Rabbit peroxidase conjugated secondary antibody

Cultured cells were lysed with lysis buffer. Equal amounts of protein were run on 10% SDS-PAGE and electrotransferred to polyvinylidene fluoride membranes (Millipore). After blocking with 5% milk in TBST, membranes were incubated with primary antibodies overnight. The following antibodies were used: anti-HPVE6 (1:1000, Arigo), anti-HPVE7(1:1000, Bioss), (anti-CXCL10 (1:1,000, Abcam), anti-CXCR3 (1:1,000, Boster), anti-PDL1 (1:2,000, proteintech), anti-STAT1 (1:1,000, proteintech), anti-pSTAT1 (1:1,000, Abcam), anti-JAK1 (1:2,000, proteintech), anti-GAPDH (1:6,000, Yeasen) and anti-tublin (1:3,000, Yeasen). Membranes were then incubated with the rabbit peroxidase-conjugated secondary antibody (1:10,000, Abclonal). The blots were detected by sensitive chemiluminescence liquid analysis (Yeasen) and Biorad software was used to capture the images.

Cultured cells were lysed with lysis buffer. Equal amounts of protein were run on 10 % SDS-PAGE and electrotransferred to polyvinylidene fluoride membranes (Millipore). After blocking with 5 % milk in TBST, membranes were incubated with primary antibodies overnight. The following antibodies were used: anti-ANT3 (1:1000, Proteintech, 14B41-1-AP), anti- FBLN1 (1:1000, Bioss, bs-0809R), E6/E7 (Abcam; ab70, ab30731) and anti-GAPDH (1:6000, Yeasen). Membranes were then incubated with the Rabbit peroxidase-conjugated secondary antibody (1:10,000, Abclonal). The blots were detected by sensitive chemiluminescence liquid (Yeasen), Biorad software was used to captured the images.