bioluminescence imaging using the IVIS Lumina Series III In Vivo Imaging System (PerkinElmer, Waltham, MA). The acquired data were quantitatively analyzed by the Living Image Software (PerkinElmer).
Ivis lumina series 3 in vivo imaging system
Manufactured by PerkinElmer
Sourced in United States
The IVIS Lumina Series III In Vivo Imaging System is a laboratory equipment designed for non-invasive, real-time imaging of living small animal models. It utilizes bioluminescence and fluorescence detection technologies to visualize and quantify biological processes within the subject.
Automatically generated - may contain errors
Lab products found in correlation
Living image software, by PerkinElmer (3 mentions)
C3h hej mice, by Jackson ImmunoResearch (1 mentions)
Visualsonics, by Fujifilm (1 mentions)
Living image in vivo imaging software, by PerkinElmer (1 mentions)
Matrigel, by Corning (1 mentions)
Matrigel, by BD (1 mentions)
Living image 4 3 1 service pack 2, by PerkinElmer (1 mentions)
9 protocols using ivis lumina series 3 in vivo imaging system
1
Xenograft Tumor Modeling in Athymic Mice
2
In Vivo Luminescence Imaging of HNSCC
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Luminescence imaging was performed in vivo in an orthotopic murine model of head and neck cancer. AT-84 cells were a gift from Dr. Michael Story (University of Texas Southwestern Medical Center). AT-84 cells (2 × 106 cells in 50 µL of sterile PBS) were orthotopically implanted in male C3H/HeJ mice (Jackson Laboratories, Bar Harbor, ME) in the head and neck region according to an adaptation of a previously described procedure [19 (link)]. At 22 d after implantation, the tumors reached an approximate size of 5–8 mm. Mice were then intravenously injected with 30 mg/kg of free ML18J03 (1% DMSO in PBS) or Mic-ML18J03 (in PBS) through the tail vein. The tumor region was then longitudinally imaged using the IVIS Lumina Series III in vivo imaging system (λExc = 440 nm; λEmi = 710 nm; PerkinElmer, Waltham, MA, USA) at 1, 3, 6, 19, 24, and 48 h after administration. Autofluorescence signals for each animal prior to administration of free ML18J03 or Mic-ML18J03 were subtracted, and the corrected in vivo luminescence signals have been used throughout the study.
3
In Vivo Tumor Imaging with ICG
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After the tumor oxygenation measurements, mice (under anesthesia) were scanned for ICG’s fluorescence using IVIS® Lumina Series III In Vivo imaging system (Perkin Elmer Inc) at different time intervals using 780 nm excitation filter and 840 nm emission filter. After the whole-body imaging, at 24 h post tail vein injection of nanodroplets, mice were euthanized, the tumor and other major organs (kidneys, liver, spleen, lungs, and heart) were harvested and imaged with the IVIS fluorescence imaging system to quantify ICG fluorescence. Autofluorescence and background were subtracted from each organ with data from an untreated mouse. Quantification of the fluorescence signal from the region of interest was performed using Living Image software (PerkinElmer).
4
Fluorescence and Photoacoustic Imaging Protocol
5
Bioluminescence Imaging of Tumor Growth
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Tumor growth was monitored by bioluminescence imaging using an IVIS Lumina Series III In vivo imaging system (PerkinElmer). Anesthetized animals were administered D-luciferin (100 mg/kg of body weight), intraperitoneally and bioluminescence was recorded five minutes past administration. Bioluminescence signal intensity was reported as radiance- a measurement of photons emitted from the subject, in the units of photons/s/cm2/sr. The radiance values were log-normalized and tabulated.
6
Ovarian Cancer Cell Xenograft Imaging
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shScr- or shSnail-expressing OVCAR8-ffluc cells were injected into the ovarian bursa of nude mice at 1:1 with Matrigel (354248; Corning, Corning, NY, USA) at 2.5 × 105 cells per mouse (shScr group: n = 5, shSnail group: n = 4). After intraperitoneal injection of luciferin, the mice were imaged with an IVIS Lumina Series III In Vivo imaging system (PerkinElmer, Waltham, MA, USA). Live imaging was performed weekly and the bioluminescent images were analysed using Living Image In Vivo Imaging Software (PerkinElmer, Waltham, MA, USA) to assess tumour burden at primary and metastatic sites.
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Tumor Imaging Using IVIS Lumina
8
Establishment and Analysis of A549-Luc-C8 Xenografts
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Six-week-old male BALB/c nude mice were used to establish xenografts. For each injection, 1 × 106 A549-Luc-C8 cells were collected and resuspended in 100 μl of ice-cold 20% Matrigel (BD Biosciences, San Jose, CA, USA) in PBS. This 100 μl solution was injected subcutaneously into the thighs of these nude mice using a 22-gauge needle. The transplanted A549-Luc-C8 cells were allowed to grow for 2 weeks, following which the xenografts with A549-Luc-C8 cells were divided into two groups according to the tumor volume, each group with 13 male nude mice. The two groups of animals received intratumoral injections of PBS or LIP (20 μg/kg) into the tumor sites every 2 days. Mice were monitored using the IVIS Lumina Series III In Vivo Imaging System (PerkinElmer, Waltham, MA, USA) twice weekly. Tumor length and width were measured using calipers, following which the tumor volumes were calculated using the equation (L × W2)/2, where L is the length of the tumor and W is the width of the tumor. Human tumor xenografts in the nude mouse model were allowed to grow for a month after injection. At the end of the experiment, the animals (n = 13) were sacrificed, their tumors were separated from the surrounding muscles, weighed, and analyzed using Prism 7 software.
9
In Vivo Biodistribution of Fluorescent-Labeled Nanoparticles
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All animal experiments were performed in compliance with the UK Animals (Scientific Procedures) Act 1986 and UK Home Office Code of Practice for the Housing and Care of Animals Used in Scientific Procedures (Home Office 1989). In vivo experimentation was adhered to the project licence approved by the King's College London animal welfare and ethical review body (AWERB) and UK Home Office. Female Balb/c mice (∼20 g) aged 4-6 weeks (Envigo) were inoculated subcutaneously with CT-26 cells (1 × 10 6 cells in 0.1 mL PBS) at both lower flanks. DiR was incorporated in the formulation at 2.5 wt% of m-PEG-PLGA copolymer and DiR-labeled P-NP (P-NP-DiR) was prepared as described previously. 10 Mice (n = 3) were intravenously injected with P-NP-DiR in PBS solution and scanned at 1, 4, and 24 h post injection using an IVIS Lumina Series III In Vivo Imaging System (PerkinElmer, UK). Untreated animals were included as controls. Animals were anesthetized with 1.5% isoflurane/98.5% oxygen to maintain sedation during the imaging procedure. Ex vivo imaging was carried out for excised major organs (heart, lung, liver, spleen, and kidney) including tumors. Fluorescence images were obtained using DiR filter (740/790 nm for excitation/emission wavelengths) and analysed using Living Image® 4.3.1 Service Pack 2 software (PerkinElmer, UK).
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