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Si mate

Manufactured by GenePharma
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Sourced in China

Si-MateTM is a laboratory equipment product designed for sample preparation. It serves as a versatile tool for mixing and homogenizing various types of samples, ensuring consistent and efficient sample handling during analytical processes.

Automatically generated - may contain errors

Lab products found in correlation

Fugene 6, by Promega (2 mentions) Dual luciferase assay system, by Promega (1 mentions) Mir 328 3p mimic, by GenePharma (1 mentions) Mirna nc, by GenePharma (1 mentions)

3 protocols using si mate

1

Dual-Luciferase Assay for miRNA-H2AFX Interaction

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Cells were seeded into 24-well plate, and co-transfected with 50 nM of either miR-328-3p mimics or NC oligos in combination with 150 ng of wild-type or mutated 3'-UTR H2AFX plasmids (GeneCopoeia Inc., MD, USA) via si-MateTM (Genepharma) and the FuGENE® 6 (Promega, Madison, WI, USA), respectively. Then, the luciferase activity was appraised through utilizing the dual-luciferase assay system (Promega Corporation, WI) on the basis of the manufacturer's information.
Ma H., Liu C., Zhang S., Yuan W., Hu J., Huang D., Zhang X., Liu Y, & Qiu Y. (2021). miR-328-3p promotes migration and invasion by targeting H2AFX in head and neck squamous cell carcinoma. Journal of Cancer, 12(21), 6519-6530.
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2

Modulating miR-328-3p and H2AFX in HNSCC

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Tca8113 and FaDu cells were transfected with miR-328-3p mimics (Genepharma, Suzhou, China) or negative control (NC), while JHU011 and Tu686 cells were transfected with miR-328-3p anti-sense molecules (also called inhibitor) or NC, using the si-MateTM (Genepharma) according to the manufacturer's protocol. Tca8113 and FaDu cells were transfected with H2AFX cDNA plasmid (EX-B0074-M98, GeneCopoeia Inc., MD, USA) using the FuGENE® 6 (Promega, Madison, WI, USA).
Ma H., Liu C., Zhang S., Yuan W., Hu J., Huang D., Zhang X., Liu Y, & Qiu Y. (2021). miR-328-3p promotes migration and invasion by targeting H2AFX in head and neck squamous cell carcinoma. Journal of Cancer, 12(21), 6519-6530.
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3

Modulating miR-93-5p Expression in SCCHN

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SCCHN cells were transfected with the miR-93-5p mimics, the miR-93-5p inhibitors, or with negative control (NC) miRNA (Genepharma, Suzhou, China) using si-MateTM (Genepharma) according to the manufacturer's protocol. Additionally, to establish a cell line that stably expressed miR-93-5p, Tu686 cells were infected with lentivirus expressing miR-93-5p (GeneCopoeia Inc., MD, USA), and the infected cells were selected in the medium containing 3 μg/mL puromycin.
Zhang S., He Y., Liu C., Li G., Lu S., Jing Q., Chen X., Ma H., Zhang D., Wang Y., Huang D., Tan P., Chen J., Zhang X., Liu Y, & Qiu Y. (2020). miR-93-5p enhances migration and invasion by targeting RGMB in squamous cell carcinoma of the head and neck. Journal of Cancer, 11(13), 3871-3881.
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