The SARS-CoV-2 Spike S1-RBD IgG and IgM ELISA Detection kit was used to determine levels of IgG and IgM antibodies in the plasma of COVID-19 and control subjects according to the manufacturer’s instructions (GenScript, Piscataway, NJ). Monoclonal IgG and IgM antibodies specific to SARS-CoV-2 were used as positive controls. The concentration of S1 IgG in the COVID-19 convalescent plasma was quantitated using a high-sensitive SARS-CoV-2 S1 IgG ELISA kit (BioVendor, Asheville, NC).
Sars cov 2 spike s1 rbd igg and igm elisa detection kit
Manufactured by GenScript
The SARS-CoV-2 Spike S1-RBD IgG and IgM ELISA Detection kit is a laboratory equipment product designed to detect IgG and IgM antibodies against the SARS-CoV-2 Spike S1-RBD protein. The kit utilizes the Enzyme-Linked Immunosorbent Assay (ELISA) technique to measure the levels of these specific antibodies in human serum or plasma samples.
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4 protocols using sars cov 2 spike s1 rbd igg and igm elisa detection kit
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SARS-CoV-2 Spike S1-RBD Antibody ELISA
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SARS-CoV-2 S-RBD Antibody ELISA
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To evaluate antibodies binding to CoV-2 S-RBD protein, SARS-CoV-2 Spike S1-RBD IgG and IgM ELISA Detection Kit from GenScript was used according to the manufacturer’s instructions. Absorbance was measured at 450 nm using an Epoch 2 microplate spectrophotometer (BioTek).
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SARS-CoV-2 Antibody and Neutralization Assay
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For the measurement of anti-SARS-CoV-2 RBD IgG levels in plasma samples, SARS-CoV-2-Spike S1-RBD IgG and IgM ELISA Detection Kit (GenScript) was used according to the manufacturer’s instruction. For the measurement of SARS-CoV-2 neutralizing activities of plasma samples, SARS-CoV-2 Surrogate Virus Neutralization Detection Kit (GenScript) was used according to the manufacturer’s instruction. The percent of the neutralizing activity was calculated with the following equation.
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SARS-CoV-2 Antibody Titer Determination
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Anti‐RBD IgG and IgM antibody titers were determined using a SARS‐CoV‐2 Spike S1‐RBD IgG and IgM ELISA detection kit (Genscript). Anti‐N IgG and IgM antibody titers were determined using a SARS‐CoV‐2 Nucleocapsid Protein IgG ELISA Kit (Lifeome). Both kits were optimized by replacing the HRP‐conjugated IgG or IgM anti‐human antibody with an HRP‐conjugated IgG (H + L) goat anti‐mouse antibody (Thermo Fisher Scientific) or an HRP‐conjugated IgM (heavy chain) goat anti‐mouse antibody (Thermo Fisher Scientific), respectively. Immunoglobulin isotyping was evaluated using Ig Isotyping Mouse Uncoated ELISA Kit (Thermo Fisher Scientific) and IgE Mouse ELISA kit (Thermo Fisher Scientific) following the manufacturer's recommendation by measuring absorbance at 450 nm on a plate reader (Synergy HT). All ELISAs were performed on mouse sera that were heat‐inactivated for 30 min at 56 °C. Endpoint titers were determined as the maximum dilution that emitted an optical density exceeding 4 times the background (i.e., sera from mice vaccinated with Sham vaccine).
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