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Alexa fluor labeled secondary goat anti rabbit antibody

Manufactured by Thermo Fisher Scientific

Alexa Fluor-labeled secondary goat anti-rabbit antibody is a fluorescently-conjugated antibody that binds to rabbit primary antibodies. It is designed for use in immunoassays, immunocytochemistry, and other applications requiring detection of rabbit-specific targets.

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2 protocols using alexa fluor labeled secondary goat anti rabbit antibody

1

Quantification of Placental Monoamine Oxidase

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Monoamine oxidase protein expression was quantified in frozen sections of human placenta using the MAO-A (Abcam, ab126751), MAO-B (Abcam, ab175136) primary antibody, and Alexa Fluor-labeled secondary goat anti-rabbit antibody (Invitrogen, A32731), respectively, as previously described [17 (link)]. Nuclear staining was obtained with DAPI (Santa Cruz, SC3598). The slides were examined using the Olympus Fluoview FV1000 confocal microscope. Quantification of MAO was performed by measurement of fluorescence intensity (expressed as arbitrary units) using ImageJ software.
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2

Quantification of Atrial MAO Isoforms

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Tissue expression of MAOs isoforms was quantified in frozen atrial sections using the MAO-A (Abcam, ab126751) and MAO-B (Abcam, ab175136) primary antibodies and Alexa Fluor-labeled secondary goat anti-rabbit antibody (Invitrogen, A32731), while DAPI (Santa Cruz, SC3598) was used for the nuclear staining. The slides were examined in confocal microscopy (Olympus Fluoview FV1000) and image analysis was performed using the Image J software.
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