Anti β catenin antibody sc 7963

NSCLC cells were fixed with 95% ethyl alcohol at room temperature for 30 min and permeabilized with 0.2% Triton X-100 (Beyotime) for 15 min. After blocking with 5% BSA (Solarbio), the cells were incubated with the anti-Setd1A antibody (sc-515,590; Santa Cruz Biotechnology), anti-β-catenin antibody (sc-7963; Santa Cruz Biotechnology) normal rabbit IgG (#2729; Cell Signaling Technology) and normal mouse IgG (A7028; Beyotime) at 4 °C overnight. Next, the cells were incubated with Alexa Fluor 488-labeled Goat Anti-Rabbit IgG (H + L) (A0423; Beyotime) and Cy3-labeled Goat Anti-Mouse IgG (H + L) (A0521; Beyotime) for 1 h at room temperature. After that, the cells were stained with DAPI (C1002; Beyotime) to visualize the nuclei. Finally, the cells were viewed and imaged under a laser scanning confocal microscope (Leica, Bensheim, Germany).