Cell IL-6 (88-7064, Invitrogen, Carlsbad, CA, USA), IL-8 (EMC104, Neobioscience, Beijing, China) and CXCL1 (EK296/2, MultiSciences Biotech Co., Ltd., Hangzhou, China) levels were measured using a standard quantitative sandwich ELISA kit, according to the manufacturer’s instructions.
Emc104
Manufactured by Neobioscience
Sourced in China
The EMC104 is a laboratory equipment used for the isolation and purification of cellular components such as proteins, nucleic acids, and organelles. It utilizes a combination of centrifugation and chromatographic techniques to separate and concentrate the desired biomolecules from complex biological samples.
Automatically generated - may contain errors
4 protocols using emc104
1
Quantitative Sandwich ELISA of IL-6, IL-8, and CXCL1
2
Quantifying Interleukin-8 in Mouse AT2 Cells
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The concentration of interleukin-8 (IL-8) in the mouse AT2 cell supernatant was measured by ELISA kit (EMC104, NeoBioScience, Shenzhen, China), and the operation process was strictly in accordance with the manufacturing instructions. In general, the antigens were added to the primary antibody and then overlaid with the secondary antibody to form a superimposed pattern. Then after TMB colored, we measured the OD at 450 nm with microplate.
3
EHEC Infection and Host Response
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Fifteen female BALB/c mice (pre-treated with 5 g/L of streptomycin for 3 days) per group were orally gavaged with EHEC WT, ΔataT, or ΔataR [1 × 109 colony-forming units (CFU)]. At the same time, PBS was used as a negative control. Feces from five mice were randomly taken from each group in indicated days, and the bacterial shedding was counted on Sorbitol-MacConkey agar plates until the 23rd day. On the fourth day of infection, blood was collected from five randomly selected mice in each group. We detected mice serum keratinocyte-derived cytokine (KC) concentration using a mouse KC kit (EMC104.96, NeoBioscience, China). We isolated the colon; then fixed, sliced, and stained it; and analyzed pathological lesions.
4
Cimifugin Attenuates LPS-Induced Inflammation
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RAW264.7 cells were divided into a control group (no LPS was added), LPS group (treated with 1 μg/mL LPS for 24 h), high-dose group (1 μg/mL LPS+100 mg/L cimifugin for 24 h), medium-dose group (1 μg/mL LPS+50 mg/L cimifugin for 24 h), and low-dose group (1 μg/mL LPS+25 mg/L cimifugin for 24 h). Cell culture supernatants (100 μL/well) were added into ELISA plates for the determination of the concentrations of IL-6 (EMC004.96; Neobioscience Technology Company, Shenzhen, China), TNF-α (EMC102a.96; Neobioscience Technology Company, Shenzhen, China), IL-1β (EMC001b.96; Neobioscience Technology Company, Shenzhen, China), MIP-2 (EMC122.96; Neobioscience Technology Company, Shenzhen, China), MCP-1 (EMC113.96; Neobioscience Technology Company, Shenzhen, China), and IL-8 (EMC104.96; Neobioscience Technology Company, Shenzhen, China) following the respective manuals. Each sample was tested in triplicate.
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