Tumor cells were inoculated into 6-well plates and treated with drugs for 2 hours according to the experimental groups. The BODIPY 581/591-C11 lipid peroxidation fluorescent probe (10 µM; Invitrogen, USA) was added. After the cells were incubated at 37°C for 30 min, flow cytometry analysis was performed. For BODIPY 581/591 C11 staining, the signals from both non-oxidized C11 (the phycoerythrin (PE) channel) and oxidized C11 (the fluorescein isothiocyanate (FITC) channel) were monitored. The ratio of the mean fluorescence intensity (MFI) of FITC to the MFI of PE was calculated for each sample. The data were normalized to control samples.
Bodipy 581 591 c11 lipid peroxidation fluorescent probe
Manufactured by Thermo Fisher Scientific
Sourced in United States
BODIPY 581/591-C11 is a fluorescent probe that can be used to detect lipid peroxidation. It is a membrane-permeable compound that incorporates into lipids and undergoes a shift in its fluorescence emission spectrum upon oxidation.
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Lab products found in correlation
2 protocols using bodipy 581 591 c11 lipid peroxidation fluorescent probe
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Lipid Peroxidation Assay in Tumor Cells
2
Lipid Peroxidation Assay in Tumor Cells
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Tumor cells were inoculated into 6-well plates and treated with drugs for 2 hours according to the experimental groups. The BODIPY 581/591-C11 lipid peroxidation fluorescent probe (10 µM; Invitrogen, USA) was added. After the cells were incubated at 37°C for 30 min, flow cytometry analysis was performed. For BODIPY 581/591 C11 staining, the signals from both non-oxidized C11 (the phycoerythrin (PE) channel) and oxidized C11 (the fluorescein isothiocyanate (FITC) channel) were monitored. The ratio of the mean fluorescence intensity (MFI) of FITC to the MFI of PE was calculated for each sample. The data were normalized to control samples.
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