Fetal membrane tissues (E13, E15, E17, E18, E19) collected from mice were assayed for IL-6, IL-8, IL-10 and IL-1β using MILLIPLEX Mouse High Sensitivity T Cell Magnetic Bead Panel (Millipore) following the manufacturer’s protocol. Equal amounts of protein (30 μg) were loaded into each well. Standard curves were developed using duplicate samples of known-quantity recombinant proteins that were provided by the manufacturer. Sample concentrations were determined by relating the absorbance of the samples to the standard curve using linear regression analysis.
Milliplex mouse high sensitivity t cell magnetic bead panel
Manufactured by Merck Group
Sourced in United States
The MILLIPLEX Mouse High Sensitivity T Cell Magnetic Bead Panel is a laboratory equipment product that enables the simultaneous detection and quantification of multiple T cell-related analytes in mouse samples. It utilizes magnetic beads coated with specific antibodies to capture and measure the levels of various T cell markers.
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Lab products found in correlation
2 protocols using milliplex mouse high sensitivity t cell magnetic bead panel
1
Cytokine Profiling of Fetal Membranes
2
Blood Collection and Cytokine Analysis
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Immediately following blood collection at euthanasia, blood was kept at room temperature for 30 min to allow clotting, then put on ice before centrifugation. The blood was centrifuged for 10 min at 10,000× g at 4 °C, after which the serum was collected and separated into 50 μL aliquots and stored at −80 °C until use. Additionally, the spleen was excised and weighed to determine the relative spleen weight, which may be used as a marker of an increased immune response [49 (link)].
The serum concentrations of IL-1β, TNF-α, and IL-10 were measured by a custom Milliplex Mouse High Sensitivity T Cell Magnetic Bead Panel (Millipore, Burlington, MA, USA; MHSTCMAG-70K-03), as per the manufacturer’s instructions. Of note, the concentration of IL-1β in most samples were below detectable limit, and, thus, were excluded from this study. The resultant plate was then read by the Magpix Multiplex Reader (Luminex, Austin, TX, USA), and data were acquired using Luminex xPonent for Magpix (v4.2), then analyzed by Milliplex analyst (v5.1.0.0) (VigeneTech Inc., Carlisle, MA, USA) and presented as concentration (pg/mL).
The serum concentrations of IL-1β, TNF-α, and IL-10 were measured by a custom Milliplex Mouse High Sensitivity T Cell Magnetic Bead Panel (Millipore, Burlington, MA, USA; MHSTCMAG-70K-03), as per the manufacturer’s instructions. Of note, the concentration of IL-1β in most samples were below detectable limit, and, thus, were excluded from this study. The resultant plate was then read by the Magpix Multiplex Reader (Luminex, Austin, TX, USA), and data were acquired using Luminex xPonent for Magpix (v4.2), then analyzed by Milliplex analyst (v5.1.0.0) (VigeneTech Inc., Carlisle, MA, USA) and presented as concentration (pg/mL).
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