Anti β actin sc 130656

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-β-actin (sc-130656) is a primary antibody that recognizes the β-actin protein. β-actin is a ubiquitously expressed cytoskeletal protein that is involved in various cellular processes. This antibody can be used to detect and quantify the expression levels of β-actin in biological samples.

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Lab products found in correlation

Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Anti p iκb α, by Santa Cruz Biotechnology (1 mentions) Anti nfκb p65 sc 372, by Santa Cruz Biotechnology (1 mentions) Anti iκbα sc 371, by Santa Cruz Biotechnology (1 mentions) Anti rabbit or anti mouse horseradish peroxidase hrp conjugated antibody, by Santa Cruz Biotechnology (1 mentions) Caspase glo 3 7 kit, by Promega (1 mentions) Anti p53 9282, by Cell Signaling Technology (1 mentions) Sulforhodamine b, by Merck Group (1 mentions) Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions) Propidium iodide, by Merck Group (1 mentions) Paclitaxel, by Merck Group (1 mentions) 2 7 dichlorofluorescein diacetate dcfh da, by Merck Group (1 mentions)

2 protocols using anti β actin sc 130656

Anti-inflammatory Effects of Dexmedetomidine

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Dexmedetomidine was purchased from Heng Rui Medicine Co., Ltd. (Jiangsu, China). The following primary antibodies (Santa Cruz Biotechnology Inc., CA, USA) were used for Western blot: anti-β-actin (sc-130656), anti-iNOS (sc-649), anti-COX-2 (sc-23983), anti-NF-κBp65 (sc-372), anti-IκBα (sc-371), and anti-p-IκBα (sc-101713). TNF-α (H052), IL-1β (H002), and IL-6 (H007) ELISA kits were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Primers were synthetized by Kangcheng Biotechnology (Guangzhou, China).

Wang L., Liu H., Zhang L., Wang G., Zhang M, & Yu Y. (2016). Neuroprotection of Dexmedetomidine against Cerebral Ischemia-Reperfusion Injury in Rats: Involved in Inhibition of NF-κB and Inflammation Response. Biomolecules & Therapeutics, 25(4), 383-389.

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Anti-cancer Activity Assays Protocol

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Paclitaxel, dimethylsulfoxide (DMSO), cycloheximide, trichloroacetic acid, sulforhodamine B (SRB), propidium iodide (PI), and 2´,7´-dichlorofluorescein-diacetate (DCFH-DA) were purchased from Sigma-Aldrich. The mitochondrial transmembrane potential (MTP) assay kit was purchased from Cayman Chemical Company. RNAse was purchased from Invitrogen. Caspase-Glo 3/7® kit was purchased from Promega corporation. Roswell Park Memorial Institute Media (RPMI-1640), fetal bovine serum (FBS) and antibiotic-antimycotic were purchased from Gibco. PhosphoSafe™ was purchased from Novagen. Pierce™ BCA Protein assay kit was obtained from Thermo Fisher Scientific. Primary antibodies: anti-procaspase-3 (9662), anti-procaspase-7 (9492), anti-Bcl-2 (15071), anti-PARP-1 (5625), anti-cytochrome c (11940), anti-BAX (2772) anti-p21 Waf1/Cip1 (12D1) and anti-p53 (9282) were purchased from Cell Signaling Technologies. Anti-rabbit or anti-mouse horseradish peroxidase (HRP)-conjugated antibody and anti-β-actin (sc-130656) were purchased from Santa Cruz Biotechnology, Inc.

Anaya-Eugenio G.D., Tan C.Y., Rakotondraibe L.H, & Carcache de Blanco E. (2020). Tumor suppressor p53 independent apoptosis in HT-29 cells by auransterol from Penicillium aurantiacobrunneum. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 127, 110124.

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