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Qiaamp dna stool mini test kit

Manufactured by Qiagen
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Sourced in Germany

The QIAamp® DNA stool mini test kit is a laboratory equipment product that is designed to extract and purify DNA from stool samples. It provides a reliable and efficient method for obtaining high-quality DNA from small amounts of stool material, which can be used in a variety of downstream applications, such as genetic analysis and diagnostics.

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2 protocols using qiaamp dna stool mini test kit

1

Improved Fecal Parasite Recovery

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In order to improve parasite recovery and identification, an aliquot (~1 g) of fresh faecal material was processed using the stool concentration system BioParaprep Mini® (Leti Diagnósticos, Barcelona, Spain) according to the manufacturer’s instructions. Total DNA was extracted from 200 mg of concentrated faecal material using the QIAamp® DNA stool mini test kit (Qiagen, Hilden, Germany). Purified DNA samples (200 μL) were stored at –20°C and shipped to the National Centre for Microbiology, Majadahonda, Spain, for further molecular analysis.
de Lucio A., Amor-Aramendía A., Bailo B., Saugar J.M., Anegagrie M., Arroyo A., López-Quintana B., Zewdie D., Ayehubizu Z., Yizengaw E., Abera B., Yimer M., Mulu W., Hailu T., Herrador Z., Fuentes I, & Carmena D. (2016). Prevalence and Genetic Diversity of Giardia duodenalis and Cryptosporidium spp. among School Children in a Rural Area of the Amhara Region, North-West Ethiopia. PLoS ONE, 11(7), e0159992.
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2

Faecal DNA Extraction Protocol

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Total DNA was extracted from ~200 mg of a new aliquot of faecal material using the QIAamp® DNA stool mini test kit (Qiagen, Hilden, Germany). The extraction was carried out according to the manufacturer’s instructions and the purified DNA (200 μL) was stored at –20°C until further use in downstream molecular analysis.
de Lucio A., Martínez-Ruiz R., Merino F.J., Bailo B., Aguilera M., Fuentes I, & Carmena D. (2015). Molecular Genotyping of Giardia duodenalis Isolates from Symptomatic Individuals Attending Two Major Public Hospitals in Madrid, Spain. PLoS ONE, 10(12), e0143981.
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