The tumor and paired normal samples were collected from three lung adenocarcinoma patients with written informed consent. Total RNA was prepared by the PureLink Total RNA purification kit (Invitrogen, USA). The quality and integrity of RNA were accessed by the Qubit3.0 Fluorometer (Life Technologies, USA) and Agilent 2100 RNA Nano 6000 Assay Kit (Agilent Technologies, USA). Three micrograms of RNAs per sample were used for the following steps. The rRNA in the library were removed by Ribo-Zero GoldKits (Illumina, USA). Twelve libraries for six samples with 2 replicates each were constructed using NEBNext Ultra DirectionalRNA Library Prep Kit for Illumina (NEB, USA) according to the product instruction. They were finally sequenced by Illumina HiSeq2500 for 150 bp pair-end sequencing.
Purelink total rna purification kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The PureLink Total RNA Purification Kit is a laboratory product designed to efficiently isolate total RNA from a variety of sample types, including cells, tissues, and bacteria. The kit utilizes a silica-based membrane technology to capture and purify the RNA, allowing for high-quality RNA extraction suitable for downstream applications such as RT-PCR, Northern blotting, and microarray analysis.
Automatically generated - may contain errors
Lab products found in correlation
Ribo zero gold kit, by Illumina (1 mentions)
Agilent 2100 rna nano 6000 assay kit, by Agilent Technologies (1 mentions)
Qubit 3.0 fluorometer, by Thermo Fisher Scientific (1 mentions)
Hiseq 2500, by Illumina (1 mentions)
Agpath id one step rt pcr reagent, by Thermo Fisher Scientific (1 mentions)
Quant it ribogreen rna reagent, by Thermo Fisher Scientific (1 mentions)
Quantstudio 6 and 7 flex real time pcr system, by Thermo Fisher Scientific (1 mentions)
2 protocols using purelink total rna purification kit
1
Profiling Lung Adenocarcinoma Transcriptomes
2
RNA Extraction and RT-qPCR Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cultured CFF-16HBEge cells and hBE cells were lysed following ASO treatments and total RNA was prepared using the PureLink Total RNA Purification Kit as per the manufacturer’s instructions (Invitrogen, Carlsbad, CA). The RT-qPCR analysis was performed with QuantStudio 6 and 7 Flex real-time PCR systems (Applied Biosystems, Foster City, CA) using AgPath-ID One-Step RT-PCR reagents (Applied Biosystems, Foster City, CA). RT-qPCR results were normalized to quantified total RNA using the Quant-iT Ribogreen RNA reagent (Molecular Probes, Eugene, OR) and to housekeeping gene β-actin. TaqMan primer-probe set sequences are provided (Supplementary Table 1 ).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!