All use of animals conformed to the Association for Research in Vision and Ophthalmology Statement for the Use of Animals in Research and was approved by the Institutional Animal Care and Use Committee and the Institutional Biosafety Committee of the University of California, San Diego, and University of Miami. One- to three-month-old Sprague–Dawley rats, both male and female, were obtained from Harlan Laboratories. GFP mice were bred from the C57BL/6-Tg(CAG-EGFP)1Osb/J strain obtained from Jackson Laboratories. The animals used were randomly selected from groups of similarly aged animals. Because of the novelty of our experiments, no prior estimate of variance was available, so an a priori power analysis was not performed beforehand. However, adequate statistical analysis was performed for each experiment to permit assessment of statistical significance.
C57bl 6 tg cag egfp 1osb j strain
Manufactured by Jackson ImmunoResearch
Sourced in United States
The C57BL/6-Tg(CAG-EGFP)1Osb/J strain is a transgenic mouse model that expresses enhanced green fluorescent protein (EGFP) under the control of the chicken beta-actin promoter and CMV immediate-early enhancer. This strain is commonly used for various research applications where visualization of cells and tissues expressing EGFP is required.
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Lab products found in correlation
2 protocols using c57bl 6 tg cag egfp 1osb j strain
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Animal Handling and Experimental Protocols
2
Transgenic Mouse and Rat Protocols
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All use of animals conformed to the ARVO Statement for the Use of Animals in Research and was approved by the Institutional Animal Care and Use Committee (IACUC) and the Institutional Biosafety Committee of Stanford University. GFP+ mice, both male and female, were bred from the C57BL/6-Tg(CAG-EGFP)1Osb/J strain obtained from Jackson Laboratories (Bar Harbor, ME, USA). Ten- to 12-week-old Sprague-Dawley (SD) rats, both male and female, were obtained from Charles River (Portage, MI, USA). Human iPSC line 297 (iPSC/297), derived from blood cells with Sendai virus transfection (Cytotune 2.0 kit; Life Technologies Inc., Gaithersburg, MD, USA) were kindly provided by the Stem Cell Core, Stanford University Department of Genetics (Stanford, CA, USA).
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