Tissue homogenization and sonication were carried out by means of a Wheaton® 903475 Overhead Stirrer apparatus (Wheaton, Millville, NJ, USA) and a Branson Sonifier 450 (Branson Ultrasonics, Danbury, CT, USA), respectively. Total protein concentration was determined in duplicate by Bradford assay (Bio-Rad Laboratories, Hercules, CA, USA) and UV–Vis spectrophotometer (8453 UV–Vis Supplies, Agilent Technologies, Waldbronn, Germany) detector using BSA as the protein of reference. For sample centrifugation, a thermostated centrifuge SL16 R (Thermo Fisher Scientific, Langenselbold, Germany) or Mini Spin (Eppendorf AG, Hamburg, Germany) were used as specified for sample treatment. HPLC–ESI–MS/MS analyses were performed on an UltiMate 3000 RSLCnano System (Dionex, Sunnyvale, CA, USA) coupled with an Orbitrap Elite MS detector with ESI or EASY-Spray nanoESI sources (Thermo Fisher Scientific), as specified elsewhere.
Easy spray nanoesi
Manufactured by Thermo Fisher Scientific
Sourced in United States
The EASY-Spray nanoESI is a laboratory instrument designed for nanospray electrospray ionization (nanoESI) applications. It provides a reliable and consistent method for sample introduction into mass spectrometers. The core function of the EASY-Spray nanoESI is to generate a stable and reproducible electrospray of sample solutions for mass spectrometry analysis.
Automatically generated - may contain errors
Lab products found in correlation
Ultimate 3000 rslcnano system, by Thermo Fisher Scientific (2 mentions)
Sl16r, by Thermo Fisher Scientific (2 mentions)
Minispin, by Eppendorf (2 mentions)
Orbitrap elite ms detector, by Thermo Fisher Scientific (2 mentions)
Bradford assay, by Bio-Rad (2 mentions)
Branson sonifier 450, by Emerson (1 mentions)
Uv vis spectrophotometer 8453 uv vis supplies, by Agilent Technologies (1 mentions)
2 protocols using easy spray nanoesi
1
Protein Extraction and Quantification
2
Proteomic Analysis of Tissue Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Tissue homogenization and sonication were carried out by means of Wheaton® 903475 Overhead Stirrer apparatus (Wheaton, Millville, NJ, USA) and Branson sonifer 450 (Branson Ultrasonics, Danbury, CT, USA), respectively. Total protein concentration was determined in duplicate by Bradford assay (Bio-Rad Laboratories, Hercules, CA, USA) and UV-Vis spectrophotometer (8453 UV-Vis Supplies, Agilent Technologies, Waldbronn, Germany) detector using BSA as the protein of reference. For sample centrifugation, thermostated centrifuge SL16 R (Thermo Fisher Scientific, Langenselbold, Germany) and Mini Spin (Eppendorf AG, Germany) were used as specified for sample treatment. HPLC-ESI-MS/MS analyses were performed on UltiMate 3000 RSLCnano System (Dionex, Sunnyvale, CA, USA) coupled to Orbitrap Elite MS detector with ESI or EASY-Spray nanoESI sources (Thermo Fisher Scientific), as specified elsewhere.
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