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Her3 erbb3 xp

Manufactured by Cell Signaling Technology
Sourced in United States

Her3/ERBB3 XP is a high-quality recombinant protein that can be used for research purposes. It is a member of the epidermal growth factor receptor (EGFR) family and plays a role in cell signaling pathways.

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2 protocols using her3 erbb3 xp

1

Investigating ERBB3 Signaling Pathway

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The following antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA); MITF (1:1000; #12590), Her3/ERBB3 XP (1:1000; #12708), Phospho-Her3/ERBB3-Tyrosine-1289 (1:1000; #4791), FOXD3 (1:1000; #2019), SOX10 (1:1000; #14374), Akt (1:2000; #9272), Phospho-Akt-Serine-473 XP (1:2000; #4060), and Histone H3 (1:3000; #4499) was used as loading control. Secondary antibody against rabbit (1:5000; P0448) was purchased from Dako (Agilent Technologies, Glostrup, Denmark). The small molecular inhibitor vemurafenib (Plexxikon 4032) was obtained from Selleck Chemicals (Houston, TX, USA). The NRG1-beta ligand was purchased from ImmunoTools GmbH (Friesoythe, Germany). The NRG1-beta ligand was used to enhance the pERBB3 (T1289) signal, as our Phospho-Her3/ERBB3-Tyrosine-1289 antibody did not detect pERBB3 (T1289) levels without NRG1-beta addition (see also Supplementary Figure S3A-S3B). The reason for choosing 15min of incubation time and a NRG1-beta ligand concentration of 10ng/ml was due to a previous study where they showed efficient activation of the ERBB3 receptor in the WM115 cell line under these conditions [18 (link)].
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2

Antibody-Based Protein Quantification

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Antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA); MITF (1:1000; #12590), Her3/ERBB3 XP (1:1000; #12708), AXL (1:1000 #8661), SOX10 (1:1000; #14374), Phospho‐Akt‐Serine‐473 XP® (1:2000; #4060), Phospho‐p44/42 (ERK1/2) XP® (1:2000; #4370S) and Histone H3 (1:3000; #4499) was used as a loading control. Secondary antibody against rabbit (1:5000; P0448) was purchased from Dako (Agilent Technologies, Glostrup, Denmark). The small molecular inhibitors vemurafenib (Plexxikon 4032), AZD8931 (Sapatinib) and R428 (BGB324/bemcentinib) were obtained from Selleck Chemicals (Houston, TX, USA). Recombinant human NRG1‐beta protein‐ligand was purchased from ImmunoTools GmbH (Friesoythe, Germany). Recombinant human GAS6 protein‐ligand was purchased from R&D Systems (Minneapolis, USA).
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