Proteins from lysates were prepared and resolved by 12% SDS-PAGE at 100 V, transferred for 1.5 h at 250 mA onto Nitrocellulose membranes, and analysed by immunoblotting as described previously56 (link). The information for primary antibodies is as follows: anti-ferritin (cat# 69090), SdhA (cat# 137040), and SdhB (cat# 178423) from Abcam (Cambridge, MA, USA), anti-Xod (cat# 55156-1-AP), citrate synthase (cat# 16131-1-AP), aconitase 2 (Aco2) (cat# 11134-1-AP), Ndufs1 (cat# 12444-1-AP), Ndufs3 (cat# 15066-1-AP), Uqcrc1 (cat# 21705-1-AP), and Uqcrfs1 (cat# 1843-1-AP) from Proteintech Group Inc. (Chicago, IN, USA), anti-Actin (cat# BM0627) from Boster (Wuhan, China), anti-Tubulin (cat# T0198) from Sigma-Aldrich (St. Louis, MO, USA), anti-TfR1 antibody (cat# 136800) from Zymed (San Francisco, CA, USA), anti-IscU, Fech, Irp1, and Irp2, anti-Fxn (self-made)31 (link), anti-CytC (cat# 1896-1) from Epitmics (Burlingame, CA, USA), anti-Vdac (cat# 4661S) from Cell Signaling Technology Inc (Shanghai, China).
Uqcrfs1
Manufactured by Proteintech
Sourced in United States
UQCRFS1 is an antibody that targets the UQCRFS1 (ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1) protein. UQCRFS1 is a subunit of the mitochondrial respiratory chain complex III, also known as the cytochrome bc1 complex. The UQCRFS1 antibody can be used for the detection and study of this protein in various applications.
Automatically generated - may contain errors
Lab products found in correlation
Ndufs1, by Proteintech (2 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
Anti vdac, by Cell Signaling Technology (1 mentions)
Uqcrc1, by Proteintech (1 mentions)
Anti ferritin, by Abcam (1 mentions)
Anti actin, by Boster Bio (1 mentions)
Anti xod, by Abcam (1 mentions)
Ndufs3, by Proteintech (1 mentions)
Anti tubulin, by Merck Group (1 mentions)
Anti tfr1 antibody, by Zymo Research (1 mentions)
Cleaved caspase 3, by Abcam (1 mentions)
Protease inhibitor cocktail, by Roche (1 mentions)
Anti lc3b, by Merck Group (1 mentions)
Alexa fluor 488 conjugated, by Abcam (1 mentions)
Rapamycin rapa, by Selleck Chemicals (1 mentions)
Parp1, by Proteintech (1 mentions)
Myd88, by Merck Group (1 mentions)
Ndufb8, by Proteintech (1 mentions)
Nao dye, by Merck Group (1 mentions)
Anti sod1, by Proteintech (1 mentions)
3 protocols using uqcrfs1
1
Immunoblotting Analysis of Mitochondrial Proteins
2
Western Blot Analysis of Cellular Proteins
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The harvested cells were lysed in 1% NP-40, 125 mM Tris, pH 6.8, containing a protease inhibitor cocktail (Roche, Basel, Switzerland). For western blotting, 25–50 µg total protein was loaded into 10–12% SDS-PAGE per lane and analyzed by immunoblotting as described previously [46 (link)]. The primary antibodies included: TfR1 antibody (cat#136,800) from Zymed (San Francisco, CA), SDHB (cat#ab178423), cleaved-caspase3 (cat#ab179517) from Abcam (Cambridge, MA), ACO2 (cat#11134-1-AP), NDUFS1 (cat#12444-1-AP), UQCRFS1 (cat#1843-1-AP), NRF2 (cat#16396-1-AP), HO-1 (cat#66743-1-Ig) from Proteintech Group Inc. (Chicago, IN), anti-FTL, FTH, IRP1, and IRP2 (polyclonal, self-made, raised from rabbits) [47 (link)].
3
Oxidative Stress, Autophagy, and Apoptosis
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High‐glucose (25 mM) Dulbecco's modified Eagle's (DMEM‐H), foetal bovine serum (FBS), antibiotics (penicillin and streptomycin), and Lipofectamine2000 were obtained from Thermo Fisher Scientific. Methyl viologen dichloride hydrate (paraquat, PQ), dichloro‐dihydro‐fluorescein diacetate (DCFH‐DA), DAPI, acridine orange (AO), dansylcadaverine (MDC), and NAO dye were purchased from Sigma (MO, USA). Rapamycin (RAPA) and 3‐methyladenine (3‐MA) were purchased from Selleckchem. ST2825 was purchased from MedChemExpress. All molecular biological reagents were obtained from New England Biolabs. Primary antibodies against mTOR, phosphor(P)‐mTOR, P‐p53, Drp1, Tom20, cleaved‐caspase 3, and Bcl‐2 were purchased from Cell Signalling Technology. Anti‐Ki67, phosphorylated histone H2A.X (P‐γH2A.X), CD3, and CD68, and Alexa Fluor® 488‐conjugated or Alexa Fluor® 594‐conjugated second antibody were purchased from Abcam. Anti‐LC3B, SQSTM1/P62, and MyD88 were purchased from Sigma. Anti‐Beclin 1, CDK2, GPX1, 8‐OHdG, OPA1, and Fis1 were purchased from Bioss (Woburn, MA, USA). Anti‐SOD1, p53, PARP1, NDUFB8, and UQCRFS1 were purchased from Proteintech. Secondary antibodies horseradish peroxidase‐conjugated goat anti‐rabbit or mouse IgG was purchased from ZSGB‐Bio.
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