Dna loading buffer

HPLC-purified oligonucleotides used in this research (as listed in Table S1) were supplied by Sangon Biotech Inc. (Shanghai, China) and fully dissolved in an ice box with 1 × TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0) at a storage concentration of 10 μM.
The 1 × TE buffer, 10 mM deoxynucleotide triphosphates (dNTPs), 6 × DNA loading buffer, 30% acrylamide/bis solution, and ammonium persulfate (APS) were obtained from Sangon Biotechnology Co. Ltd (Shanghai, China).
N,N,N′,N′-Tetramethylethylenediamine (TEMED) and 20 bp DNA Ladder (Dye Plus) used for polyacrylamide gel electrophoresis was acquired from TaKaRa Biotech (Dalian, China). GoldView was acquired from Solarbio LIFE SCIENCES (Beijing, China). ThT was purchased from BBI LIFE SCIENCES CORPORATION (Shanghai, China) and dissolved in ultrapure water to 100 μM for fluorescent measurements. Nb.BbvCI endonuclease and Klenow fragment (3′-5′ exo-) polymerase (KF polymerase) used in this study were provided by New England Biolabs (Beijing, China). Ultrapure water used for solution preparation was obtained from a Millipore water purification system with a resistivity of 18.2 MΩ cm. Human serum samples for the recovery experiment were obtained from the First Affiliated Hospital of Chongqing Medical University.

The primers (Table 1) used in this study were synthesized and purified by Sangon Biotech (Shanghai, China). The colorimetric and fluorescent detection kit were provided by Qingdao Navid Biotechnology Co., Ltd. (Qingdao, China). 6× DNA loading buffer, 20 bp DNA marker, ethidium bromide were purchased from Sangon Biotech (Shanghai, China). The raw meat used in this study was purchased from local supermarket.