HFF were washed with PBS and fixed in 3% formaldehyde at room temperature for 15 min. After washing with PBS twice, HFF were incubated overnight at 37 °C with β-galactosidase staining reagents (1 mg/mL X-gal, 2 mM MgCl2, 150 mM NaCl, pH 6.0, 5 mM potassium ferrocyanide, 5 mM potassium ferricyanide and 40 nM citric acid/sodium phosphate: Beyotime, Shanghai, China). Eventually, stained HFF were imaged with a light microscope (Nikon, Tokyo, Japan) at 50× magnification, and the percentage of positive cells were analyzed with Image-Pro Plus 6.0 (Media Cybernetics, Silver Spring, MD, USA). All experiments were performed three times.
Potassium ferrocyanide
Manufactured by Beyotime
Sourced in China
Potassium ferrocyanide is a chemical compound with the formula K4[Fe(CN)6]. It is a yellow, crystalline solid that is soluble in water. Potassium ferrocyanide is commonly used as a reagent in analytical chemistry and as a component in various laboratory applications.
Automatically generated - may contain errors
2 protocols using potassium ferrocyanide
1
Senescence-Associated β-Galactosidase Staining
2
Enhanced Perls Staining of Brain Sections
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We prepared paraffin coronal brain sections (5 µm thick) for enhanced Perls staining. Brain sections with hematoma were incubated in Perls solution (10% potassium ferrocyanide and 10% HCl in a 7:3 ratio with 0.1% Triton X-100; Beyotime Biotechnology) for 30 minutes at room temperature (Yang et al., 2020). The sections were washed in distilled water and incubated in 0.5% diamine benzidine tetrahydrochloride with nickel (Beyotime Biotechnology) for 60 minutes. Images were captured using a confocal microscope (LSM 880, Carl Zeiss) in a blinded manner.
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