Protease inhibitor cocktail set 1

Manufactured by BestBio

Protease inhibitor cocktail Set I is a premixed solution designed to inhibit a broad spectrum of serine, cysteine, and metalloproteases. It is suitable for use in cell and tissue extracts, as well as other biological samples, to prevent degradation of target proteins during sample preparation and analysis.

Automatically generated - may contain errors

Lab products found in correlation

Odyssey detection system, by LI COR (2 mentions) Ab170904, by Abcam (1 mentions) β actin mab, by Santa Cruz Biotechnology (1 mentions) Ripa lysis buffer, by BestBio (1 mentions) Ab6992, by Abcam (1 mentions) Ab16663, by Abcam (1 mentions) Mouse anti gapdh antibody, by Santa Cruz Biotechnology (1 mentions)

2 protocols using protease inhibitor cocktail set 1

Western Blot Analysis of Protein Expression

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We extracted total protein from treated cells or tissues using RIPA lysis buffer (BestBio) containing protease inhibitor cocktail Set I (BestBio). We separated proteins in 10% precast SDS-PAGE gels and transferred them onto a nitrocellulose membrane. The membrane was further incubated with primary P-glycoprotein mAb (1:100 dilution; ab170904, Abcam), ZEB1 mAb (1:100 dilution; 21544–1-AP, ProteinTech) or β-actin mAb (1:1000 dilution; Santa Cruz, CA, USA). We visualized the blots using an odyssey detection system (LI-COR Biosciences, Lincoln, NE, USA) and quantified protein abundance by ImageJ software.

Huang C., Huang S., Li H., Li X., Li B., Zhong L., Wang J., Zou M., He X., Zheng H., Si X., Liao W., Liao Y., Yang L, & Bin J. (2018). The effects of ultrasound exposure on P-glycoprotein-mediated multidrug resistance in vitro and in vivo. Journal of Experimental & Clinical Cancer Research : CR, 37, 232.

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Western Blot Analysis of Cell Proteins

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Total protein from cells or tissue lysates was prepared using radioimmunoprecipitation assay (RIPA) lysis buffer (boster biological technology [BestBio]) containing Protease Inhibitor Cocktail Set I (BestBio). Proteins were separated in 10% SDS-PAGE gels and transferred to nitrocellulose membranes. The following primary antibodies were used: anti-Ccnd1 (ab16663; Abcam), anti-N1ICD (#4147; Cell Signaling Technology [CST]), and anti-CTGF (ab6992; Abcam). As a control, we used the mouse anti-Gapdh antibody (1:1,000 dilution; Santa Cruz Biotechnology, Santa Cruz, CA, USA). Alexa Fluor 680-conjugated anti-rabbit immunoglobulin G (IgG; 1:10,000 dilution; Abcam, USA) was used as the secondary antibody. Signals were captured by an Odyssey detection system (LI-COR Biosciences, Lincoln, NE, USA).

Si X., Zheng H., Wei G., Li M., Li W., Wang H., Guo H., Sun J., Li C., Zhong S., Liao W., Liao Y., Huang S, & Bin J. (2020). circRNA Hipk3 Induces Cardiac Regeneration after Myocardial Infarction in Mice by Binding to Notch1 and miR-133a. Molecular Therapy. Nucleic Acids, 21, 636-655.

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