Human cytokines were purchased from Peprotech (Rocky Hill, NJ). CD40-his and CD40L were purchased from Cedarlane (Burlington, NC). Isotype and flow cytometry antibodies (CD4-PE, CD8-PE, CD40-APC, CD14-FITC, CD14-PECy7, HLA-DR APC-Cy7 or FITC, CD11c-Alexa Fluor 488, CD86-PE) were obtained from Biolegend or Becton Dickinson (San Jose, CA; CD80-APC, CD83-PE). Rituximab was purchased from InvivoGen (San Diego, CA).
Rituximab
Manufactured by InvivoGen
Rituximab is a chimeric monoclonal antibody that targets the CD20 antigen expressed on the surface of B cells. It is commonly used in various research applications, such as flow cytometry, immunoprecipitation, and immunohistochemistry, to study B cell biology and function.
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Lab products found in correlation
Hla dr apc cy7, by BioLegend (1 mentions)
Cd4 pe, by BioLegend (1 mentions)
Cd8 pe, by BioLegend (1 mentions)
Cd40 apc, by BioLegend (1 mentions)
Cd14 fitc, by BioLegend (1 mentions)
Cd14 pe cy7, by BioLegend (1 mentions)
Fluorescein isothiocyanate (fitc), by BioLegend (1 mentions)
Ofatumumab, by GlaxoSmithKline (1 mentions)
Obinutuzumab, by Roche (1 mentions)
Rituximab, by Roche (1 mentions)
Goat anti mouse igg h l horseradish peroxidase, by Thermo Fisher Scientific (1 mentions)
Ap113p, by Merck Group (1 mentions)
Trastuzumab, by InvivoGen (1 mentions)
4 protocols using rituximab
1
Cytokine and Antibody Profiling Protocol
2
Anti-CD20 Antibody Comparative Study
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The cell lines used in this study were Raji, Jurkat (both human) and the mouse cell line BW5147.
The following anti-CD20 antibodies were used: rituximab and obinutuzumab (Roche), ofatumumab (GSK) and rituximab isotypes (anti-hCD20 isotype collection, InvivoGen). Details of the antibodies used are included in the supplemental methods.
The following anti-CD20 antibodies were used: rituximab and obinutuzumab (Roche), ofatumumab (GSK) and rituximab isotypes (anti-hCD20 isotype collection, InvivoGen). Details of the antibodies used are included in the supplemental methods.
3
Quantifying Serum Levels of Biotherapeutics
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Plasma levels of hG-CSF were analyzed using the hG-CSF Quantikine enzyme-linked immunosorbent assay (ELISA) kit (SCS50; R&D Systems). Serum rituximab levels were analyzed using the rituximab ELISA kit (IG-AB106; Eagle Biosciences). For anti–hG-CSF, 5J8, and anti–IL-5 mAb ELISAs, immunoassay plates were coated with antigen (BioLegend 578606 and Sino Biologicals 11085-V08H and 15673-HNCE, respectively) and blocked with 3% bovine serum albumin. After binding of expressed serum proteins, detection was carried out with enzyme-linked anti-human IgG and 3,3′,5,5′-tetramethylbenzidine substrate (AP113P; Millipore). For the anti-rituximab ELISA, the coat protein was rituximab from InvivoGen, and the detection antibody was goat anti-mouse IgG H+L horseradish peroxidase from Thermo Fisher.
4
Antibody Characterization for Immunotherapy
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Trastuzumab and Rituximab antibodies were purchased from Invivogen: anti-HER2-Tra-hIgG1 (her2tra-mab1), anti-hCD20-hIgG1 (hcd20-mab1), anti-hCD20-hIgG1NQ (hcd20-mab12), anti-hCD20-hIgG2 (hcd20-mab2), anti-hCD20-hIgG3 (hcd20-mab3), anti-hCD20-IgG4 (hcd20-mab4), anti-hCD20-mIgG1 (hcd20-mab9), and anti-CD20-mIgG2a (hcd20-mab10). mIgG2b isotype control clone MPC-11 (559530) was purchased from BD. CR9114, FI6, 2B06, 2G02, 6F12, 8H9, and KB2 were kindly provided by Florian Krammer; see also Table 1 .
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